期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
N-和C-末端组氨酸标记基因重组AxCeSD的柱层析分离特性 被引量:1
1
作者 胡松青 沈兴 +5 位作者 陈萍 姚闵 侯轶 高永贵 田中勳 李琳 《化工学报》 EI CAS CSCD 北大核心 2010年第1期99-103,共5页
Immobilization metal affinity chromatography(IMAC)and size-exclusive chromatography(SEC)have been widely used in the purification of recombinant protein.In order to apply the column chromatography to the separation an... Immobilization metal affinity chromatography(IMAC)and size-exclusive chromatography(SEC)have been widely used in the purification of recombinant protein.In order to apply the column chromatography to the separation and purification of the gene recombinant with histidine-tags,the column chromatographic separation characteristics of N-terminal histidine-tagged(N-AxCeSD)and C-terminal histidine-tagged(C-AxCeSD)gene recombinant protein AxCeSD,one of the subunit involved in the cellulose synthesis in Acetobacter xylinum were studied.In the ring-shaped three-dimensional structure of AxCeSD,N-terminal histidine-tags were located in the inner of ring,while C-terminal histidine-tags were located in the outer.A higher imidazole concentration was necessary for eluting the C-AxCeSD from the IMAC column due to the C-terminal histidine-tags had stronger chelating interaction with the Ni2+ on the IMAC media.Moreover,the retention time for eluting C-AxCeSD from the same SEC gel column was shorter than that for N-AxCeSD,because the larger protein homolog was formed in the C-AxCeSD solution through the inter-molecular hydrogen bonds between the C-terminal histidine-tags. 展开更多
关键词 组氨酸标记 固定化金属亲和层析 尺寸排阻层析 AxCeSD
下载PDF
Sephadex G-50纯化生姜蛋白酶的研究 被引量:3
2
作者 杜新永 唐晓珍 《食品工业科技》 CAS CSCD 北大核心 2010年第3期303-305,共3页
采用Sephadex G-50为填料对粗提生姜蛋白酶进行了纯化,充分利用了尺寸排阻层析简便、高效、重复性好的特点,以酶得率、纯化倍数、柱效分析等参数为指标,研究了凝胶柱床高度、洗脱速度、上样量等条件对纯化效果的影响。确定了最佳纯化方... 采用Sephadex G-50为填料对粗提生姜蛋白酶进行了纯化,充分利用了尺寸排阻层析简便、高效、重复性好的特点,以酶得率、纯化倍数、柱效分析等参数为指标,研究了凝胶柱床高度、洗脱速度、上样量等条件对纯化效果的影响。确定了最佳纯化方案为:柱床高度50cm,上样量3mL粗酶,流速45cm/h,洗脱液总用量150mL。纯化后的生姜蛋白酶比活性是姜汁的4.192倍,粗酶的2.113倍,而且还去除了色素、不溶物等杂质,纯化效果极佳。 展开更多
关键词 生姜蛋白酶 SEPHADEX G^50(葡聚糖凝胶) 尺寸排阻层析(SEC) 纯化
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部