[Objective]To study the effects of homologous chromosomes 7 (7A,7B and 7D) on wheat photosynthesis and provide theoretical basis for breeding high photosynthetic efficiency wheat by genetic,physiological and biochem...[Objective]To study the effects of homologous chromosomes 7 (7A,7B and 7D) on wheat photosynthesis and provide theoretical basis for breeding high photosynthetic efficiency wheat by genetic,physiological and biochemical means. [Method]The Triticum asetivum cultivar Chinese Spring wheat and nullisomic wheat (N7A,N7B and N7D) were planted in greenhouse. The photosynthetic indexes were determined at early filling stage. [Result]The photosynthetic rate (Pn),stomatal conductance (Gs),primary photochemical efficiency (Fv/Fm),actual chemical efficiency of photosystem II (ФPS II) and apparent electron transfer rate of photosystem II (ETR) were significantly lower in the N7A and N7B than in the Chinese Spring (P0.05). The photosynthetic rate and stomatal conductance was significantly lower in the N7D than in the Chinese Spring (P0.05). The Fv/Fm,ФPS II,ETR of N7D were higher than that of the Chinese Spring without significant difference. [Conclusion]The homologous chromosomes 7A and 7B have positive effects on photosynthetic rate and have relationship with stomatal conductance and photoreaction (chlorophyll fluorescence parameters). The homologous chromosome 7D has negative effects on photosynthetic rate,which is mainly related to stomatal conductance rather than photoreaction.展开更多
AIM: To evaluate the feasibility and utility of confocal laser endomicroscopy (CLE) in the description of normal gastrointestinal (GI) mucosa and in the diagnosis of GI disorders in children, in comparison to his...AIM: To evaluate the feasibility and utility of confocal laser endomicroscopy (CLE) in the description of normal gastrointestinal (GI) mucosa and in the diagnosis of GI disorders in children, in comparison to histology.METHODS: Forty-four patients (19 female) median age 10.9 years (range 0.7-16.6 years) with suspected or known GI pathology underwent esophago-gastro- duodenoscopy (OGD) (n = 36) and/or ileocolonoscopy (IC) (n = 31) with CLE using sodium fluorescein and acriflavine as contrast agents. Histological sections were compared with same site confocal images by two experienced pediatric and GI histopathologists and endoscopists, respectively.RESULTS: Duodenum and ileum were intubated in all but one patient undergoing OGD and IC. The median procedure time was 16.4 min (range 7-25 rain) for OGD and 27.9 min (range 15-45 min) for IC. A total of 4798 confocal images were compared with 153 biopsies from the upper GI tract from 36 procedures, and 4661 confocal images were compared with 188 biopsies from the ileocolon from 31 procedures.Confocal images were comparable to conventional histology both in normal and in pathological conditions such as esophagitis, Helicobacter pylori gastritis, celiac disease, inflammatory bowel disease, colonic heterotopia, and graft versus host disease.CONCLUSION: CLE offers the prospect of targeting biopsies to abnormal mucosa, thereby increasing diagnostic yield, reducing the number of biopsies, decreasing the burden on the histopathological services, and reducing costs.展开更多
基金Supported by Excellent Young Academic Leaders Project of Shanxi Province~~
文摘[Objective]To study the effects of homologous chromosomes 7 (7A,7B and 7D) on wheat photosynthesis and provide theoretical basis for breeding high photosynthetic efficiency wheat by genetic,physiological and biochemical means. [Method]The Triticum asetivum cultivar Chinese Spring wheat and nullisomic wheat (N7A,N7B and N7D) were planted in greenhouse. The photosynthetic indexes were determined at early filling stage. [Result]The photosynthetic rate (Pn),stomatal conductance (Gs),primary photochemical efficiency (Fv/Fm),actual chemical efficiency of photosystem II (ФPS II) and apparent electron transfer rate of photosystem II (ETR) were significantly lower in the N7A and N7B than in the Chinese Spring (P0.05). The photosynthetic rate and stomatal conductance was significantly lower in the N7D than in the Chinese Spring (P0.05). The Fv/Fm,ФPS II,ETR of N7D were higher than that of the Chinese Spring without significant difference. [Conclusion]The homologous chromosomes 7A and 7B have positive effects on photosynthetic rate and have relationship with stomatal conductance and photoreaction (chlorophyll fluorescence parameters). The homologous chromosome 7D has negative effects on photosynthetic rate,which is mainly related to stomatal conductance rather than photoreaction.
基金Supported by Peel Research Foundation and Yorkshire Cancer ResearchThe Egyptian Cultural Bureau
文摘AIM: To evaluate the feasibility and utility of confocal laser endomicroscopy (CLE) in the description of normal gastrointestinal (GI) mucosa and in the diagnosis of GI disorders in children, in comparison to histology.METHODS: Forty-four patients (19 female) median age 10.9 years (range 0.7-16.6 years) with suspected or known GI pathology underwent esophago-gastro- duodenoscopy (OGD) (n = 36) and/or ileocolonoscopy (IC) (n = 31) with CLE using sodium fluorescein and acriflavine as contrast agents. Histological sections were compared with same site confocal images by two experienced pediatric and GI histopathologists and endoscopists, respectively.RESULTS: Duodenum and ileum were intubated in all but one patient undergoing OGD and IC. The median procedure time was 16.4 min (range 7-25 rain) for OGD and 27.9 min (range 15-45 min) for IC. A total of 4798 confocal images were compared with 153 biopsies from the upper GI tract from 36 procedures, and 4661 confocal images were compared with 188 biopsies from the ileocolon from 31 procedures.Confocal images were comparable to conventional histology both in normal and in pathological conditions such as esophagitis, Helicobacter pylori gastritis, celiac disease, inflammatory bowel disease, colonic heterotopia, and graft versus host disease.CONCLUSION: CLE offers the prospect of targeting biopsies to abnormal mucosa, thereby increasing diagnostic yield, reducing the number of biopsies, decreasing the burden on the histopathological services, and reducing costs.