为确定沙门氏菌在肉牛屠宰过程中不同工序间传播路径与交叉污染情况,应用脉冲场凝胶电泳(pulsedfield gel electrophoresis,PFGE)技术对分离于肉牛屠宰过程6个工序点(粪便、皮毛、去皮后、喷淋后、排酸后、分割肉)的沙门氏菌进行分子溯...为确定沙门氏菌在肉牛屠宰过程中不同工序间传播路径与交叉污染情况,应用脉冲场凝胶电泳(pulsedfield gel electrophoresis,PFGE)技术对分离于肉牛屠宰过程6个工序点(粪便、皮毛、去皮后、喷淋后、排酸后、分割肉)的沙门氏菌进行分子溯源分析。结果证实,PFGE比血清分型具有更高的分辨率,48 株沙门氏菌的PFGE图谱相似度为48.0%~100.0%,所有菌株在95.7%的相似度下划分为22个基因型,不同工厂(地域)间未发现高度相似的基因型。宰前动物的皮毛和粪便间存在严重的交叉污染现象,部分来源于粪便、皮毛的沙门氏菌已经突破屠宰企业的防控屏障,对胴体(洁净区)造成了污染,具有进一步污染下游产品的可能性。因此,应加强宰前管理,减少宰前交叉污染对企业内部防控措施造成的压力,并对去皮后的胴体设置喷淋减菌的干预程序,以有效扼制沙门氏菌由动物胃肠道向屠宰线的传播。展开更多
For studying the germplasm charactedsUcs and carcass traits of Changtai yak, 8 adult yaks (5 male, 3 female) were selected and tested. The results showed that the pre-slaughter weight of the adult male and female Ch...For studying the germplasm charactedsUcs and carcass traits of Changtai yak, 8 adult yaks (5 male, 3 female) were selected and tested. The results showed that the pre-slaughter weight of the adult male and female Changtai yak was 364.32 and 266.83 kg, respectively, which presented extremely significant difference (P〈0.01). The carcass weight, net bone weight net meat weight, and the ratio of bone to meat also showed extremely significant difference (P〈O.01), which were 186.60 and 125.67 kg, 39.74 and 25.00 kg, 147.84 and 100.83 kg, 1:3.73 and 1: 4.03, respectively. The dressing percentage had significant difference which was 51.15% and 49.34%, respectively (P〈0.05). The neat meat percentage and carcass meat production rate showed no significant difference which were 40.54% and 37.66%, 79.29% and 80.24%, respectively (P〉0.05). It indicated that Changtai yak has better growth performance and meat performance potential, and worth of further studying and breeding.展开更多
文摘为确定沙门氏菌在肉牛屠宰过程中不同工序间传播路径与交叉污染情况,应用脉冲场凝胶电泳(pulsedfield gel electrophoresis,PFGE)技术对分离于肉牛屠宰过程6个工序点(粪便、皮毛、去皮后、喷淋后、排酸后、分割肉)的沙门氏菌进行分子溯源分析。结果证实,PFGE比血清分型具有更高的分辨率,48 株沙门氏菌的PFGE图谱相似度为48.0%~100.0%,所有菌株在95.7%的相似度下划分为22个基因型,不同工厂(地域)间未发现高度相似的基因型。宰前动物的皮毛和粪便间存在严重的交叉污染现象,部分来源于粪便、皮毛的沙门氏菌已经突破屠宰企业的防控屏障,对胴体(洁净区)造成了污染,具有进一步污染下游产品的可能性。因此,应加强宰前管理,减少宰前交叉污染对企业内部防控措施造成的压力,并对去皮后的胴体设置喷淋减菌的干预程序,以有效扼制沙门氏菌由动物胃肠道向屠宰线的传播。
基金Supported by Functional Gene Screen and Tissues' Differential Expression Analysis of Yak Muscle Development in Growth StagesNational Beef Cattle Industrial Technology System(CARS-38)+1 种基金Sichuan Province Yak Germplasm Resource Information Sharing Platform Construction Program(14010121)Ganzi Community Key Grass-Livestock Transformation Technology in Hongyuan County(201203008)~~
文摘For studying the germplasm charactedsUcs and carcass traits of Changtai yak, 8 adult yaks (5 male, 3 female) were selected and tested. The results showed that the pre-slaughter weight of the adult male and female Changtai yak was 364.32 and 266.83 kg, respectively, which presented extremely significant difference (P〈0.01). The carcass weight, net bone weight net meat weight, and the ratio of bone to meat also showed extremely significant difference (P〈O.01), which were 186.60 and 125.67 kg, 39.74 and 25.00 kg, 147.84 and 100.83 kg, 1:3.73 and 1: 4.03, respectively. The dressing percentage had significant difference which was 51.15% and 49.34%, respectively (P〈0.05). The neat meat percentage and carcass meat production rate showed no significant difference which were 40.54% and 37.66%, 79.29% and 80.24%, respectively (P〉0.05). It indicated that Changtai yak has better growth performance and meat performance potential, and worth of further studying and breeding.