建立螺旋藻转基因体系初报

从钝顶螺旋藻单细胞克隆的制备、重组平台的 克隆、同源重组表达质粒的构建、质粒的电激转化和报告基因的表达等方面对螺旋藻转基因 表达系统进行了研究，初步建立起螺旋藻转基因体系。用次氯酸钠溶液处理获得无菌培养系 ；用钠、钙离子混合液处理，获得了可再生的螺旋藻单细胞。用含EDTA的培养基预培养和用 培养基洗涤可分别去除胞外核酸酶活性和抑制胞内核酸酶活性。以氯霉素乙酰转移酶基因替 换大肠杆菌表达质粒pBV220的抗性选择标记，同时插入系列同源重组片段和萤火虫荧光素酶 基因，构建了同源重组表达载体pBVCS01-04L。电激转化螺旋藻S6-4品系单 细胞，获得了具有稳定氯霉素抗性的培养系，并用SDS-聚丙烯酰胺凝胶电泳证实了报告基 因的表达。

建模样式:一种评估软件体系结构非功能属性的方法

软件体系结构设计是软件过程中最为重要的环节之一.在设计阶段完成对软件体系结构非功能属性的评估,对于高质量软件产品的开发非常重要.通过对统一建模语言(UML)的扩展,提出了“建模样式”用于在软件设计阶段对软件体系结构非功能属性进行评估,并结合“可用性链”建模样式在分析软件体系结构“单消息分发-多消息处理”可用性中的应用,给出了建模样式的使用示例.同时,针对建模样式的应用,还提出了“建模知识库”用于管理和维护建模样式,提供各建模样式中标签的参考值.基于UML的建模样式以及建模知识库的使用,可以简化对软件体系结构非功能属性评估的复杂度和工作量,使其可以为软件开发人员所用,并融入到高质量软件开发过程中.

Impact on Mountainous Agricultural Development in the Three Gorges Reservoir Area forced by Migrants of the Three Gorges Project

The Three Gorges Project attracts worldwide attention because of the Three Gorges migrants, and the agriculture of the Three Gorges Reservoir Area is a foundation for the development of the Three Gorges migrants. The Three Gorges Reservoir Area is situated at the mountainous area with bad agricultural development conditions and relatively low levels of development. As a result, the large-scale migration fias special influence on its agricultural development, which has attracted much attention. The paper analyzes influence that the migrants have forced on its agricultural development based on the scientific data, and makes some explorations on the models that are suitable for the development of mountainous agriculture in the Three Gorges Reservoir Area.

Development of City Transport System and Two-wheel Vehicles

Based on the study on the city transport systems of some typical cities worldwide,this paper put forward that each city transport system has its own development mode,which is influenced by the city development plan,economic development level,traveling vehicle composition etc..When some problems occur,such as the congestions caused by contradiction between the road capacity and vehicle composition,the city transport system may come into temporary maturity period.If the improvement for road system is limited meanwhile,optimized structure of vehicle composition should be an effective solution in this case.With the development of economy-internationalization,the development speed of city transport modernization is rapid.When traveling easiness is conflicting with efficiency,the advantages of public transport system become more obvious.Correspondingly,the superiority of two-wheel vehicles will reappear.Though the important function of two-wheel vehicles for alleviating city traffic problems is obvious,however,their development strategy must be reasonably proposed,and operation regulations must be performed accordingly.

Plan C: China’s Development under the Scarcity of Natural Capital

The critical issue of China’s modernization is whether it can free itself from the traditional modernization plan based on the relatively abundant natural capital, and innovatively create a developmental model of a large country under the scarcity of natural capital. This is why China is so keen on circular economy and economical use of resources. Focused on this issue, this paper summarizes the theoretical elements of the development under the scarcity of natural capital, points out that Plan C is the strategic choice for China’s future development, emphasizes that China needs to enhance the new industrialization, new urbanization and new modernization based on the restriction of natural capital, and discusses the technological and mechanistic support required to realize the development under the scarcity of natural capital.

ANALYSIS ON LAND-USE CHANGE AND SOCIO-ECONOMIC DRIVING FACTORS IN H AINAN ISLAND DURING 50YEARS FROM 1950TO 1999

Land-use change is an important part of global change research(IGBP,1990-1992).But the majority of researches focus on the natural dr iving force of land use change.In thi s paper,the authors studied the rela tionship be-tween socio-economic driving facto rs and land-use change with time series in Hainan Island.Based on the stud y for the evolution of administrative system and policies in Hainan Island and the comprehensive analysis on the socia l economy da-ta and changes of social policies,th e socio-economic policies,human activities and land use of Hainan can be divided into three periods with different ch aracters:period from1950to 1978ch aracterized by the planning economi c system,peri-od from 1978to 1988characterized by the transfer of economic system and a doption of open policy,new stage fro m 1988to 1998with high growing speed.On th e other hand the authors describe the character of the periods and land-use change process affected by socio-economic factors.This paper provides an important method for evaluating the development and changes of Hainan social economy dur ing the 50years from 1950to 1999and f orecasting the development of Hainan so-cial economy in future.

Ecotoudsm Model and Benefits of Periphery Regions in Western Sichuan Province

There are many problems in the minority regions of western Sichuan Province,such as serious soil erosion,fragile ecological environment,low levels of economy and so on.Ad- vantages of tourism resources in the"periphery regions"should be fully utilized,and"multi-tourism"meets the characteristics and present situation of the minority regions.The"multi-tourism de- velopment model"can exert the functions of tourism as the key industry.Based on the demands and the internal linkages among different industries,the model can accelerate the development of related-industries,such as eco-agriculture,eco-industry and so on. Through implementing the multi-tourism model,we can fully utilize the advantages of the periphery regions and promote the sustainable development of regional economy.

The Elite Strategies in the CIMS Subject of China 863 Program (1986-2000)

Manpower is the key factor to the success of the CIMS Subject of China 863 Program. Many manpower strategies have been used in the CIMS Subject, which include elite strategies, training and practicing strategies, and culture strategies. This paper is mainly about the elite strategies such as to discover the talents, to cultivate the elites and to establish the mechanism for the expert groups to work in team, echelon and vigor.

Sustainable Tourism Development Management of Local Cultural Landscapes

Nowa days,ecological and cultural tourism,especially those ancient villages and towns in China,have attracted an in- creasing number of tourists because of their unique cultural fea- tures.However,rapid development of tourism brings economic benefits,but results in a series of problems that threaten further tourism development and protection of ancient towns,such as over-commercialization and tourism urbanization.Taking Long- men ancient town as an example,this paper presented a sustain- able way of preserving and developing resources of ancient vil- lages and towns.First,identify cultural features and the extent of ancient villages and towns,and find the approach through which a cultural landscape zoning can be applied.Second,define a coop- erative function area to release the pressure on core areas and protect their authentic cultures by extending tourism space.Third, balance tourism development with traditional industries to en- hance the sustainability of community economies.

The Training and Culture Strategies in the CIMS Subject of China 863 Program(1986-2000)

Manpower is a key factor for the success of the CIMS Subject of China 863 Program. Many manpower strategies have been used in the CIMS Subject, which include elite strategies, training and practicing strategies, and culture strategies. This paper is mainly about the training and culture strategies such as to pay attention to the training and the practice of the technical team, and to form a fine CIMS culture.

Particle Swarm Optimization and Its Application in Transmission Network Expansion Planning

The author introduced particle swarm optimization as a new method for power transmission network expansion planning. A new discrete method for particle swarm optimization was developed,which is suitable for power transmission network expansion planning, and requires less computer s memory.The optimization fitness function construction, parameter selection, convergence judgement, and their characters were analyzod.Numerical simulation demonstrated the effectiveness and correctness or the method. This paper provides an academic and practical basis of particle swarm optimization in application of transmission network expansion planning for further investigation.

Research on Difference of Tourism Consumption Behavior of the Youth

The tourism consumption behavior of the young people has significant effect on the present social tourism consumption behavior. Intensifying the research on tourism consumption behavior of the youth group helps to explore the hot spot of tourism consumption, and designing specific marketing programs and taping into consumption potential is the key to market development. The research takes the university students in the youth group as an example to analyze the features and difference of tourism consumption behavior and observe tourism consumption marketing of the university students, for providing reference for the expansion of tourism market.

Regional Planning and Development Under the Maximization of Urban Agglomerative Economies

Starting from the meaning and types of urban agglomerative economies, with the analysis of the characteristicsand causes of urban agglomerative economies, this paper puts forward that the regional planning and development shouldattach importance to urban agglomerative economies, follow the law of the maximization of regional urban agglomerativeeconomies. It also points out the countermeasures and advices to facilitate the regional planning and development based on theprinciple.

Assessment of achievements of the Lima Climate Change Conference and perspectives on the future

The Lima call for climate action adopted at the Lima Climate Conference on Climate Change specifies that the principles of the United Nations Framework Convention on Climate Change,including the principle of common but differentiated responsibilities,shall apply to the new climate agreement to be adopted at the Paris Conference on Climate Change in 2015.Decisions on other heavily debated items,including the intended nationally determined contributions,were also made at the Lima Conference.The significant achievements in Lima and the positive momentum have laid a solid foundation for the adoption of a new climate agreement in the Paris Climate Conference.Four measures are proposed for China to meet great challenges in addressing climate change beyond 2020,including early formulation and issuance of a climate change law,establishment of a greenhouse gas emission trading scheme,promotion of advanced climate technology investments,and further international engagement for climate change.

Driving Smartization Through Intelligent Transport

Smart cities initiatives are developing all over the world, due to their support in favouring a better provision of services, in connection with the aim of achieving efficiency in cities management. More and more big corporations are interested in this topic to develop their businesses, as several issues are linked to these projects, like environment, water management, energy policies, education, culture, and so on. In literature these issues, known as drivers, were also related to sustainability aims which could be reached through the accomplishment of better performances within environment, economy, and society. This study synthesizes all the different drivers identified by scholars and industry players in a model, in order to use it as a framework to depict the linkages among the various smart cities projects. Since transport resulted as one of the most considered drivers in the analyzed contributions, the research questions were delineated to describe its role in the different phases of smart initiatives, and to understand if it could encourage and enable the other drivers＇ development. The multiple case study was chosen as the fittest methodological approach and it was conducted with the selection of three smart cities, one for each of the most cited platform models developed by industry players, which had started transport initiatives at least three years ago： Singapore （IBM）, Amsterdam （Accenture）, and San Francisco （Microsoft）. The analysis of the official documents set up by city departments and by the industry players showed transport as a driving force in smart cities projects which conveys a smart approach to different domains, especially energy savings, environment, and safety. The results also showed smart mobility as a pivot in stimulating investments and citizens＇ participation on different city drivers, especially as it concerns environment and services to both citizens and businesses. Smart transport initiatives, hardware and software infrastructures, technological devices, and people participation to smartization were closely interrelated in smart cities projects and they could be successfully used to achieve sustainability aims for different stakeholders.

China's Voluntary Mitigation Target and Road of Low-carbon Development

China is going through a rapid development stage of industrialization and urbanization.Although tremendous achievements have been made in the aspects of energy conservation,improvement of energy effectiveness and development of new and renewable energies,because of the rapid development of economy,it is difficult to change the huge total amount and fast increase of CO2 emission in the near future.China has to confront the tough challenge to address global climate change.China plans to reduce carbon intensity,that is,CO2 emissions per unit GDP,by 40 to 45% by 2020 compared with the 2005 level.It is a strategic option to coordinate domestic sustainable development with coping with global climate change on the basis of China's national circumstances,representing the core content and key measures for transforming development pattern and realizing low-carbon development.To achieve the target,more capital and technology inputs are required for energy conservation and low-carbon development during the twelfth and Thirteenth Five Year Plan period than in the Eleventh Five Year Plan period.In addition,energy conservation achieved by structural adjustment,industrial upgrading and product value-added improvement is also expected to play a greater role.Therefore,China should strengthen technological innovation,make greater efforts to transform the development pattern,take advantage of the synergistic effect of policies and measures while coping with global climate change and building a domestic tow-oriented society.China should also establish an industrial system characterized by low-carbon emission.Then China will ultimately achieve a win-win situation in both domestic sustainable development and coping with global climate change.

China's Electric Power Industry Achievements during the 9th Five-Year Plan Period and Development Prospects for the 10th Five-Year Plan Period

This paper looks back upon the achievements of the electric power industry during the Ninth Five-year Plan Period(1996-2000), and puts forward the developing targets and the working focuses for the Tenth Five-year Plan Period(2001-2005).

Application of Tradable Emission Permits in Europe

The purpose of this study is merely to review the current situation in the designing and implementation of the emission trading programs in Europe. Historical data show that although there is a series of shortcomings in their current functioning, employing such instruments for GHG reduction policy making is strongly expected to be efficient and effective. The European Union Emission Trading Scheme (EU ETS), Clean Development Mechanism (CDM) and Joint Implementation (JI) are just a few examples of the ambitious EU initiative that heavily relies on such instruments. We dwell on their operations and achievements by far and all the content in this article is expected to convince the Chinese government and regional public authorities to take positive actions and attitudes in promoting these instruments.

Meeting Engineering Program Objectives through Service Learning Opportunities in Developing Countries

The civil engineering profession, in an adaptive reaction to emerging roles for civil engineers, is recognizing the need for new engineers to possess a more robust skill set than just the typical design background. This paper describes the efforts of Clemson Engineers for Developing Countries （CEDC） to fulfill the more nontraditional and often unaddressed ＂learning outcomes＂ noted by ASCE＇s Civil Engineering Body of Knowledge lbr the 21st Century as important prerequisites for licensure. The learning outcomes are addressed through ongoing international service learning projects in Cange, Haiti. The paper focuses on the following four outcomes and their fulfillment methods： leadership, globalization, teamwork, and communications. The student led organization has allowed students to set up their own fundraising mechanisms, to seek out members to join design review boards, and to develop their own project objectives. This level of student autonomy is n,3ted as key to ensuring that students achieve competency in these four areas.

Is It a Vain Hope That Museum Children Education Applies to All Children？ --A Research on the Classification and Age-group Division of Children

The museum education focusing on the children is different from adult acceptors for the mental development of the children presents obvious stage features. Therefore, a clear definition on the ages of the children in the service must be made. The children in different ages can find their own educational project groups in a short time according to their own categories and ages. To facilitate the branding of routine childhood education programs, improve the implementing benefit of childhood education programs and realize the maximization of resource utilization in the Museum Children Education, this study firstly generalizes and extracts one of the common problems that affect the implementing benefit of Chinese Museum Children Education project based on the case analysis - focus and age-group of service objects. Then this paper （article） explores it step by step. Finally the article combines the analyses with the empirical researches on feedback information acquired in on-site observations and semi-structured interviews, to propose improvement suggestions for the problem.