游泳池水中尿素检验方法的改进

<正>游泳池水中尿素含量测定方法常采用卫生部颁发的“二乙酰—肟比色定量法”(简称原法)。该法操作简便、灵敏度高,重现性好。但此法存在不足,对于尿素浓度高的水样,虽可少取水样稀释后(一般2~5倍)进行测定,但稀释倍数难于估计,所以需反复重测(尿素标准定为2.5mg/L,而该法最高浓度只能测到1.5mg/L),既费时,又浪费试剂。为此,本文在原法的基础上加以改进,这样,尿素含量高的水样亦可测定,改进后最高浓度可以测到4.0mg/L,超过4.0mg/L的水样进行一倍稀释基本上均在标准曲线范围以内,并保持原法的精密度与回收率。现报道如下。一、实验方法1．仪器与试剂同原法。保持原法酸度。2．操作步骤(1)取10ml或5ml水样(尿素含量在0.001~0.04mg)于25ml棕色具塞比色管中,另外在同类比色管中依次加入尿素标准溶液0.1~4.

对一种用于诊断幽门螺杆菌感染的新型快速尿素酶检验方法(Pronto Dry~)的前瞻性评价

Aim-Rapid urease tests are commonly used to establish the diagnosis of Helicobacter pylori infection during upper endoscopy.The aim of this study was to evaluate the performance of a new rapid urease test (Pronta Dry ) compared with histology as the gold standard. Methods-Six gastric biopsies (three in the antrum and three in the fundus) were performed in 113 consecutive patients. Eighteen patients were later excluded from analysis because they did not fulfil the inclusion criteria.Four biopsies were examined by two experienced pathologists blinded to the rapid urease tests. Two biopsies (one from antrum and one from the fundus) were pooled for the rapid urease test which was read by the endoscopist 5 and 30 minutes later using the color scale (yellow, pink, orange, dark pink, fuchsia) provided by the manufacturer. Results-According to the histology findings 32 of the 95 patients retained for analysis (33.7% )were positive for Helicobacter pylori. Considering that a positive test was indicated by the dark pink or fuchsia colors, sensitivity and specificity of Pronto Dry were 62.5% and 98.4% at 5 minutes and 84.4% and 98.4% at 30 minutes respectively. Twenty one of the 28 positive rapid urease tests (75% ) were already positive at 5 minutes. Conclusion-Considering positive tests are indicated solely by the two darkest colors on the color scale,the performance of Pronto Dry ) is similar to that of other rapid urease tests. The rapid results provided by Pronto Dry in routine proctice would seem to provide obvious advantages.

反相液相色谱法测定车用尿素水溶液中的缩二脲含量

与过去相比,人们的生活水平有了很大的提高,因此车用尿素的安全意识也在不断提高,对药品安全也提出了更高的要求。上世纪初,高效液相色谱技术开始出现,以其自身在色谱峰容量和灵敏度上的显著优势,极大地改变了检测、检测方式,被广泛应用于车用尿素检测。高效液相色谱法可以保证检测数据的准确性和检测结果的可靠性。本文综述了高效液相色谱法的原理和特点,同时分析了其在车用尿素检测中的具体应用,并对其新技术的发展和实际应用案例进行了详细探讨,以供参考。

化肥厂设备仪器预订单

为帮助各化肥生产企业完善生产和检测用设备仪器，认真贯彻实施相关标准，国家化肥质量监督检验中心(上海)与部分技术实力强、产品质量有保证的设备仪器生产厂家联系，可以提供以下设备仪器和技术，欢迎各化肥生产企业、检测机构、土肥站及科研部门来函来电预订。

^(14)C-urea breath test in patients undergoing anti-tuberculosis therapy

AIM: Urea breath test (UBT) is a non-invasive diagnostic test for detecting the presence of Helicobacter pylori(H pylori).In this study we evaluated the effect of anti-tuberculosis therapy on the results of 14C-UBT.METHODS: Patients, with the diagnosis of tuberculosis (TB) who had a positive UBT at the point of starting antiTB therapy, were included. None had a history of peptic ulcer disease or had taken antibiotics, bismuth compounds and/or PPI in the previous month, 14C-UBT was repeated at the end of the second month and the end of treatment period and one month after completion of treatment course.RESULTS: Thirty-five patients (23 males) were enrolled.14C-UBT was negative in all 35 patients (100%) at the end of the second month and remained negative in 30cases (85.7%) at the end of the treatment course. One month after completion of treatment course, UBT remained negative in 13 patients (37.1%).CONCLUSION: Our report underscores the need for caution while interpreting urea breath test results in patients undergoing anti-TB therapy. Furthermore, the combination of drugs used in this study resulted in H pylori eradication in a minority of patients.

Comparison of a monoclonal antigen stool test (Hp StAR) with the ^(13)C-urea breath test in monitoring Helicobacter pylori eradication therapy

AIM： To evaluate the agreement between a mAb-based stool test （HP STAR） and the urea breath test （UBT） in monitoring （H pylon） infection after eradication therapy. METHODS： Patients with discordant results on UBT and Hp STAR underwent endoscopy with biopsies for rapid urease test, culture, and histology to confirm H pylori status. RESULTS： Among 250 patients （50±14 years）, 240 （96.0%） had concordant UBT and Hp STAR tests with a significant correlation between DOB and A values （R = 0.87; P〈0.0001）. The remaining 10 （4.0%） patients had discordant tests （positive Hp STAR and negative UBT） with the Hp STAR inaccurate in five cases （false positive） and UBT inaccurate in the other five cases （false negative）. The “maximal expected” sensitivity, specificity, ＋PV, -PV, ＋LR, and -LR were 91%, 100%, 100%, 97.4%, ∞, and 8.2 respectively, for the UBT, and 100%, 97.4%, 91%, 100%, 38.8, and 0, respectively, for the Hp STAR. Overall accuracy for both tests was 98%. CONCLUSION： Both the UBT and the Hp StAR are equally accurate in monitoring H pylori infection. Nowadays, the choice of the “best” non-invasive H pylori test in the post-treatment setting should be done not only in terms of diagnostic accuracy but also in view of cost and local facilities.

提高尿素产品检验的准确度

尿素产品的检验依据，按照国家标准CB2440—2001《尿素及其测定方法》Urea and its determination严格执行，影响尿素产品优级品率的主要原因是缩二脲和水分含量。在生产车间从工艺、设备、操作方面不断优化，保证生产稳定运行的基础上，探讨如何通过准确的分析检验的全过程控制来提供准确数据，确保检验质量，使分析检验的准确率大于99％，及格率达到100％，从而不断提高尿素产品的优等品率。

One-year follow-up study of Helicobacter pylori eradication rate with ^(13)C-urea breath test after 3-d and 7-d rabeprazole-based triple therapy

AIM: To investigate the long-term role of a 3-d rabeprazole-based triple therapy in patients with Helicobacter pylori (H pylori)-infected active peptic ulcers. METHODS: We prospectively studied 115 consecutive patients with H pylori-infected active peptic ulcers. H pylori infection was confirmed if any two of H pylori DNA, histology, and rapid urease test were positive. Patients were assigned to either an open-labeled 3-d course of oral amoxicillin 1 000 mg b.i.d., clarithromycin 500 mg b.i.d., and rabeprazole 20 mg b.i.d., or 7-d course of oral amoxicillin 1 000 mg b.i.d., clarithromycin 500 mg b.i.d., and rabeprazole 20 mg b.i.d. Subsequently, all patients received oral rabeprazole 20 mg once daily until the 8th wk. Three months after therapy, all patients were followed-up endoscopically for the peptic ulcer, H pylori DNA, histology, and rapid urease test. One year after therapy, H pylori infection was tested using the 13C-urea breath test. RESULTS: The ulcer healing rates 3 mo after therapy were 81.0% vs 75.4% for the 3-d and 7-d groups [intention-to-treat (ITT) analysis, P=0.47] respectively, and 90.4% vs 89.6% for the 3-d and 7-d groups [per-protocol (PP) analysis, P=0.89] respectively. The eradication rates 3 mo after therapy were 75.9% vs 73.7% for the 3-d and 7-d groups (ITT, P=0.79) respectively, and 84.6% vs 87.5% for the 3-d and 7-d groups (PP, P=0.68) respectively. One year after therapy, seventy-five patients returned to receive the 13C-urea breath test, and the eradication rates were 78.4% vs 81.6% in 3-d and 7-d groups (PP, P=0.73) respectively. CONCLUSION: Our study showed the eradication rates against H pylori infection 3 and 12 mo after triple therapy were not different between the 3-d and 7-d rabeprazole-based groups. Therefore, the 3-d rabeprazole-based triple therapy may be an alternative treatment for peptic ulcers with H pylori infection.

分析碳14尿素呼气试验检测幽门螺旋杆菌感染的临床价值

探讨碳14尿素呼气试验应用在幽门螺杆菌感染检验中的临床价值。方法:以胃部疾病患者50例为研究对象,均取自我院2020年1月~2020年12月内收治患者,均需接受幽门螺杆菌感染检验,分别实施碳14尿素呼气试验检验和快速尿素酶检验,记录两种方式的检验结果,对比检验准确性、敏感度和特异性,分析临床应用价值。结果:比较两种方法检验阳性率,提示碳14尿素呼气试验72.00%(36/50)与快速尿素酶检验6200%(31/50)差异不高(P>0.05);碳14尿素呼气试验检验的准确性96.00%(48/50)、敏感度94.00%(47/50)、特异性88.00%(44/50)虽较快速尿素酶检验结果92.00%(46/50)、90.00%(45/50)、86.00%(43/50)高,但数据比较,差异无统计学意义(P>0.05)。结论:碳15尿素呼气试验对检验幽门螺杆菌感染具有积极作用,准确程度高,可以推广至临床工作。