This study aimed at acquiring knowledge on the hypoglycemic mechanisms of sodium metavanadate (SMV) showed that the liver glucokinase and muscle hexokinase activities increased rapidly after oral SMV was given, and th...This study aimed at acquiring knowledge on the hypoglycemic mechanisms of sodium metavanadate (SMV) showed that the liver glucokinase and muscle hexokinase activities increased rapidly after oral SMV was given, and that the blood glucose level was correlated closely with the activities of the two enzymes but not with the insulin level; which indicated that SMV could improve the altered glucose phosphorylation in diabetic mice independently of stimulating insulin secretion. This was probably one of the mechanisms of hypoglycemic effects of SMV.展开更多
AIM: To systematically investigate if cGMP/cGMP- dependent protein kinase G (PKG) signaling pathway may participate in dendroaspis natriuretic peptide (DNP)-induced relaxation of gastric circular smooth muscle. METHOD...AIM: To systematically investigate if cGMP/cGMP- dependent protein kinase G (PKG) signaling pathway may participate in dendroaspis natriuretic peptide (DNP)-induced relaxation of gastric circular smooth muscle. METHODS: The content of cGMP in guinea pig gastric antral smooth muscle tissue and perfusion solution were measured using radioimmunoassay; spontaneous contraction of gastric antral circular muscles recorded using a 4-channel physiograph; and Ca2+-activated K+ currents (IK(Ca)) and spontaneous transient outward currents (STOCs) in isolated gastric antral myocytes were recorded using the whole-cell patch clamp technique. RESULTS: DNP markedly enhanced cGMP levels in gastric antral smooth muscle tissue and in the perfusion medium. DNP induced relaxation in gastricantral circular smooth muscle, which was inhibited by KT5823, a cGMP-dependent PKG inhibitor. DNP increased IK(Ca). This effect was almost completely blocked by KT5823, and partially blocked by LY83583, an inhibitor of guanylate cyclase to change the production of cGMP. DNP also increased STOCs. The effect of DNP on STOCs was abolished in the presence of KT5823, but not affected by KT-5720, a PKA-specific inhibitor. CONCLUSION: DNP activates IK(Ca) and relaxes guinea-pig gastric antral circular smooth muscle via the cGMP/PKG-dependent singling axis instead of cAMP/ PKA pathway.展开更多
Objective： To observe the effect of moxibustion therapy plus cupping on perceived exertion and blood biochemical indicators in athletes with exercise-induced fatigue. Methods： A total of 30 gymnasts were randomized ...Objective： To observe the effect of moxibustion therapy plus cupping on perceived exertion and blood biochemical indicators in athletes with exercise-induced fatigue. Methods： A total of 30 gymnasts were randomized into two groups. After intensive physical training, 15 cases in the treatment group were treated by mild moxibustion and cupping, whereas the other 15 cases in the control group took a 30-rain break. After that, the Creatine Kinase （CK）, Blood Urea Nitrogen （BUN）, routine urinalysis, Rating of Perceived Exertion （RPE） and changes in Self-report Symptom Checklist 90 （SCL-90） were observed before and after one and periodic exercises. Results： After intensive physical training, athletes in the treatment group obtained a faster recovery of elevated CK levels than those in the control group. Analysis on the SCL-90 has shown that athletes in the treatment group had better interpersonal relations than those in the control group. Additionally, RPE analysis has also indicated that athletes in the treatment group had milder fatigue than those in the control group. Conclusion： Combined moxibustion with cupping is an effective and easy-to-operate method for exercise-induced fatigue.展开更多
文摘This study aimed at acquiring knowledge on the hypoglycemic mechanisms of sodium metavanadate (SMV) showed that the liver glucokinase and muscle hexokinase activities increased rapidly after oral SMV was given, and that the blood glucose level was correlated closely with the activities of the two enzymes but not with the insulin level; which indicated that SMV could improve the altered glucose phosphorylation in diabetic mice independently of stimulating insulin secretion. This was probably one of the mechanisms of hypoglycemic effects of SMV.
基金The National Natural Science Foundation of China, No. 30800382the Youth Science Foundation of Dalian to Professor Hui-Shu Guo, No. 2006B3NS218
文摘AIM: To systematically investigate if cGMP/cGMP- dependent protein kinase G (PKG) signaling pathway may participate in dendroaspis natriuretic peptide (DNP)-induced relaxation of gastric circular smooth muscle. METHODS: The content of cGMP in guinea pig gastric antral smooth muscle tissue and perfusion solution were measured using radioimmunoassay; spontaneous contraction of gastric antral circular muscles recorded using a 4-channel physiograph; and Ca2+-activated K+ currents (IK(Ca)) and spontaneous transient outward currents (STOCs) in isolated gastric antral myocytes were recorded using the whole-cell patch clamp technique. RESULTS: DNP markedly enhanced cGMP levels in gastric antral smooth muscle tissue and in the perfusion medium. DNP induced relaxation in gastricantral circular smooth muscle, which was inhibited by KT5823, a cGMP-dependent PKG inhibitor. DNP increased IK(Ca). This effect was almost completely blocked by KT5823, and partially blocked by LY83583, an inhibitor of guanylate cyclase to change the production of cGMP. DNP also increased STOCs. The effect of DNP on STOCs was abolished in the presence of KT5823, but not affected by KT-5720, a PKA-specific inhibitor. CONCLUSION: DNP activates IK(Ca) and relaxes guinea-pig gastric antral circular smooth muscle via the cGMP/PKG-dependent singling axis instead of cAMP/ PKA pathway.
基金supported by Shanghai Sports Bureau(04JT014)Shanghai Leading Academic Discipline Project(S30304)
文摘Objective： To observe the effect of moxibustion therapy plus cupping on perceived exertion and blood biochemical indicators in athletes with exercise-induced fatigue. Methods： A total of 30 gymnasts were randomized into two groups. After intensive physical training, 15 cases in the treatment group were treated by mild moxibustion and cupping, whereas the other 15 cases in the control group took a 30-rain break. After that, the Creatine Kinase （CK）, Blood Urea Nitrogen （BUN）, routine urinalysis, Rating of Perceived Exertion （RPE） and changes in Self-report Symptom Checklist 90 （SCL-90） were observed before and after one and periodic exercises. Results： After intensive physical training, athletes in the treatment group obtained a faster recovery of elevated CK levels than those in the control group. Analysis on the SCL-90 has shown that athletes in the treatment group had better interpersonal relations than those in the control group. Additionally, RPE analysis has also indicated that athletes in the treatment group had milder fatigue than those in the control group. Conclusion： Combined moxibustion with cupping is an effective and easy-to-operate method for exercise-induced fatigue.
