In nature, Apostichopus japonicus exhibits a behavioral characteristic of emerging at night and sheltering during the day. Hence, it was commonly believed that longer and darker light conditions are better for a popul...In nature, Apostichopus japonicus exhibits a behavioral characteristic of emerging at night and sheltering during the day. Hence, it was commonly believed that longer and darker light conditions are better for a population of A. japonicus. In aquaculture situations therefore, animals have commonly been cultured without lighting, especially during juvenile-hatching culture. However, how the length of darkness each day affects .4.japonicus and what the mechanism is of behavioral response of A. japonicus to different photoperiods remain unclear, and are specifically addressed in this study. We applied nine photoperiod treatments (light:dark (LD) cycles at 0:24, 3:21, 6:18, 9:15, 12:12, 15:9, 18:6, 21:3, and 24:0 under 500Ix) to 60 individuals (29.73±0.23 g) per treatment (with 3 replicates). The enviroramental conditions were set as: water temperature = 16.9±0.3℃, dissolved oxygen〉6.0 rag/L, ammonia〈0.3 mg/L, pH=7.8-8.2, and salinity=30-32. Under experimental conditions of continuous darkness as well as continuous light, cyclic nocturnal activity patterns of A. japonicus (viz. the animals emerged and fed at night and sheltered during the day) were observed. However, they spent more and less time, respectively, moving and feeding under continuous darkness and continuous light, than those under a natural light cycle. Under photoperiods with 6-12 h of light, the animals showed one sheltering behavior transition and two emerging behavior transitions. These behavior transitions appeared to be governed by some internal physiological factors and induced by daily light variation. The behavior of the animals was significantly affected by different photoperiods, and the distribution rate (DR) of the animals emerging at "daytime" increased with lengthening "light time" (except for the LD 3:21 cycle). We also found that there was no significant difference in mean DR per day under LD cycles of 6:18, 9:15, 15:9, and 12:12. Mean DRs per day were significantly lower for these photoperiods, than for those under LD cycles of 0:24 and 3:21, and significantly higher than for those under LD cycles of 18:6, 21:3 and 24:0. These results imply that 6-15 h light per day were the similar photoperiods to natural sunlight. From the viewpoint of activity and feeding behaviors, these might be the optimal photoperiods for rearing A. japonicus.展开更多
Objective: To study the clinical characteristics, treatment, and prognosis of thyroid cancer in children and adolescents. Methods: We performed a retrospective analysis of clinical data from 83 cases of thyroid canc...Objective: To study the clinical characteristics, treatment, and prognosis of thyroid cancer in children and adolescents. Methods: We performed a retrospective analysis of clinical data from 83 cases of thyroid cancer in children and adolescents from January 1990 to December 2010. We compared extra-thyroid extension, lymph node metastasis, distant metastasis, and prognosis between pediatric patients 〈12 years of age (27 cases) and those 〉 12 years of age (56 cases). All the patients agreed to undergo thyroidectomy and endocrine therapy, and the consent was obtained from parents or guardians. Results: Histopathology included papillary carcinoma in 67 cases, papillary carcinoma with partial follicular growth pattern in 1 case, papillary carcinoma with squamous metaplasia in 4 cases, follicular carcinoma in 7 cases, medullary carcinoma in 3 cases, and poorly differentiated carcinoma in 1 case. The total lymph node metastasis rate was 78.31%. Patients ≤12 years of age showed a higher rate of lymph node me- tastasis than the older group (92.59% vs. 71.43%, P=0.028). The incidence rate in females in the older group was higher than that in the younger group (80.36% vs. 59.26%, P=0.041). There were no significant differences in extra-thyroid extension, distant metastasis, survival rate, or recurrent disease between the two groups. Conclusions: The lymph node metastasis of thyroid cancer is higher in patients ≤12 years of age than in those 〉12 years of age; the incidence rate is higher in females than in males. Childhood thyroid cancer has a good prognosis, surgery being the most effective treatment. Choosing a reasonable surgery method and comprehensive postoperative treatment can achieve a cure and satisfactory survival rate.展开更多
Urbanization and exotic species are major threats to the conservation of forest-dependent wildlife species. Some emblematic species, indicators of habitat quality for the conservation of other spe- cies, might success...Urbanization and exotic species are major threats to the conservation of forest-dependent wildlife species. Some emblematic species, indicators of habitat quality for the conservation of other spe- cies, might successfully be reintroduced within cities when habitat restoration and pest manage- ment programs are combined. We studied the landscape resource selection of juvenile kaka Nestor meridionalis tracked with Global Positioning System (GPS) units and released into the predator- free reserve of Zealandia in Wellington city, New Zealand. Kaka moved beyond the predator exclu- sion fence into urban suburbs. The home range size and areas of high use estimated using local convex hull (a-LoCoH) ranged from 20 to 240 ha and 2 to 21 ha, respectively. Using resource selec- tion functions and model selection we found that native forest patches and urban areas close to the reserve were selected by kaka to establish their home ranges. At a lower scale of selection (i.e., se- lection of habitats within home ranges), kaka selected the same habitat, but not necessarily those close to the reserve. Native forest patches throughout the city can facilitate the dispersal of individ- uals, while the reserve provides protection and opportunities for supplementary feeding. Urban areas might have been selected due to the placement of feeders in private backyards. Survival of forest-dwelling species in cities requires careful urban planning and management to provide the necessary habitat patches, refugia, and food sources.展开更多
基金Supported by the National Natural Science Foundation of China (Nos. 30871931, 30771661)the National Key Technology Research and Development Program of China (Nos. 2006BAD09A01)the National Special Research Fund for Non-Profit Sector (No. 200905020)
文摘In nature, Apostichopus japonicus exhibits a behavioral characteristic of emerging at night and sheltering during the day. Hence, it was commonly believed that longer and darker light conditions are better for a population of A. japonicus. In aquaculture situations therefore, animals have commonly been cultured without lighting, especially during juvenile-hatching culture. However, how the length of darkness each day affects .4.japonicus and what the mechanism is of behavioral response of A. japonicus to different photoperiods remain unclear, and are specifically addressed in this study. We applied nine photoperiod treatments (light:dark (LD) cycles at 0:24, 3:21, 6:18, 9:15, 12:12, 15:9, 18:6, 21:3, and 24:0 under 500Ix) to 60 individuals (29.73±0.23 g) per treatment (with 3 replicates). The enviroramental conditions were set as: water temperature = 16.9±0.3℃, dissolved oxygen〉6.0 rag/L, ammonia〈0.3 mg/L, pH=7.8-8.2, and salinity=30-32. Under experimental conditions of continuous darkness as well as continuous light, cyclic nocturnal activity patterns of A. japonicus (viz. the animals emerged and fed at night and sheltered during the day) were observed. However, they spent more and less time, respectively, moving and feeding under continuous darkness and continuous light, than those under a natural light cycle. Under photoperiods with 6-12 h of light, the animals showed one sheltering behavior transition and two emerging behavior transitions. These behavior transitions appeared to be governed by some internal physiological factors and induced by daily light variation. The behavior of the animals was significantly affected by different photoperiods, and the distribution rate (DR) of the animals emerging at "daytime" increased with lengthening "light time" (except for the LD 3:21 cycle). We also found that there was no significant difference in mean DR per day under LD cycles of 6:18, 9:15, 15:9, and 12:12. Mean DRs per day were significantly lower for these photoperiods, than for those under LD cycles of 0:24 and 3:21, and significantly higher than for those under LD cycles of 18:6, 21:3 and 24:0. These results imply that 6-15 h light per day were the similar photoperiods to natural sunlight. From the viewpoint of activity and feeding behaviors, these might be the optimal photoperiods for rearing A. japonicus.
文摘Objective: To study the clinical characteristics, treatment, and prognosis of thyroid cancer in children and adolescents. Methods: We performed a retrospective analysis of clinical data from 83 cases of thyroid cancer in children and adolescents from January 1990 to December 2010. We compared extra-thyroid extension, lymph node metastasis, distant metastasis, and prognosis between pediatric patients 〈12 years of age (27 cases) and those 〉 12 years of age (56 cases). All the patients agreed to undergo thyroidectomy and endocrine therapy, and the consent was obtained from parents or guardians. Results: Histopathology included papillary carcinoma in 67 cases, papillary carcinoma with partial follicular growth pattern in 1 case, papillary carcinoma with squamous metaplasia in 4 cases, follicular carcinoma in 7 cases, medullary carcinoma in 3 cases, and poorly differentiated carcinoma in 1 case. The total lymph node metastasis rate was 78.31%. Patients ≤12 years of age showed a higher rate of lymph node me- tastasis than the older group (92.59% vs. 71.43%, P=0.028). The incidence rate in females in the older group was higher than that in the younger group (80.36% vs. 59.26%, P=0.041). There were no significant differences in extra-thyroid extension, distant metastasis, survival rate, or recurrent disease between the two groups. Conclusions: The lymph node metastasis of thyroid cancer is higher in patients ≤12 years of age than in those 〉12 years of age; the incidence rate is higher in females than in males. Childhood thyroid cancer has a good prognosis, surgery being the most effective treatment. Choosing a reasonable surgery method and comprehensive postoperative treatment can achieve a cure and satisfactory survival rate.
文摘Urbanization and exotic species are major threats to the conservation of forest-dependent wildlife species. Some emblematic species, indicators of habitat quality for the conservation of other spe- cies, might successfully be reintroduced within cities when habitat restoration and pest manage- ment programs are combined. We studied the landscape resource selection of juvenile kaka Nestor meridionalis tracked with Global Positioning System (GPS) units and released into the predator- free reserve of Zealandia in Wellington city, New Zealand. Kaka moved beyond the predator exclu- sion fence into urban suburbs. The home range size and areas of high use estimated using local convex hull (a-LoCoH) ranged from 20 to 240 ha and 2 to 21 ha, respectively. Using resource selec- tion functions and model selection we found that native forest patches and urban areas close to the reserve were selected by kaka to establish their home ranges. At a lower scale of selection (i.e., se- lection of habitats within home ranges), kaka selected the same habitat, but not necessarily those close to the reserve. Native forest patches throughout the city can facilitate the dispersal of individ- uals, while the reserve provides protection and opportunities for supplementary feeding. Urban areas might have been selected due to the placement of feeders in private backyards. Survival of forest-dwelling species in cities requires careful urban planning and management to provide the necessary habitat patches, refugia, and food sources.
基金the National Natural Science Foundation of China(No.61601410)the Zhejiang Provincial Natural Science Foundation of China(No.LY16F010018)the Science Foundation of Zhejiang Sci-Tech University(ZSTU)(No.15032085-Y)