By investigating the shear effect on submerged cultivation of a traditional Chinese medicinal herb Ganoderma lucidum, a relatively high cell concentration of 13.8 g·L-1 by dry mass was obtained in bioreactor at a...By investigating the shear effect on submerged cultivation of a traditional Chinese medicinal herb Ganoderma lucidum, a relatively high cell concentration of 13.8 g·L-1 by dry mass was obtained in bioreactor at an impeller tip speed (ITS) of 0.51m·s-1. At an ITS of 0.51,1.02 and 1.53m·s-1, a maximal production titer of intracellular polysaccharide was 2.64, 2.20 and 2.28g·L-1 and that of ganoderic acid was 306, 299 and 273g·L-1, respectively. Under these ITSs, the maximal mean projected area of dispersed hyphae was 3.70, 2.54 and 2.13×104μm2, and that of pellets was 0.91, 0.67 and 0.55mm2, respectively. The information obtained is useful for efficient submerged cultivation of mushrooms on a large scale.展开更多
In this work, an equilibrium-dispersion model was successfully established to describe the breakthrough performance of Ca（Ⅱ） imprinted chitosan （Ca（Ⅱ）-CS） microspheres packed column for metal adsorption, and t...In this work, an equilibrium-dispersion model was successfully established to describe the breakthrough performance of Ca（Ⅱ） imprinted chitosan （Ca（Ⅱ）-CS） microspheres packed column for metal adsorption, and the assumptions of Langmuir isotherms and axial dispersion controlled mass transfer process were confirmed. The axial dispersion coefficient in Ca（Ⅱ）-CS microspheres packed column was found to be almost proportional to the linear velocity and fit for prediction through single breakthrough test. Sensitivity analysis for breakthrough curve indicated the axial dispersion coefficient as well as Langmuir coefficient was sensitive variable for deep removal requirement. The retrieval of the adsorption isotherms of Ca（Ⅱ）-CS microspheres from breakthrough curve was fulfilled by modelling calibration. A strategy based on the correlation between adsorption isotherms and breakthrough performance was further proposed to simplify the column adsorption design using absorbents with small/uniform size and fast adsorption kinetics like Ca（Ⅱ）-CS microspheres to cut down the gap between lab and industry.展开更多
An optical platform for sensitive detection of chemical vapor based on a polymer-coated symmetrical metal-cladding waveguide is proposed.The diffusion of chemical vapor usually leads to a combinational effect in the p...An optical platform for sensitive detection of chemical vapor based on a polymer-coated symmetrical metal-cladding waveguide is proposed.The diffusion of chemical vapor usually leads to a combinational effect in the polymer layer,i.e.,swelling and refractive index change.Owing to the high sensitivity of ultrahigh-order modes,the vapor-induced effect will give rise to a dramatic variation of the reflected light intensity.For proof-of-concept,a good linearity and a low detection limit of toluene and benzene are experimentally demonstrated with an amorphous Teflon AF polymer layer.展开更多
基金This research was supported by the National Science Foundation(USA)project"Biotic Survey of the Gaoligongshan,a Biodiversity Hotspot in Western Yunnan,China"(DEB-0103795,Peter Fritsch,PI,David Kavanaugh,Nina Jablonski and the late Joe Slowinski,co-PI's) also partly supported theFoundation of Nature Sciences of the Education Department of Hunan Province and the National Natural Sciences Foundation of China(NSFC-30670234 ,30499341)
基金Supported by the National Natural Science Foundation of China (No. 20076011, No. 20236040 and No. 20225619).
文摘By investigating the shear effect on submerged cultivation of a traditional Chinese medicinal herb Ganoderma lucidum, a relatively high cell concentration of 13.8 g·L-1 by dry mass was obtained in bioreactor at an impeller tip speed (ITS) of 0.51m·s-1. At an ITS of 0.51,1.02 and 1.53m·s-1, a maximal production titer of intracellular polysaccharide was 2.64, 2.20 and 2.28g·L-1 and that of ganoderic acid was 306, 299 and 273g·L-1, respectively. Under these ITSs, the maximal mean projected area of dispersed hyphae was 3.70, 2.54 and 2.13×104μm2, and that of pellets was 0.91, 0.67 and 0.55mm2, respectively. The information obtained is useful for efficient submerged cultivation of mushrooms on a large scale.
基金the National Natural Science Foundation of China(2117613621422603)the National Science and Technology Support Program of China(2011BAC06B01)
文摘In this work, an equilibrium-dispersion model was successfully established to describe the breakthrough performance of Ca（Ⅱ） imprinted chitosan （Ca（Ⅱ）-CS） microspheres packed column for metal adsorption, and the assumptions of Langmuir isotherms and axial dispersion controlled mass transfer process were confirmed. The axial dispersion coefficient in Ca（Ⅱ）-CS microspheres packed column was found to be almost proportional to the linear velocity and fit for prediction through single breakthrough test. Sensitivity analysis for breakthrough curve indicated the axial dispersion coefficient as well as Langmuir coefficient was sensitive variable for deep removal requirement. The retrieval of the adsorption isotherms of Ca（Ⅱ）-CS microspheres from breakthrough curve was fulfilled by modelling calibration. A strategy based on the correlation between adsorption isotherms and breakthrough performance was further proposed to simplify the column adsorption design using absorbents with small/uniform size and fast adsorption kinetics like Ca（Ⅱ）-CS microspheres to cut down the gap between lab and industry.
基金supported by the National Natural Science Foundation of China (Grant No. 61168002)the Opening Foundation of the State Key Laboratory of Advanced Optical Communication Systems and Networks(Grant No. 2011GZKF031105)
文摘An optical platform for sensitive detection of chemical vapor based on a polymer-coated symmetrical metal-cladding waveguide is proposed.The diffusion of chemical vapor usually leads to a combinational effect in the polymer layer,i.e.,swelling and refractive index change.Owing to the high sensitivity of ultrahigh-order modes,the vapor-induced effect will give rise to a dramatic variation of the reflected light intensity.For proof-of-concept,a good linearity and a low detection limit of toluene and benzene are experimentally demonstrated with an amorphous Teflon AF polymer layer.
