Diabetes mellitus is categorized as a major metabolic disease. Hyperglycemia is the populest mediator. The oxygen radicals＇ production or the peroxide production in the mitochondria is preceded as a possible mechanis...Diabetes mellitus is categorized as a major metabolic disease. Hyperglycemia is the populest mediator. The oxygen radicals＇ production or the peroxide production in the mitochondria is preceded as a possible mechanism. TGF-beta is characterized as the main protagonist. TGF-b is a molecular mediator with adherent and hypertrophic properties in diabetic nephropathy. 20 Balb/c male and female mice were used. They were separated in two age groups, one of 4 weeks old （childhood, n= 10） and one of 6 weeks old （puberty, n=10）. An immunochemical study for TGF-beta was undertaken. The basal petal of the external membrane of Bowmen is thickened. In significant percentage the basal membrane was thickened and the peduncles merge together. Mature sperm cells were not normal. The two age groups did not present significant differences in morphology of the exocrine pancreatic cells. The augmented levels of TGF-beta expression promote the increased proliferation of the pancreatic glandular cells, the derangement of differentiation and the apoptosis rhythm. The renal function is diminished sooner than the clinical manifestations. The testicles are less influenced concerning principally the spermiogenesis.展开更多
Objectives： The burn repair associated with diabetes complications showed considerable changes in the healing process and the development of alternative therapies to favor the repair is important. This study investig...Objectives： The burn repair associated with diabetes complications showed considerable changes in the healing process and the development of alternative therapies to favor the repair is important. This study investigated the efficacy of the Casearia sylvestris in the burn repair in diabetic and non-diabetic rats. Methods： The animals were divided into four groups （n = 20）： （C） non-diabetics treated with carbopol gel; （G） non-diabetic treated with C. sylvestris extract in carbopol gel; （DM-C） diabetics treated with carbopol gel; （DM-G） diabetics treated with C. sylvestris extract in carbopol gel. Burn was induced using a metal plate （2 cm diameter/120 °C/20 sec）. Samples were collected on the 3rd, 7th, 14th and 21st days after the injury for histomorphometric analysis （inflammatory infiltrate, fibroblasts, blood vessels and collagen）, MPO （Myeloperoxidase） and NAG （N-Acetilglicosaminidase） dosage, hydroxyproline, glycosaminoglycans, TGF-β1, VEGF, collagen I and III. Key findings： C. sylvestris decreased the inflammatory process during the experimental period in diabetic and non-diabetic animals which has been demonstrated by MPO, NAG quantification, and TGF-β1 expression. C. sylvestris also increased blood vessels and decreased VEGF expression during the studied period in non-diabetic animals. Fibroplasia and collagenase were increased in all experimental periods in the DM-G group by means of de hydroxyproline quantification, collagen I, collagen III and glycosaminoglycans. Conclusions： C. sylvestris application can modulate favorably the time of inflammation and collagenous process in the repair of burn injuries in diabetic rats.展开更多
文摘Diabetes mellitus is categorized as a major metabolic disease. Hyperglycemia is the populest mediator. The oxygen radicals＇ production or the peroxide production in the mitochondria is preceded as a possible mechanism. TGF-beta is characterized as the main protagonist. TGF-b is a molecular mediator with adherent and hypertrophic properties in diabetic nephropathy. 20 Balb/c male and female mice were used. They were separated in two age groups, one of 4 weeks old （childhood, n= 10） and one of 6 weeks old （puberty, n=10）. An immunochemical study for TGF-beta was undertaken. The basal petal of the external membrane of Bowmen is thickened. In significant percentage the basal membrane was thickened and the peduncles merge together. Mature sperm cells were not normal. The two age groups did not present significant differences in morphology of the exocrine pancreatic cells. The augmented levels of TGF-beta expression promote the increased proliferation of the pancreatic glandular cells, the derangement of differentiation and the apoptosis rhythm. The renal function is diminished sooner than the clinical manifestations. The testicles are less influenced concerning principally the spermiogenesis.
文摘Objectives： The burn repair associated with diabetes complications showed considerable changes in the healing process and the development of alternative therapies to favor the repair is important. This study investigated the efficacy of the Casearia sylvestris in the burn repair in diabetic and non-diabetic rats. Methods： The animals were divided into four groups （n = 20）： （C） non-diabetics treated with carbopol gel; （G） non-diabetic treated with C. sylvestris extract in carbopol gel; （DM-C） diabetics treated with carbopol gel; （DM-G） diabetics treated with C. sylvestris extract in carbopol gel. Burn was induced using a metal plate （2 cm diameter/120 °C/20 sec）. Samples were collected on the 3rd, 7th, 14th and 21st days after the injury for histomorphometric analysis （inflammatory infiltrate, fibroblasts, blood vessels and collagen）, MPO （Myeloperoxidase） and NAG （N-Acetilglicosaminidase） dosage, hydroxyproline, glycosaminoglycans, TGF-β1, VEGF, collagen I and III. Key findings： C. sylvestris decreased the inflammatory process during the experimental period in diabetic and non-diabetic animals which has been demonstrated by MPO, NAG quantification, and TGF-β1 expression. C. sylvestris also increased blood vessels and decreased VEGF expression during the studied period in non-diabetic animals. Fibroplasia and collagenase were increased in all experimental periods in the DM-G group by means of de hydroxyproline quantification, collagen I, collagen III and glycosaminoglycans. Conclusions： C. sylvestris application can modulate favorably the time of inflammation and collagenous process in the repair of burn injuries in diabetic rats.
