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核化线粒体组学与转基因人分析法在人类进化研究中的应用1例
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作者 陈成立 周艳平 +2 位作者 张佳 廖端芳 李凯 《南华大学学报(医学版)》 2008年第6期806-807,共2页
5年前,作者提出转基因人分析法,即以人类染色体DNA与线粒体DNA的重组状况作为研究对象,并将这种研究线粒体DNA与染色体DNA之间相互作用的科学称之为核化线粒体组学。分析当时可利用的尼安特人的线粒体DNA高变区数百个碱基,认为不能排除... 5年前,作者提出转基因人分析法,即以人类染色体DNA与线粒体DNA的重组状况作为研究对象,并将这种研究线粒体DNA与染色体DNA之间相互作用的科学称之为核化线粒体组学。分析当时可利用的尼安特人的线粒体DNA高变区数百个碱基,认为不能排除尼安特人与现代人在基因水平上可能存在亲缘关系。最近,细胞杂志发表了尼安特人的线粒体DNA完全序列,通过利用核化线粒体组学方法,使5年前的推论得到了确认,即尼安特人与现代人在基因水平上存在亲缘关系。这一应用实例对分子考古学具有一定的参考价值。 展开更多
关键词 线粒体DNA 转基因人分析法 核化线粒体 进化 尼安特人
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Dionysos and Apollo: Remediating Said's Orientalism with Metacritical Perspective
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《Journal of Philosophy Study》 2017年第7期356-359,共4页
Geography is a kind of differential calculus in the sense that the three-dimensional, that is, the act of tangentially accessing things, is mapped on to the two-dimensional or the concrete. It is why we can say that t... Geography is a kind of differential calculus in the sense that the three-dimensional, that is, the act of tangentially accessing things, is mapped on to the two-dimensional or the concrete. It is why we can say that the East-West or Occidental versus Oriental dichotomy is so limited in its binary dualism. We could easily criticize not only Said's Orientalism, but also in turn, a critical self-defense by turning itself upon its own head. It can indeed be said that the cross or cardinal directions run four different ways and not two. "The East" is not just Far Eastern, that is, the so-called "Asian," but extends to the Far West or to California. Parts of Europe and the Dionysian are not simply limited to Central Europe and Southeastern Asia. We can see in Asia, that is, Eurasia and in North Africa, that 1-2% of non-Sub-saharan human DNA is genotypically Neanderthal in addition to being Homo Sapiens in DNA. 1 The task, it might be said, is to continually remediate binary directions and to reweave Apollo and Dionysos in Friedrich Nietzsche. We can see the limitations of Continentalism in categorizing the human. 展开更多
关键词 philosophical anthropology political geography Friedrich Nietzsche ORIENTALISM Edward Said metaphysical cartography
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Living fossils unearthed by blasting human chromosomes with Neanderthal mtDNA
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作者 ZHANG Jia ZHOU Cuilan +5 位作者 XIAO Li TUO Qinhui PENG Cuiying GUO Zifen LIAO Duanfang LI Kai 《Digital Chinese Medicine》 2022年第3期236-241,共6页
The successful retrieval of ancient mitochondrial DNA(mtDNA)from Neanderthals provides powerful experimental evidence that clarifies the arguments between the out-of-Africa and multiregional models of evolution.Howeve... The successful retrieval of ancient mitochondrial DNA(mtDNA)from Neanderthals provides powerful experimental evidence that clarifies the arguments between the out-of-Africa and multiregional models of evolution.However,the lack of nuclear DNA from Neanderthal fossils and mtDNA of early modern human fossils dating back to approximately the same time in the Pleistocene constitutes a limitation that may compromise the significance of mtDNA phylogenetic analysis.In this report,we introduce a mitochromic analysis using Neanderthal mtDNA as a foreign transgene and humans as a naturally occurring transgenic species.Forty Neanderthal mtDNA retrievable nuclear fragments were identified by blasting human genome data with Neanderthal mtDNA.Five of the 40 fragments exhibited higher correlation with Neanderthal mtDNA than those with modern human mtDNA.Furthermore,these five nuclear fragments harbor Neanderthal mtDNA-unique haplotypes.Based on the 98%+identity between Neanderthal and modern human mtDNA when compared by groups,we suggest that some of the modern human nuclear fragments retrieved using Neanderthal mtDNA may aid in decoding Neanderthal genetic information,and also may simultaneously demonstrate a close genetic evolutionary relationship between modern humans and Neanderthals. 展开更多
关键词 NEANDERTHAL Mitochromics Human evolution Mitochondrial DNA(mtDNA)
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Voltammetric Trace Determination of Prednisolone in Human Serum and Urine-Studies the Interaction with Albumin
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作者 Mohammed Ameen Abdullah Al-Imam Saddalah T. Sulaiman 《Journal of Chemistry and Chemical Engineering》 2013年第2期102-110,共9页
The Voltammetric properties of Pure Prednisolone in a direct method in the aqueous solution were studied. The substance has revealed a clear and major two reduction peaks at potential -1.22 Volt and -1.45 Volt against... The Voltammetric properties of Pure Prednisolone in a direct method in the aqueous solution were studied. The substance has revealed a clear and major two reduction peaks at potential -1.22 Volt and -1.45 Volt against the reference electrode (Ag/AgCI/SatKC1). The calibration curve of the prednisolone in the Phosphate Buffer (pH = 7) has been studies. The relationship has been linear within the scope of concentration 0.398 × 10 6-3.846 ×10-6 mol. The correlation coefficients are 0.9981, 0.9958 for the first and second peaks. Another two calibration curves were also constructed in the presence of human serum and urine. Standard addition method was successful used to determine the drug in serum, urine as well as in the analysis of drug in the tablet. The molecular binding ofprednisolone with Albumin was also studied at different temperature. The Vant hoff equation is used to calculate the thermodynamic parameters, the results indicate that the (ion-ion) of binding suggested. 展开更多
关键词 PREDNISOLONE VOLTAMMETRY interaction albumin.
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