AIM: To investigate the therapeutic effect of somatostatin receptor type 2 (SSTR2) gene transfection on pancreatic carcinoma xenografts in vivo in experimental cancers. METHODS: Human pancreatic cancer cell line Panc-...AIM: To investigate the therapeutic effect of somatostatin receptor type 2 (SSTR2) gene transfection on pancreatic carcinoma xenografts in vivo in experimental cancers. METHODS: Human pancreatic cancer cell line Panc-1 was inoculated subcutaneously into the back of nude mice. When tumor nodules were grown as large as about 5 mmx5 mm days after inoculation, the mice were randomly divided into 3 groups (6 mice in each group). Group Ⅰ served as untreated control group. Group Ⅱ received an intratumoral injection of a combination of human cytomegalovirus promoter-6C (pCMV-6C) and lipofectamine 2000. Group Ⅲ received an intratumoral injection of a combination of pCMV-6C-SSTR2 and lipofectamine 2000. The rate of tumor growth was compared among these three groups. The expression of SSTR2 in these tumors was detected by immunohistochemistry and Western-blot. Apoptosis index (AI) in these tumors was examined by using TUNEL in situ. RESULTS: Intratumoral injection of a combination of pCMV-6C-SSTR2 and lipofectamine 2000 resulted in the expression of SSTR2 protein. The tumor size and weight in group Ⅲ (0.318±0.098 cm3, and 0.523±0.090 g, respectively) were significantly lower than those in group I (2.058±0.176 cms, and 1.412±0.146 g, respectively) and group Ⅱ (2.025±0.163 cm3, and 1.365±0.116 g, respectively) (P<0.05) The AI in group Ⅲ (1.47±0.13%) was significantly higher than that in groupⅠ(0.56±0.09%) and group Ⅱ (0.57±0.11%) (P<0.05). But there were no significant differences between groups Ⅰ and Ⅱ. CONCLUSION: Our data demonstrate that re-expression of SSTR2 gene has antitumor effects on experimental pancreatic cancer. Restoration of SSTR2 gene expression through gene transfer in vivo might be a potential gene therapy strategy for human pancreatic cancer.展开更多
Total nitrogen (N) loss and ammonia volatilization from urea applied to flooded rice grown on a paddy soil in Zhejiang Province were measured by 15N balance and micro-meteorological methods, respectively. Floodwater p...Total nitrogen (N) loss and ammonia volatilization from urea applied to flooded rice grown on a paddy soil in Zhejiang Province were measured by 15N balance and micro-meteorological methods, respectively. Floodwater properties and ammonia loss from the circular plot were compared with those from the microplots. And the effectiveness of urease inhibitor, NBPT [N-(n-butyl) thiophosphonc triamide], was also tested Results showed that the total losses from urea broadcast and incorporated at transplanting (basal dressing) were similar with those from urea broadcast 12 days after transplanting (top-dressing) (51.5% and 48%, respectively, of applied N), and ammonia losses were low, the corresponding figures were 10.8% and 7.0% of applied N, respectively. Thus, denitrification was a much more important pathway of nitrogen loss than ammonia volatilization under the particular conditions. Addition of NBPT retarded urea hydrolysis, reduced pHs and ammoniacal nitrogen concentrations of floodwater for either the application of urea as basal or as top-dressing, but these effects were not translated into the reduction of total nitrogen loss. Floodwater pHs and ammonia loss in the microplots were apparently lower than those in the circular plot from urea applied as basal dressing; however, such differences were not found when urea was top-dressed. The frequently raining days occurred after top-dressing may be responsible for the insignificant effect of plot size on floodwater pHs and ammonia volatilization. It seems that the effects of plot size on floodwater properties and ammonia loss mainly depend on weather conditions, in addition to the height and leaf area index of the crop.展开更多
We evaluated the efficacy and tolerability of a dual therapy with rabeprazole and amoxicillin (AMX) as an empiric third-line rescue therapy. In patients with failure of first-line treatment with a proton pump inhibito...We evaluated the efficacy and tolerability of a dual therapy with rabeprazole and amoxicillin (AMX) as an empiric third-line rescue therapy. In patients with failure of first-line treatment with a proton pump inhibitor (PPI)- AMX-clarithromycin regimen and second-line treatment with the PPI-AMX-metronidazole regimen, a third-line eradication regimen with rabeprazole (10 mg q.i.d.) and AMX (500 mg q.i.d.) was prescribed for 2 wk. Eradication was confirmed by the results of the 13C-urea breath test (UBT) at 12 wk after the therapy. A total of 46 patients were included; however, two were lost to followup. The eradication rates as determined by per-protocol and intention-to-treat analyses were 65.9% and 63.0%,respectively. The pretreatment UBT results in the subjects showing eradication failure; those patients showing successful eradication comprised 32.9 ± 28.8 permil and 14.8 ± 12.8 permil, respectively. The pretreatment UBT results in the subjects with eradication failure were significantly higher than those in the patients with successful eradication (P = 0.019). A low pretreatment UBT result (≤ 28.5 permil) predicted the success of the eradication therapy with a positive predictive value of 81.3% and a sensitivity of 89.7%. Adverse effects were reported in 18.2% of the patients, mainly diarrhea and stomatitis. Dual therapy with rabeprazole and AMX appears to serve as a potential empirical third-line strategy for patients with low values on pretreatment UBT.展开更多
INTRODUCTION It is an interesting research area using urease inhibitors to curb urease activity in soil,to decrease the rate of urea hydrolysis and to increase the recovery of urea nitrogen(Zhou,1984;Guan,1985).Few st...INTRODUCTION It is an interesting research area using urease inhibitors to curb urease activity in soil,to decrease the rate of urea hydrolysis and to increase the recovery of urea nitrogen(Zhou,1984;Guan,1985).Few studies have been conducted on the relationship betweenthe effectiveness of the inhibitors applied to rice and the environmental conditions and展开更多
基金Supported by National Natural Science Foundation of China, No. 30271473
文摘AIM: To investigate the therapeutic effect of somatostatin receptor type 2 (SSTR2) gene transfection on pancreatic carcinoma xenografts in vivo in experimental cancers. METHODS: Human pancreatic cancer cell line Panc-1 was inoculated subcutaneously into the back of nude mice. When tumor nodules were grown as large as about 5 mmx5 mm days after inoculation, the mice were randomly divided into 3 groups (6 mice in each group). Group Ⅰ served as untreated control group. Group Ⅱ received an intratumoral injection of a combination of human cytomegalovirus promoter-6C (pCMV-6C) and lipofectamine 2000. Group Ⅲ received an intratumoral injection of a combination of pCMV-6C-SSTR2 and lipofectamine 2000. The rate of tumor growth was compared among these three groups. The expression of SSTR2 in these tumors was detected by immunohistochemistry and Western-blot. Apoptosis index (AI) in these tumors was examined by using TUNEL in situ. RESULTS: Intratumoral injection of a combination of pCMV-6C-SSTR2 and lipofectamine 2000 resulted in the expression of SSTR2 protein. The tumor size and weight in group Ⅲ (0.318±0.098 cm3, and 0.523±0.090 g, respectively) were significantly lower than those in group I (2.058±0.176 cms, and 1.412±0.146 g, respectively) and group Ⅱ (2.025±0.163 cm3, and 1.365±0.116 g, respectively) (P<0.05) The AI in group Ⅲ (1.47±0.13%) was significantly higher than that in groupⅠ(0.56±0.09%) and group Ⅱ (0.57±0.11%) (P<0.05). But there were no significant differences between groups Ⅰ and Ⅱ. CONCLUSION: Our data demonstrate that re-expression of SSTR2 gene has antitumor effects on experimental pancreatic cancer. Restoration of SSTR2 gene expression through gene transfer in vivo might be a potential gene therapy strategy for human pancreatic cancer.
文摘Total nitrogen (N) loss and ammonia volatilization from urea applied to flooded rice grown on a paddy soil in Zhejiang Province were measured by 15N balance and micro-meteorological methods, respectively. Floodwater properties and ammonia loss from the circular plot were compared with those from the microplots. And the effectiveness of urease inhibitor, NBPT [N-(n-butyl) thiophosphonc triamide], was also tested Results showed that the total losses from urea broadcast and incorporated at transplanting (basal dressing) were similar with those from urea broadcast 12 days after transplanting (top-dressing) (51.5% and 48%, respectively, of applied N), and ammonia losses were low, the corresponding figures were 10.8% and 7.0% of applied N, respectively. Thus, denitrification was a much more important pathway of nitrogen loss than ammonia volatilization under the particular conditions. Addition of NBPT retarded urea hydrolysis, reduced pHs and ammoniacal nitrogen concentrations of floodwater for either the application of urea as basal or as top-dressing, but these effects were not translated into the reduction of total nitrogen loss. Floodwater pHs and ammonia loss in the microplots were apparently lower than those in the circular plot from urea applied as basal dressing; however, such differences were not found when urea was top-dressed. The frequently raining days occurred after top-dressing may be responsible for the insignificant effect of plot size on floodwater pHs and ammonia volatilization. It seems that the effects of plot size on floodwater properties and ammonia loss mainly depend on weather conditions, in addition to the height and leaf area index of the crop.
基金Supported by A grant from the National Hospital Organization,No. H21-NHO-01
文摘We evaluated the efficacy and tolerability of a dual therapy with rabeprazole and amoxicillin (AMX) as an empiric third-line rescue therapy. In patients with failure of first-line treatment with a proton pump inhibitor (PPI)- AMX-clarithromycin regimen and second-line treatment with the PPI-AMX-metronidazole regimen, a third-line eradication regimen with rabeprazole (10 mg q.i.d.) and AMX (500 mg q.i.d.) was prescribed for 2 wk. Eradication was confirmed by the results of the 13C-urea breath test (UBT) at 12 wk after the therapy. A total of 46 patients were included; however, two were lost to followup. The eradication rates as determined by per-protocol and intention-to-treat analyses were 65.9% and 63.0%,respectively. The pretreatment UBT results in the subjects showing eradication failure; those patients showing successful eradication comprised 32.9 ± 28.8 permil and 14.8 ± 12.8 permil, respectively. The pretreatment UBT results in the subjects with eradication failure were significantly higher than those in the patients with successful eradication (P = 0.019). A low pretreatment UBT result (≤ 28.5 permil) predicted the success of the eradication therapy with a positive predictive value of 81.3% and a sensitivity of 89.7%. Adverse effects were reported in 18.2% of the patients, mainly diarrhea and stomatitis. Dual therapy with rabeprazole and AMX appears to serve as a potential empirical third-line strategy for patients with low values on pretreatment UBT.
文摘INTRODUCTION It is an interesting research area using urease inhibitors to curb urease activity in soil,to decrease the rate of urea hydrolysis and to increase the recovery of urea nitrogen(Zhou,1984;Guan,1985).Few studies have been conducted on the relationship betweenthe effectiveness of the inhibitors applied to rice and the environmental conditions and
