厌氧消化污泥对退化苗圃土壤的改良效果研究

通过连续3年施用厌氧消化污泥(ADSS)的土柱试验,测定了土壤基本理化指标和微生物学指标,并分析了土壤中重金属积累特征,以全面评价厌氧消化污泥施用对合肥某退化苗圃土壤的改良效果.结果表明:施用厌氧消化污泥3年后,苗圃土壤质地和理化环境有明显改善,其中土壤中可溶性总盐含量(TDS)下降了67.6%,电导率(EC)降低了45.3%,对因多年种植苗木造成次生盐渍化的苗圃土壤具有较好的改良作用.施用厌氧消化污泥明显(概率P<0.05)增加了土壤中有机质和养分的供给,较无添加厌氧消化污泥相比,3年连续施用厌氧消化污泥后,土壤中有机质增加了77.9%;有效态氮和磷分别增加了110%和95.4%.同时,施用厌氧消化污泥对苗圃土壤微生物生物量和代谢活性也具有显著(P<0.05)的提升作用,其中微生物生物量碳(MBC)较无添加厌氧消化污泥增加了207%;尿酶和碱性磷酸酶活性分别增加了118%和102%.由此表明,施用厌氧消化污泥可以全面提升退化苗圃土壤质量.另外,通过对土壤中主要重金属含量分析表明,连续3年厌氧消化污泥施用后,苗圃土壤中重金属借有所增加,但其含量远低于土壤环境质量二级标准(GB 15618—1995),但是长期施用厌氧消化污泥后的土壤重金属累积特征和生态风险还有待进一步深入研究.

Effect of amendments on growth and metal uptake of giant reed(Arundo donax L.) grown on soil contaminated by arsenic,cadmium and lead

The effects of five amendments such as acetic acid（AA）, citric acid （CA）, ethylenediamine tetraacetic acid （EDTA）, sepiolite and phosphogypsum on growth and metal uptake of giant reed （Arundo donax L.） grown on soil contaminated by arsenic （As）, cadmium （Cd） and lead （Pb） were studied. The results showed that the shoot biomass of giant reed was enhanced by 24.8% and 15.0%, while superoxide mutase and catalase activities slightly varied when adding 5.0 mmol/kg CA and 2.5 mol/kg EDTA to soil as compared to the control, respectively. The concentrations of As, Cd and Pb in shoots were remarkably increased by the addition of 2.5 mmol/kg AA and CA, 5.0 mmol/kg EDTA, and 4.0 g/kg sepiolite as compared to the control. The accumulations of As and Cd were also significantly enhanced in the above condition, while the shoot Pb accumulation was noticeably enhanced by amending with 4.0 g/kg sepiolite and 8.0 g/kg phosphogysum, respectively. The results suggested that AA, CA and sepiolite could be used as optimum soil amendments for giant reed remediation system.

Differences of Uptake and Accumulation of Zinc in Four Species of Sedum

Four species of Sedum L. were grown in nutrient solution for the comparison of their Zn uptake and accumulation. S. alfredii Hance showed much greater tolerance to Zn than the other three species. Shoot and root yields of S. sarmentosum Bunge, S. bulbiferum Makino, and S. emarginatum Migo decreased with increasing Zn concentration in the solution, while shoot and root yields of S. alfredii increased when Zn concentration was ≤80 mg·L -1. At 80 mg·L -1 Zn, Zn concentration in shoots of S. alfredii reached 19.09 mg·g -1. S. alfredii was also more efficient in Zn translocation from roots to shoots, while Zn concentration in shoots was much higher than in roots. However, this was not the case for the other three species. The results showed that S. alfredii is a Zn hyperaccumulator and could be useful for the phytoremediation of Zn-contaminated soils.

Investigation on the Accumulation of Heavy Metals from Organic Fertilizer in Soil and Plant

ObjectiveThis study aimed to investigate the accumulation of heavy metals from organic fertilizer in soil and plant. MethodThree plots were chosen in Shanghai suburb to measure the heavy metal accumulation by monitoring their concentrations in soil and plant after organic fertilizer was applied. We also analyzed the correlations of the heavy metals in soil and plants. Single-factor pollution index and Nemerow’s synthetical pollution index were adopted to evaluate the heavy metal contamination in soils. Moreover, how many years before the heavy metal accumulation will exceed the environmental capability if 45 t/hm 2 organic fertilizer is applied every year was also estimated in the present study. ResultThe rules of heavy metals’ accumulation in soil changed with the various soil characters and pH. The average concentrations of Pb, Cd and As in the tested plants exceeded the limits. The average concentration of Cu in the tested soil shared positive correlation with that in the tested plants. The average concentration of Pb in the tested soil was negatively correlated with that in the tested plant while the other heavy metals didn’t show the rule like that. Organic fertilizer application caused no obvious pollution to the soils. Cu would exceed the standard environmental capacity within 15 years if 45 t/hm 2 organic fertilizer is applied every year, while for Hg, it will be 2 000 years. ConclusionWhen the excessive organic fertilizer is put into the land, the heavy metals from organic fertilizer would accumulate in soil and plant. With continued excessive fertilization, the heavy metals especially Cu would exceed the stan- dard environmental capacity. More attention should be paid to the inputting amount of the organic fertilizer.

Fractional distribution and risk assessment of heavy metal contaminated soil in vicinity of a lead/zinc mine

The pollution characteristics of Pb, Cd, Zn, Cu and Ni in soil of lead-zinc mining area were studied. The results indicate that the contamination degree followed the sequence of Cd〉Pb〉Zn〉Ni〉Cu and concentrations of Pb, Cd and Zn exceeded corresponding limits of the Chinese National Soil Environmental Quality Standard III. The soil was extremely polluted by Cd（Iego=5.26）, moderately to heavily polluted by Zn（Iego=2.38）, heavily to extremely polluted by Pb（Iego=4.13）. The results of BCR three-step sequential extraction procedure show that the active Cd, Pb and Zn were relatively high and might exert adverse effects on the plants grown in the soil, while Cu and Ni existed in soil with a relatively stable form. Potential ecological risk results indicate that soils were engaging in a high potential ecological risk by pollution of Cd and should be given rise to concern.

Toxicity and bioaccumulation of heavy metals in Phanerochaete chrysosporium

The responses of the growth and metabolism activity of Phanerochaete chrysosporium （P. chrysosporium） to cadmium （Cd）, lead （Pb） and their combined pollution stress, were investigated in plate and liquid culture conditions. The diameter of colony, biomass ofP. chrysosporium, ligninolytic enzyme activities and bioaccumulation quantity of heavy metals were detected. The results indicated that Cd was more toxic than Pb to P. chrysosporium and the toxicity of Cd and Pb to P. chrysosporium was further strengthened under Cd＋Pb combined pollution in different culture conditions. Heavy metals Cd and Pb had indirect influence on the production of ligninolytic enzymes by directly affecting the fungal growth and metabolic activity, and by another way in liquid culture. In addition, the results provided an evidence of the accumulation of Cd and Pb on the mycelia ofP. chrysosporium.

Effects of Ca^(2+)concentrations on accumulations of mineral elements in the components of Pteroceltis tatarinowii

The bark of Pteroce/tis tatarinowii is a raw material for manufacturing XuanPaper. The effects of Ca^(2+) concentrations on the accumulation of mineral elements in the bark,leaf and root of Pteroceltis tatarinowii were studied under controlled conditions. The types ofHoagland nutrient solution with three Ca^(2+) concentrations levels (200, 400 and 600 μg·g^(-1))and a control (without Ca^(2+)) were designed to culture Pteroceltis tatarinowii. After 6 months,contents of seven mineral elements including Ca, K, Mg, Mn, Zn, Cu and Na in the root, leaf and barkwere analyzed. The results indicated that Ca accumulations content in the root, leaf and bark hadpositively relation with Ca^(2+) concentrations (200, 400, 600 μg · g^(-1)), and the order of theCa content in the three components was root>leaf>bark. Ca content in the root treated with 600 μg·g^(-1) Ca^(2+) concentrations was 5.5 times as high as that of the control, and about 1.4 times ashigh as that of the root treated in 200 and 400 μg/g Ca^(2+) concentrations respectively. On thecontrary, K and Mg contents in the root, leaf and bark were negatively related to Ca^(2+)concentrations, especially in the bark, and their accumulation trend followed the order ofleaf>root>bark. K content in the bark treated with 600 μg ·g^(-1) Ca^(2+) concentrations was 39.3%of that of the control, and was 79.0% and 91.8% of that of the bark treated with 200 μg ·g^(-1)and 400 μg ·g^(-1) Ca^(2+) concentrations respectively; Mg content in the bark treated with 600μg ·g^(-1) Ca^(2+) concentrations was 23.4% of that of the control, and was 27.1% and 35.4% ofthat of the bark treated with 200 and 400 μg·g^(-1) Ca^(2+) concentrations respectively. Comparedwith the control, the general tendency of Mn, Zn and Cu content decreased with increasing of Ca^(2+)concentrations and their contents were in the order: root>leaf>bark. Based on the results of thisstudy, the experiment has been useful for providing academic bases in improving the bark quality ofPteroceltis tatarinowii on non-limestone soil.

Cultivation Ages Effect on Soil Physicochemical Properties and Heavy Metal Accumulation in Greenhouse Soils

The intensive management practices in greenhouse production may alter the soil physicochemical properties and contribute to the accumulation of heavy metals(HMs). To determine the HM concentrations in vegetable soil in relation to soil physicochemical properties and cultivation age, we conducted a soil survey for typical greenhouse soils in Shouguang, China. The results indicated that Cd is a major HM pollutant in the tested soils, as the only HM element exceeding the allowed limit for vegetable soil. The surveyed data was analyzed with regression analysis, correlation analysis and canonical correspondence analysis(CCA). A positive correlation is observed between HM pollution level and cultivation age. CCA results suggest that the HM pollution level and distribution in soil are significantly affected by soil physicochemical properties, which was a function of years of cultivation as revealed by regression analysis. In summary, cultivation age is an important factor to affect soil physicochemical properties(organic matter and inorganic nutrients) as well as HM contamination.

Accumulation of petroleum hydrocarbons and heavy metals in clams (Ruditapes philippinarum) in Jiaozhou Bay,China

Accumulation and distributions of aliphatic and polyaromatic hydrocarbons (PAHs) and heavy metals were measured in tissues of the clam Ruditapes philippinarum collected from 5 sites in Jiaozhou Bay,Qingdao,China.The concentrations of total aliphatic hydrocarbon and PAHs ranged from 570 to 2 574 ng/gdw (gram dry weight) and from 276 to 939 ng/gdw,in the most and least polluted sites,respectively.The bio-accumulation of hydrocarbons and PAHs in the clams appeared to be selective.Aliphatic hydrocarbons were predominantly represented by short chain (<nC23) n-alkanes,suggesting that petroleum hydrocarbons were likely the major contamination source.The selective uptake of 3 and 4 ring PAHs,such as naphthalene,fluorene,phenanthrene,fluoranthene and pyrene,by the clams was probably related to the physiological and bio-kinetic processes that were energetically favorable for uptake of compounds with fewer rings.Accumulation of the metals Cd,Cu,Zn,Pb,Cr,Hg,and As in the clam tissues also showed high variability,ranging from 0.043 to 87 μg/gdw.Among the 7 detected metals,Zn,Cd,Cu,and As had a particularly high potential of accumulation in R.philippinarum.In general,a positive correlation was found between the tissue concentrations and sediment concentrations of hydrocarbons and of some metals.Our study suggests that moderate contamination with polyaromatic hydrocarbons,and low to moderate contamination with metals,currently exists for clam R.philippinarum in Jiaozhou Bay,in comparison with other regional studies.A long-term monitoring program is certainly needed for assessment of the potential ecological influence and toxicity of these contaminants of R.philippinarum in Jiaozhou Bay.

Heavy Metal Bioaccumulation and Toxicity with Special Reference to Microalgae

The bioaccumulation and toxicity of heavy metals were reviewed with special reference to microalgae, the key compo- nent of the food web in aquatic ecosystems. Heavy metals enter algal cells either by means of active transport or by endocytosis through chelating proteins and affect various physiological and biochemical processes of the algae. The toxicity primarily results from their binding to the sulphydryl groups in proteins or disrupting protein structure or displacing essential elements. Metals can break the oxidative balance of the algae, inducing antioxidant enzymes, such as superoxide dismutase (SOD), glutathione peroxidase (GPX) and ascorbate peroxidase (APX). The amount of oxidized proteins and lipids in the algal cells thus indicates the severity of the stress. Algal tolerance to heavy metal is highly dependent upon the defense response against the probable oxidative damages. Pro- duction of binding factors and proteins, exclusion of metals from cells by ion-selective transporters and excretion or compartmen- talization have been suggested with regard to reducing heavy metal toxicity. However, a comprehensive description on the mecha- nisms underlining metal toxicity of microalgae and gaining tolerance is yet to be elaborated.

Effects of Waterborne Cu and Cd on Anti-oxidative Response, Lipid Peroxidation and Heavy Metals Accumulation in Abalone Haliotis discus hannai Ino

The aim of this study was to compare the effects of waterbome copper （Cu） and cadmium （Cd） on survival, anti-oxida- tive response, lipid peroxidation and metal accumulation in abalone Haliotis discus hannai. Experimental animals （initial weight： 7.49 g±0.01 g） were exposed to graded concentrations of waterborne Cu （0.02, 0.04, 0.06, 0.08 mg L-1） or Cd （0.025, 0.05, 0.25, 0.5 mgL-1） for 28 days, respectively. Activities of the anti-oxidative enzymes （catalase, CAT; superoxide dismutase, SOD; glutathione peroxidases, GPx; glutathione S-transferase, GST）, contents of the reduced glutathione （GSH） and malondiadehyde （MDA） in the hepatopancreas, and metal accumulation in hepatopancreas and muscles were analyzed after 0, 1, 3, 6, 10, 15, 21, 28 days of metal exposure, respectively. Results showed that 0.04 mg L-1, 0.06 mg L-1 and 0.08 mgL-1 Cu caused 100% death of abalone on the 21st, 10th and 6th day, respectively. However, no dead abalone was found during the 28-day waterborne Cd exposure at all experimental concentrations. Generally, activities of SOD and GST in hepatopancreas under all Cu concentrations followed a decrease trend as the exposure time prolonged. However, these activities were firstly increased and then decreased to the control level and increased again during Cd exposure. Activities of CAT in all Cu exposure treatments were higher than those in the control. These activities were firstly increased and then decreased to the control level and increased again during Cd exposure. Contents of MDA in hepatopancreas in all Cu treatments significantly increased first and then decreased to the control level. However, the MDA contents in hepatopan- creas were not significantly changed during the 28-day Cd exposure. The metals accumulation in both hepatopancreas and muscles of abalone significantly increased with the increase of waterborne metals concentration and exposure time. These results indicated that H. discus hannai has a positive anti-oxidative defense against Cu or Cd. In conclusion, anti-oxidative mechanism in abalone to resist waterborne Cu did not follow the same pattern as that for waterborne Cd.

Comparative transcriptomics reveals genes involved in metabolic and immune pathways in the digestive gland of scallop Chlamysfarreri following cadmium exposure

Chlamys farreri is an economically important mollusk that can accumulate excessive amounts of cadmium(Cd). Studying the molecular mechanism of Cd accumulation in bivalves is difficult because of the lack of genome background. Transcriptomic analysis based on high-throughput RNA sequencing has been shown to be an efficient and powerful method for the discovery of relevant genes in non-model and genome reference-free organisms. Here, we constructed two c DNA libraries(control and Cd exposure groups) from the digestive gland of C. farreri and compared the transcriptomic data between them. A total of 227 673 transcripts were assembled into 105 071 unigenes, most of which shared high similarity with sequences in the NCBI non-redundant protein database. For functional classification, 24 493 unigenes were assigned to Gene Ontology terms. Additionally, Eu Karyotic Ortholog Groups and Kyoto Encyclopedia of Genes and Genomes analyses assigned 12 028 unigenes to 26 categories and 7 849 unigenes to five pathways, respectively. Comparative transcriptomics analysis identified 3 800 unigenes that were differentially expressed in the Cd-treated group compared with the control group. Among them, genes associated with heavy metal accumulation were screened, including metallothionein, divalent metal transporter, and metal tolerance protein. The functional genes and predicted pathways identified in our study will contribute to a better understanding of the metabolic and immune system in the digestive gland of C. farreri. In addition, the transcriptomic data will provide a comprehensive resource that may contribute to the understanding of molecular mechanisms that respond to marine pollutants in bivalves.

Isolation of Mucor circinelloides Z4 and Mucor racemosus Z8 from heavy metal-contaminated soil and their potential in promoting phytoextraction with Guizhou oilseed rap

Fungi Z4 and Z8, isolated from the heavy metal polluted soil, have strong resistance to Cd and Pb. The strains were identified on the base of their morphology and internal transcribed spacers(ITS) region. Pot experiments were conducted to study the effect of two strains(Z4 and Z8) on the growth and accumulation of Cd and Pb of Guizhou oilseed rape. The results show that strains Z4 and Z8 belong to Mucor circinelloides and Mucor racemosus, respectively. The heights of Guizhou oilseed rape inoculated with strain Z8 increase by 47.90% than the control. The highest fresh mass is found in the plant with Z4/Z8, which is enhanced by160.81%. Pot experiments show that Z4/Z8 inoculums can accelerate accumulation of heavy metals in the plant. The contents of Cd and Pb are increased by 117.60% and 63.48%, respectively. Meanwhile, the heavy metal concentrations in potting soil with the two strains are found to be lower than those of the control, and the concentrations of Cd and Pb are decreased by 60.57% and 27.12%,respectively.

Heavy metals in sediments and their bioaccumulation in Phragmites australis in the Anzali wetland of Iran

Accumulation of metals in both sediments and Phragmites australis organs was studied. Samples were collected from seven stations located in Anzali wetland, Iran. The samples were analyzed by inductively coupled plasma mass spectrometry(ICP-MS). The results showed that concentration of the studied metals(except As and Cd) were higher in sediments than in P.australis organs. Metal accumulation was found to be signifi cantly(P <0.05) higher in roots than in above-ground organs of P.australis. The bioaccumulation factor(BAF) and the transfer factor(TF) also verifi ed the highest rate of metal accumulation in roots and their reduced mobility from roots to the above-ground organs. Pearson correlation coeffi cient showed signifi cant relationships between metal concentrations in sediments and those in plant organs. It should be pointed out that sediment and plant samples exhibited higher metal concentrations in eastern and central parts than in western and southern parts of the wetland. The mean concentrations of all studied elements(except for Fe, V and Al) were higher in these sediment samples than in the Earth's crust and shale. High accumulation of metals in P. australis organs(roots and shoots) is indicative of their high bioavailability in sediments of the wetland. The correlation between metal concentrations in sediments and in P. australis indicates that plant organs are good bioindicators of metal pollution in sediments of Anzali wetland.

Comparative Studies on Uptake Pathway of Cadmium by Perna viridis

Experiments were designed to expose the filter-feeding bivalve Perna viridis to different Cd-contaminated water environments in order to compare the different pathways through which Cd is accumulated. Results show that mussels can accumulate Cd through seawater, food, sediment and suspended particle pathways in a short period of time. Mussels＇ uptake of Cd through the seawater pathway reaches the highest concentration approximately 3 and 9 times larger than through the algae and sediment pathways respectively after 7 d. This indicates that the Cd-accumulation through seawater is most efficient. Results also indicate that the uptake directly through contaminated algae, particles or sediments ingested by mussels is less important when compared with the uptake of Cd by mussels through the seawater pathway. Metal uptake pathways and mechanisms of bioaccumulation by marine bivalve are also discussed in this paper.

Bioavailability of heavy metals in soil of the Tieguanyin tea garden, southeastern China

The bioavailability of 22 heavy metals was investigated at 19 sampling sites in Tieguanyin tea garden in Anxi County,Fujian Province,southeastern China.Heavy metal concentrations were determined by inductively coupled plasma-mass spectrometry(ICP-MS)and evaluated by geo-accumulation index(I_(geo)).Dilute nitric acid extraction was used to evaluate biological activity.Cu,Pb,and Cd were highly bioavailable and most easily absorbed by tea trees.Heavy metal bioavailability in the surface soil was as the ratio of the effective state to the total amount.Cd had the highest I_(geo)values,and the respective samples and sites were classified as moderately/strongly contaminated.Cd element is considered the main factor of heavy metal pollution in the tea garden in Anxi.The other heavy metals studied were present in lower concentrations;thus,the samples were classified as uncontaminated or slightly contaminated.

Analysis of Heavy Metal Content in Organic Fertilizers and Evaluation of Its Accumulation in Soil and Vegetable

The heavy metal contents of 54 organic fertilizer samples from Hunan Province were detected;meanwhile,through field plot experiments,the accumulations of heavy metals from chicken excrement-based organic fertilizer in soil and vegetables under different fertilization methods were studied.The experiment results indicated that:(1)The average contents of Pb,Cd,Cr,As,Hg,Cu,Zn and Ni in the sampled fertilizers were 8.18,0.77,17.80,2.80,0.83,70.36,198.84 and 81.48 mg/kg,respectively;Cu and Zn exceeded the most than their standards,followed by As,Hg,Cd and Cr.(2)Among the organic fertilizers produced by different raw materials,industrial sludge contained the highest content of heavy metals,followed by the livestock excrements,oil-seed-meals and crop straw.(3)Only applying chemical fertilizer had a relatively small in fluence on the accumulation of heavy metal in soil while decreasing its pH value;the mixture of chemical fertilizer and chicken manure could increase the pH value in soil while accumulating little heavy metals.It is recommended that the mixture of chemical fertilizer and chicken manure should be a prior choice so as to improve soil property and ensure the safety of agricultural products.Moreover,when applying double amount of chicken manure,the contents of 8 heavy metals in soil were increased,among which Cd,Cu,Zn had obtained obvious accumulation effects,which were higher than applying a single portion of chicken manure fertilizer.(4)After two years of the field plot experiments,the accumulation of heavy metal in vegetables was not obvious under all designed fertilization modes,though increasing slightly.

Distribution and Accumulation Characteristics of Heavy Metals in Sediments in Southern Sea Area of Huludao City, China

The southern sea area of the Huludao City, Liaoning Province might be polluted by heavy metals because it is close to the Jinzhou Bay, one of the heaviest sea area polluted by heavy metals in China. The undisturbed modem sediment core can be used to analyze the accumulation and source of the pollutants using 137Cs and 21pbex. Thirty-five samples of surface sediment and two core sedi- ments were collected from the southern sea area of Huludao City. The concentrations of copper （Cu）, lead （Pb）, chrome （Cr）, zinc （Zn）, arsenic （As） and mercury （Hg） in the surface sediments as well as Cu, Pb, Zn, Cr, 137Cs and 2~~pbex in the core sediments were deter- mined to research the spatial distribution and accumulation characteristics, and to analyze the sources and the potential risks of heavy metals. The results show that the pollution levels of Zn and Hg are serious, and 26 stations are at moderate or heavy ecological risks. The concentrations of the heavy metals increase from east to west, as well as from open sea to offshore marine area. The concentrations of heavy metals are not high in the sediments adjacent to the Jinzhou Bay, and the influence caused by the seawater exchange with the Jinzhou Bay is little. The concentrations of the heavy metals in the core sediments show low-high-low characteristic, and it coincides with the pollution history of Huludao City. The atmospheric deposition of heavy metals from the Huludao Zinc Plant is likely to be the main source of pollution without direct discharge of wastewater. The high concentrations of heavy metals appear on the upper sediment of 20 cm. The shallow sediment with high heavy metal contents might be exposed to surface when it was disturbed by the ocean engineering and big storm surge, then cause risk to the safety of aouaculture and human honlthy.

Enhanced mechanical properties of lamellar Cu/Al composites processed via high-temperature accumulative roll bonding

Cu/Al multilayers were produced by high-temperature accumulative roll bonding(ARB)methods up to three passes.To achieve a high bonding strength,prior to ARB processing,the Cu and Al sheets were heated to 350,400,450 and 500 ℃,respectively.The mechanical properties were evaluated by tensile tests.The microstructure was examined by optical microscopy and scanning electron microscopy equipped with energy dispersive spectrometry.The ultimate tensile stress,the grain size and the thickness of diffusion layer of lamellar composites increase with rolling temperature.When the rolling temperature is 400 ℃,the laminates show the highest ductility,but the yield stress is the lowest.As the rolling temperature further increases,both the yield stress and the ultimate tensile stress increase and the ductility decreases slightly.The mechanical properties of lamellar composites processed by low and high temperature ARB are determined by grain size and the thickness of diffusion layer,respectively.

Determination of Nutrient Uptake Characteristic of Black Pepper （Piper nigrum L.）

The relationship between the growth and nutrient uptake by perennial crop such as pepper is poorly understood and improved understanding of such relationship is important for the establishment of rational crop management practices. In order to characterize the growth performance and quantify the nutrient removed, this study presents results of three consecutive cropping years, fertilized with 1, 2 and 3 ton ha1 of NPK fertilizer respectively. Plant biomass accumulated was evaluated every two months, separating plant into stems, branches, leaves, berries, fruit spikes and flowers. Total biomass of pepper increased linearly and reach maximum at 22 months after planting. Thereafter, a decrease in dry matter was observed due to fruit export and fallen leaves at harvest. However, at the 28 months of planting, the biomass of pepper vine showing some increasing trend indicating the vegetative growth was reassumed for the next flowering. At 30 months, the pepper had removed 293.08 kg of nitrogen, 46.41 kg of phosphorus, 264.95 kg of potassium, 35.4 kg of magnesium and 74.82 kg of calcium. Based on data obtained, the nutrient uptake rates were lower than nutrient applied suggested that fertilizer had been overused for pepper production. In light of these results obtained, the optimum fertilizer dosage would be 62-10-62-6-18 kg/ha, 237-22-246-22-65 kg/ha and 390-62-352-47-100 kg/ha of N-P-K-Mg-Ca for the year 1, year 2 and year 3 of cropping year.