Aluminum pillared interlayered clay （Al-PILC） calcined at 773 K was the most appropriate support for immobilization of horseradish peroxidase （HRP） among several materials investigated.HRP could be immobilized on ...Aluminum pillared interlayered clay （Al-PILC） calcined at 773 K was the most appropriate support for immobilization of horseradish peroxidase （HRP） among several materials investigated.HRP could be immobilized on the Al-PILC via hydrogen bonding between functional groups of support and amino groups of the enzyme.The support and immobilized enzyme were characterized with N2-adsorption and desorption, XRD and FT-IR.An optimum condition for immobilization with enzyme immobilization efficiency of about 100% was obtained when the dose of support was greater than 0.45 mg per unit enzyme activity and reaction time exceeded 15 min.HRP immobilized on Al-PILC exhibited good catalytic performance.展开更多
以膨润土制备的层柱黏土为载体,考察给酶量、固定化pH、温度和时间对固定化β-葡萄糖醛酸苷酶活性的影响,并对其操作稳定性进行研究。结果表明:给酶量为2 700 U/g,最适pH为3.6,固定化温度40℃,固定化60 m in条件下固定化酶催化活性较高...以膨润土制备的层柱黏土为载体,考察给酶量、固定化pH、温度和时间对固定化β-葡萄糖醛酸苷酶活性的影响,并对其操作稳定性进行研究。结果表明:给酶量为2 700 U/g,最适pH为3.6,固定化温度40℃,固定化60 m in条件下固定化酶催化活性较高;酶经固定化后其热稳定性及储存稳定性显著提高。展开更多
文摘Aluminum pillared interlayered clay （Al-PILC） calcined at 773 K was the most appropriate support for immobilization of horseradish peroxidase （HRP） among several materials investigated.HRP could be immobilized on the Al-PILC via hydrogen bonding between functional groups of support and amino groups of the enzyme.The support and immobilized enzyme were characterized with N2-adsorption and desorption, XRD and FT-IR.An optimum condition for immobilization with enzyme immobilization efficiency of about 100% was obtained when the dose of support was greater than 0.45 mg per unit enzyme activity and reaction time exceeded 15 min.HRP immobilized on Al-PILC exhibited good catalytic performance.
