By solution culture experiment, three wheat genotypes (Ttiticum aestivum L.) and two oilseed rape genotypes (Brassica napus L.) differing in Mn efficiency under Mn-deficient conditions were used to study mechanisms of...By solution culture experiment, three wheat genotypes (Ttiticum aestivum L.) and two oilseed rape genotypes (Brassica napus L.) differing in Mn efficiency under Mn-deficient conditions were used to study mechanisms of the difference in Mn efficiency between wheat and oilseed rape. The results showed that there were significant differences in the abilities of MnIV reduction and acidification in root rhizosphere between the two species. Compared with wheat, oilseed rape had much higher reducing capacity and intensity of rhizosphere acidification under Mn-deficient conditions. Moreover, the higher ratio of functional leaves Mn/old leaves Mn in oilseed rape than in wheat was also an important factor for the different Mn efficiencies between the two species.展开更多
15-day old seedlings of wheat and rape were grown in a series of solutions with different concentrations of KNO3 for a definite period of time. The changes in NO3- concentration of the solutions were determined by the...15-day old seedlings of wheat and rape were grown in a series of solutions with different concentrations of KNO3 for a definite period of time. The changes in NO3- concentration of the solutions were determined by the double ion-selective electrode method, and then the amount of NO3- taken up by the plants was estimated and values of Km and Imax of the Michealis-Menten equation were calculated. Results show that both the method and conditions of determination affected the values of Km and Imax. For example, the Km value was appreciably reduced when the volume of culture solution was increased or when the duration of nutrient uptake was shortened; the Km value obtained with short-term depletion method was higher than that obtained with long-term one. Similar Variations were found for the values of Imax. There was a considerable difference in the characteristics of uptake kinetics between wheat and rape when determined under the same conditions of determination. The isotherm of NO3- uptake by wheat could be separated into saturated and unsaturated parts, and when the concentration of NO3- exceeded 180 uuuuuuuuuuuuM, the relationship between the rate of NO3- uptake and NO3- concentration tended to be linear. However, the isotherm of NO3- uptake by rape was found to fit the Michealis-Menten equation and no linear relationship could be found.展开更多
The positive effects of silicon(Si) on growth of plants have been well documented;however,the impact of Si on plant nutrient uptake remains unclear.The growth,nutrient content and uptake of wheat(Triticum aestivum L.)...The positive effects of silicon(Si) on growth of plants have been well documented;however,the impact of Si on plant nutrient uptake remains unclear.The growth,nutrient content and uptake of wheat(Triticum aestivum L.),canola(Brassica napus L.) and cotton(Gossypium hirsutum L.) plants were evaluated with or without application of 1.5 mmol L^(-1) Si.Application of Si increased dry weights by 8%,30%and 30%and relative growth rate(RGR) by 10%,13%and 17%in the cotton,canola and wheat plants,respectively.The plant relative water content(RWC) was also increased,but the plant transpiration was decreased by Si application.The uptake and content of Ca^(2+) were 19%and 21%lower in the cotton and wheat plants with Si than those without Si,respectively;however,Si application increased both K^+ and Fe uptake and contents in all plant species.Silicon application reduced B uptake and content only in cotton and increased P and Zn^(2+) contents in all three plant species.The decrease in Ca^(2+) uptake by Si application was sustained even in the presence of metabolic inhibitors 2,4-dinitrophenol and sodium cyanide.Uptake of Ca^(2+) by Si application was enhanced or did not change when plant shoots were saturated with water vapor or their roots were exposed to low temperature.Thus,Si application increased the uptake of transcellularly transported elements like K^+,P,Zn^(2+) and Fe.In contrast,Ca2+ uptake which occurred via both apoplastic and transcellular pathways was decreased by Si application,possibly through reduction of apoplastic uptake.More efficient nutrient uptake might be another promoting effect of Si on plant growth.展开更多
基金Project (No. 39425013) supported by the National Natural Science Foundation of China.
文摘By solution culture experiment, three wheat genotypes (Ttiticum aestivum L.) and two oilseed rape genotypes (Brassica napus L.) differing in Mn efficiency under Mn-deficient conditions were used to study mechanisms of the difference in Mn efficiency between wheat and oilseed rape. The results showed that there were significant differences in the abilities of MnIV reduction and acidification in root rhizosphere between the two species. Compared with wheat, oilseed rape had much higher reducing capacity and intensity of rhizosphere acidification under Mn-deficient conditions. Moreover, the higher ratio of functional leaves Mn/old leaves Mn in oilseed rape than in wheat was also an important factor for the different Mn efficiencies between the two species.
文摘15-day old seedlings of wheat and rape were grown in a series of solutions with different concentrations of KNO3 for a definite period of time. The changes in NO3- concentration of the solutions were determined by the double ion-selective electrode method, and then the amount of NO3- taken up by the plants was estimated and values of Km and Imax of the Michealis-Menten equation were calculated. Results show that both the method and conditions of determination affected the values of Km and Imax. For example, the Km value was appreciably reduced when the volume of culture solution was increased or when the duration of nutrient uptake was shortened; the Km value obtained with short-term depletion method was higher than that obtained with long-term one. Similar Variations were found for the values of Imax. There was a considerable difference in the characteristics of uptake kinetics between wheat and rape when determined under the same conditions of determination. The isotherm of NO3- uptake by wheat could be separated into saturated and unsaturated parts, and when the concentration of NO3- exceeded 180 uuuuuuuuuuuuM, the relationship between the rate of NO3- uptake and NO3- concentration tended to be linear. However, the isotherm of NO3- uptake by rape was found to fit the Michealis-Menten equation and no linear relationship could be found.
基金Golestan University Deputy of Research and Office of Higher Education,Iran,for financial support to Pooyan Mehrabanjoubani in the form of grants for Ph.D.research projects
文摘The positive effects of silicon(Si) on growth of plants have been well documented;however,the impact of Si on plant nutrient uptake remains unclear.The growth,nutrient content and uptake of wheat(Triticum aestivum L.),canola(Brassica napus L.) and cotton(Gossypium hirsutum L.) plants were evaluated with or without application of 1.5 mmol L^(-1) Si.Application of Si increased dry weights by 8%,30%and 30%and relative growth rate(RGR) by 10%,13%and 17%in the cotton,canola and wheat plants,respectively.The plant relative water content(RWC) was also increased,but the plant transpiration was decreased by Si application.The uptake and content of Ca^(2+) were 19%and 21%lower in the cotton and wheat plants with Si than those without Si,respectively;however,Si application increased both K^+ and Fe uptake and contents in all plant species.Silicon application reduced B uptake and content only in cotton and increased P and Zn^(2+) contents in all three plant species.The decrease in Ca^(2+) uptake by Si application was sustained even in the presence of metabolic inhibitors 2,4-dinitrophenol and sodium cyanide.Uptake of Ca^(2+) by Si application was enhanced or did not change when plant shoots were saturated with water vapor or their roots were exposed to low temperature.Thus,Si application increased the uptake of transcellularly transported elements like K^+,P,Zn^(2+) and Fe.In contrast,Ca2+ uptake which occurred via both apoplastic and transcellular pathways was decreased by Si application,possibly through reduction of apoplastic uptake.More efficient nutrient uptake might be another promoting effect of Si on plant growth.
