A full_length cDNA has been cloned encoding nicotinamide adenine dinucleotide phosphate_specific glutamate dehydrogenase (NADP_GDH) from Chlorella sorokiniana with the RT_PCR method. The complete nucleotide sequence o...A full_length cDNA has been cloned encoding nicotinamide adenine dinucleotide phosphate_specific glutamate dehydrogenase (NADP_GDH) from Chlorella sorokiniana with the RT_PCR method. The complete nucleotide sequence of NADP_GDH gene had 94% homology to the previously reported one . The NADP_GDH gene was constructed into a vector highly expressed in plants. The specific activity of NADP_GDH in transformants was detected, but not in the control plants. All transformed shoots on MS medium containing lower concentration of nitrogen and the transformed seedlings grown in lower concentration of nitrogen vermiculite had higher growth rate and more leaves than the control plants. Transformed leaf discs cultured on MS medium containing different nitrogen concentrations had more chlorophyll contents compared to the controls. These results suggested that exogenous NADP_GDH may enhance the absorption and utilization to ammonium in plants. The increased weight of transformed leaf discs cultured on medium supplemented with different concentrations of phosphinothricin (PPT) was more than that of control discs. 0.5 μg/mL PPT could be used as a selecting drug instead of kanamycin to develop the transformants. These results suggested that the NADP_GDH gene might be used as a new selecting gene in the future research of plant gene engineering.展开更多
130 wheat varieties are selected as materials to test the LOX activity, whiteness, yellowness, carotenoids content and RVA parameters, which are to be analyzed by variance analysis, correlation analysis, cluster analy...130 wheat varieties are selected as materials to test the LOX activity, whiteness, yellowness, carotenoids content and RVA parameters, which are to be analyzed by variance analysis, correlation analysis, cluster analysis and classified according to the origins. The result shows there are highly significant variations in LOX activity from varieties; LOX activity is positively correlated with whiteness, and significantly and negatively correlated with yellowness. Cluster analysis applying longest distance method based on LOX activity clusters all the varieties or lines into three major groups. There are great differences between the averages of LOX activity from varieties. LOX activities are discussed in the application of nutritional quality improvement in wheat.展开更多
The present study deals with some chemical behavior,including Cd extractability,species transformation and adsorption-desorption characteristics,of Cd in the rhizosphere(R) of wheat seedlings grown on both yellow-brow...The present study deals with some chemical behavior,including Cd extractability,species transformation and adsorption-desorption characteristics,of Cd in the rhizosphere(R) of wheat seedlings grown on both yellow-brown earth(YBE) and red earth (RE).Results indicated an increase of pH about 0.5 unit in the R of the yellow-brown earth and a decrease about 0.4 unit in the R of red earth in comparison with their respective bulk soils(NR).More Cd adsorbed and less Cd desorbed,and hence less Cd extracted in the R of YBE than those in the NR were found.The opposite cases were got for the R of RE.Fractionation experiment showed that in the R of YBE,more Cd was bound to Mn oxides and organic matter,less Cd was in exchangeable form,and little Cd was bound to amorphous and crystal iron oxides.However,little difference of the Cd species between the R and NR of RE was found. Regression analysis indicated that pH was one of the dominant factors affecting the chemical behavior of Cd in rhizosphere.展开更多
The aim of this work was to focus on development of the new health benefit food product, bread made from colored wheat flour with a high content of proteins, fibre, β-glucans, phenols, flavonoids. The amount of prote...The aim of this work was to focus on development of the new health benefit food product, bread made from colored wheat flour with a high content of proteins, fibre, β-glucans, phenols, flavonoids. The amount of protein ranged from 9% to 14% in our analyzed set. The highest value had yellow variety of wheat BONA DEA （14%）, at least had red grain of variety FEDERER （9.02%）. Average content of fl-glucans determined by enzymatically ranged from 0.25% （BONA VITA） to 0.67% （ABISSINSKAJA and LUTEUS）. The largest amount of phenolic compounds （134 mg GAE/L） and flavonoids （2.80 mg RE/L） contained sample RU440-6, the lowest sample was IS CORVINUS （87 mg GAE/L）. The highest antioxidant activity was observed in blue varieties of wheat 48M （0.269 mmol TE/L）, while the lowest was determined in a sample of purple ANK 28A （0.083 mmol TE/L）. Data show that there is potential to use purple, blue and red variety of wheat as novel ingredient resources for the development of new innovative products. Assessment of the results we selected two varieties of color wheat （KONINI, RU440-6） in bakery attempt to meet demand for nutritional quality and also have an increased content of healthy components. Overall the most pleasant taste, color and flexibility bread crumb had a loaf of bread prepared from a purple variety KONINI without the addition of β-glucans.展开更多
文摘A full_length cDNA has been cloned encoding nicotinamide adenine dinucleotide phosphate_specific glutamate dehydrogenase (NADP_GDH) from Chlorella sorokiniana with the RT_PCR method. The complete nucleotide sequence of NADP_GDH gene had 94% homology to the previously reported one . The NADP_GDH gene was constructed into a vector highly expressed in plants. The specific activity of NADP_GDH in transformants was detected, but not in the control plants. All transformed shoots on MS medium containing lower concentration of nitrogen and the transformed seedlings grown in lower concentration of nitrogen vermiculite had higher growth rate and more leaves than the control plants. Transformed leaf discs cultured on MS medium containing different nitrogen concentrations had more chlorophyll contents compared to the controls. These results suggested that exogenous NADP_GDH may enhance the absorption and utilization to ammonium in plants. The increased weight of transformed leaf discs cultured on medium supplemented with different concentrations of phosphinothricin (PPT) was more than that of control discs. 0.5 μg/mL PPT could be used as a selecting drug instead of kanamycin to develop the transformants. These results suggested that the NADP_GDH gene might be used as a new selecting gene in the future research of plant gene engineering.
基金Supported by the National Natural Science Foundation of China(NO:31371615)National Key Research&Developing Plan(2016YFD0300405)the National Natural Science Foundation of Anhui Province(1608085MC70)~~
文摘130 wheat varieties are selected as materials to test the LOX activity, whiteness, yellowness, carotenoids content and RVA parameters, which are to be analyzed by variance analysis, correlation analysis, cluster analysis and classified according to the origins. The result shows there are highly significant variations in LOX activity from varieties; LOX activity is positively correlated with whiteness, and significantly and negatively correlated with yellowness. Cluster analysis applying longest distance method based on LOX activity clusters all the varieties or lines into three major groups. There are great differences between the averages of LOX activity from varieties. LOX activities are discussed in the application of nutritional quality improvement in wheat.
文摘The present study deals with some chemical behavior,including Cd extractability,species transformation and adsorption-desorption characteristics,of Cd in the rhizosphere(R) of wheat seedlings grown on both yellow-brown earth(YBE) and red earth (RE).Results indicated an increase of pH about 0.5 unit in the R of the yellow-brown earth and a decrease about 0.4 unit in the R of red earth in comparison with their respective bulk soils(NR).More Cd adsorbed and less Cd desorbed,and hence less Cd extracted in the R of YBE than those in the NR were found.The opposite cases were got for the R of RE.Fractionation experiment showed that in the R of YBE,more Cd was bound to Mn oxides and organic matter,less Cd was in exchangeable form,and little Cd was bound to amorphous and crystal iron oxides.However,little difference of the Cd species between the R and NR of RE was found. Regression analysis indicated that pH was one of the dominant factors affecting the chemical behavior of Cd in rhizosphere.
文摘The aim of this work was to focus on development of the new health benefit food product, bread made from colored wheat flour with a high content of proteins, fibre, β-glucans, phenols, flavonoids. The amount of protein ranged from 9% to 14% in our analyzed set. The highest value had yellow variety of wheat BONA DEA （14%）, at least had red grain of variety FEDERER （9.02%）. Average content of fl-glucans determined by enzymatically ranged from 0.25% （BONA VITA） to 0.67% （ABISSINSKAJA and LUTEUS）. The largest amount of phenolic compounds （134 mg GAE/L） and flavonoids （2.80 mg RE/L） contained sample RU440-6, the lowest sample was IS CORVINUS （87 mg GAE/L）. The highest antioxidant activity was observed in blue varieties of wheat 48M （0.269 mmol TE/L）, while the lowest was determined in a sample of purple ANK 28A （0.083 mmol TE/L）. Data show that there is potential to use purple, blue and red variety of wheat as novel ingredient resources for the development of new innovative products. Assessment of the results we selected two varieties of color wheat （KONINI, RU440-6） in bakery attempt to meet demand for nutritional quality and also have an increased content of healthy components. Overall the most pleasant taste, color and flexibility bread crumb had a loaf of bread prepared from a purple variety KONINI without the addition of β-glucans.
