A continued study on the bisoprolol and isosorbide dinitrate transdermal patches:cardiovascular protection in spontaneously hypertensive rats

The objective of the present study is to examine cardiovascular protective action of a newly developed transdermal patch by incorporating bisoprolol and isosorbide dinitrate in spontaneously hypertensive rats. As the combination therapy with these two synergistic drugs at low doses through a suitable form of administration could provide optimal therapeutic benefit, we further evaluated the effects of a 42 d period of anti-hypertensive treatment in spontaneously hypertensive rats. Rats were divided into the following five groups： control （blank patch）, bisoprolol fumarate tablets （BP-FT, 20.0 mg/kg, i.g.）, bisoprolol transdermal patch （BP-TP, 20.0 mg/kg）, isosorbide dinitrate transdermal patch （ISDN-TP, 20.0 mg/kg）, and the combination of BP and ISDN in a transdermal patch at low doses （8 and 12 mg/kg, respectively）. The effects of treatment were evaluated via biochemical indicators related to cardiovascular protection, structure and function. The combination therapy had synergistic anti-hypertensive effects and significantly reduced blood pressure with the benefit of controlling blood pressure variability compared to BP-FT and BP-TP. The combined treatment also reduced heart rate as well as BP-FT and BP-TP, while ISDN-TP had no evident effects on blood pressure, heart rate, and cardiovascular protection. Combination therapy was superior to BP-TP and BP-FT at increasing blood atrial natriuretic peptide and nitric oxide, while also reducing cardiac hydroxyproline and endothelin-1 with no difference in blood endothelin-1 and cardiac malondialdehyde levels. Cardiovascular remodeling differed among the groups, with the combination therapy reducing cardiac hypertrophy and the aortic media/lumen ratio. The consequential improvements in relaxation in response to cumulative concentrations of acetylcholine may explain the associated improvement in endothelial function. Combi- nation treatment with a transdermal patch exhibited a synergistic therapeutic effect. Such favorable cardiovascular effects with nitric oxide donors and β-blockade combination through a transdermal patch may provide long-term cardiovascular protection during anti-hypertensive treatment.

Molecular Composition and Evolution of the Chalcone Synthase (CHS) Gene Family in Five Species of Camellia (Theaceae)

The molecular composition and evolution of the chalcone synthase (CHS) gene family from five species in Camellia (Theaceae) are explored in this study. Sixteen CHS exon 2 from four Camellia species were amplified from total DNA by PCR method. Three sequences of the fifth species in Camellia and two sequences of Glycine max as the designated outgroups were obtained from GenBank. Our results indicated that CHS gene family in Camellia was differentiated to three subfamilies (A, B, C) during the evolutionary history with six groups (A1, A2, A3, BI, B2, C). Among them, only group A2 was possessed by all five species in this study. However, the other five groups were detected only in some species of the plants studied. All members of CHS gene family in this study had high sequence similarity, more than 90% among the members in the same subfamily and more than 78% among different subfamilies at nucleotide level., According to the estimated components of amino acids, the function of CHS genes in Camellia had been diverged. The nucleotide substitutions of the different groups were not identical. Based on phylogenetic analyse inferred from sequences of CHS genes and their deduced amino acid sequences, we concluded that the CHS genes with new function in this genus were evolved either by mutations on several important sites or by accumulation of the mutations after the gene duplication. A further analysis showed that the diversification of CHS genes in Camellia still occurred recently, and the evolutionary models were different to some extant among different species. So we assumed that the different evolutionary models resulted from the impacts of variable environmental elements after the events of speciation.

Genetic Polymorphism of Ten Microsatellites in Two Goat Breeds and Its Relationship with Heterosis

[Objective] This study aimed to investigate the genetic diversity of Macheng black goat and its correlation with heterosis.[Method] Ten microsatellite markers were selected for polymorphism investigation and statistical analysis of Boer goat and Macheng black goat populations.[Result] The results showed that totally 175 alleles were found in 10 microsatellite loci; to be specific,the maximum number of detected alleles was 23,and the minimum number was 10; the effective number of alleles （Ne） was 6.4-18.1,with absolute difference value of 1.6-8.1 from the observed number of alleles.The highest gene frequency was 0.239 1 and the lowest was 0.002 7.The polymorphic information contents of all the ten microsatellite markers were above 0.95.The observed heterozygosity （Ho） ranged from 0.616 7 to 0.984 4 and the expected heterozygosity （He） ranged from 0.844 1 to 0.944 6.The average expected heterozygosity of Boer goat and Macheng black goat was respectively 0.894 0 and 0.906 7.Various body weight and body size indices of Boer goatxMacheng black goat hybrids were improved in varying degrees compared with Macheng black goat （with an increase range of 0.32％-30.06％）.The average heterosis rates of body height and chest girth were relatively high,while average heterosis rate of body weight was relatively low.[Conclusion] The genetic distance between Boer goat and Macheng black goat was 0.379 5,which is consistent with the geographical distribution of Boer goat and Macheng black goat populations and is fully relevant to the heterosis of Boer goat × Macheng black goat hybrids,indicating that investigating polymorphism via microsatellite loci is one of the feasible means to predict and analyze heterosis between varieties.

Distribution of the Ibex (Capra ibex) in Tomur National Nature Reserve of Xinjiang, China

lbex （Capra ibex）, a first grade protected animal, has not been well studied in China. Surveys were conducted to investigate the distribution of the ibex in the Tomur National Nature Reserve during October--November 2004, July 2005, and October-December 2005. The results were as follows： （1） Out of five valleys, the ibex was distributed in the Qiong-Tailan, Keqik-Tailan, and Muzart Valleys： （2） Ibexes were not observed in the Tomur Valley and Kuzbayi Valley in our surveys, but interview investigations indicated that they may be found here as well. Our results indicated that the nature reserve as a whole is in the distribution range of ibex and it plays an important role in protecting ibex and the alpine biodiversity. However, the uneven distribution pattern can be due to human activities. Therefore, the management of the reserve needs to be improved in the future.

全国水利水电系统水利类职工大学专业协作组召开第二次协作会议

全国水利水电系统水利类职工大学专业协作组第二次协作会议于1985年9月17日至22日在山东省泰安县召开。我校派出两名代表参加。 1986年8月下旬水利水电部于牡丹江市召开的全国水利电力系统职工大学工作会议上决定成立各类职工大学的专业作组。

欢迎订阅《农村合作基金会概论》

为了指导农村合作基金会的工作,山东省农业厅经管处组织编写了《农村合作基金会概论》一书,已由山东省人民出版社出版。

新见傅山批注《尔雅注疏》简论

傅山是明末清初著名学者,他涉猎极广,著述甚丰,批注了诸多经书、子书。北京师范大学图书馆藏有一部傅山批注本《尔雅注疏》,此书未被收入《傅山全书》及其《补编》中,因此极具文献和学术价值。本文从版本选择、批注类型、治学精神等三个方面进行分析研究。

养好通往幸福的路

截至2016年年底,我国农村公路总里程已达396万公里。保护好来之不易的建设成果,养护是关键。现场会上,李小鹏部长讲话指出,全国"四好农村路"的工作目标是,到2020年基本建成结构科学合理的农村公路网。

发展“菜篮子”要有新思路

从近期召开的山东省"菜篮子"工作会议上获悉,"菜篮子"工程"九五"期间的任务是抓总量平衡,保障有效供应,而"十五"的任务则是在增加供应的基础上,进一步增加品种,优化产业结构,

高效创汇保健蔬菜——芦笋

芦笋又名石刁柏,被誉为世界十大名菜之一,具有极高的营养和保健价值。尤其是它的治癌防癌作用,早已被世界医学组织所公认,它还对治疗心血管疾病、降血压、降血脂、利尿效果明显。是一种药用价值极高的高效创汇保健蔬菜。

超载酿祸 冷藏车起火焚毁

3月10日清晨6时许。

《侦讯谋略》出版

《侦讯谋略》是山东省法学重点科研项目,由山东公安专科学校副校长、山东省法制心理学研究会会长庞兴华副教授编著、警官教育出版社1992年6月出版。全书30多万字,分为总论、侦察谋略、审讯谋略三篇十七章。该书是我国第一部专门论述侦察审讯谋略的专著,它以马列主义为指导,博采古今中外侦讯罪案的成功与失败的案例,总结我国同犯罪作斗争的丰富经验,吸收国内外最新科研成果。

辛亥革命前后青海反清起义略事

"清不清时天地昏,滚滚风尘神州愤;山东烟熄走铁板,河源薪燃现龙身。丹噶谋举黄表会,乔寿山敢当首领;虽然头悬城墙门,曾喊日月分半轮。"这首诗是丹噶尔黄表会和元山尔反清斗争始末的真实反映。清光绪末年,丹噶尔(湟源县)

Genetic Variation and Differentiation of Larix decidua Populations in Swiss Alps

Genetic diversity within and among six subpopulations of Larix decidua Mill. from two altitudinal transects of Swiss Alps was investigated using 6 enzyme systems coding for 8 loci. Globally, the mean proportion of polymorphic loci was 22.9%, the average number of alleles per locus was 1.3, and the mean expected heterozygosity was 0.095. Only 5.8% of the genetic variation resided among populations. The mean genetic distance was 0.006. Several significant differences of gene frequencies were found between different age classes. Positive values of the species mean fixation index observed in this study suggested a considerable deficit of heterozygotes in the populations of L. decidua of Swiss Alps. At one of the sites (Arpette), the highest subpopulation in elevation gave the lowest level of genetic diversity (as evidenced by the lowest proportion of polymorphic loci and the lowest mean expected heterozygosity) and the largest value of genetic distance when compared to other subpopulations. The genetic differences between the highest subpopulation and the other ones suggest that the founder effect may be an important factor influencing genetic differentiation of L. decidua populations at Arpette transect.

Mass Loss from Glaciers in the Chinese Altai Mountains between 1959 and 2008 Revealed Based on Historical Maps, SRTM, and ASTER Images

Mass loss of glaciers in the Chinese Altai was detected using geodetic methods based on topographical maps(1959), the Shuttle Radar Topography Mission(SRTM) Digital Elevation Model(DEM)(2000), and the Advanced Space-borne Thermal Emission and Reflection Radiometer(ASTER) stereo images(2008). The results indicate that a continued and accelerating shrinkage has occurred in the Chinese Altai Mountains during the last 50 years, with mass deficits of 0.43 ± 0.02 and0.54 ± 0.13 m a-1 water equivalent(w.e.) during the periods 1959-1999 and 1999-2008, respectively.Overall, the Chinese Altai Mountains have lost 7.06 ±0.44 km3 in ice volume(equivalent to-0.43 ± 0.03 m a-1 w.e.) from 1959-2008. The spatial heterogeneity in mass loss was potentially affected by comprehensive changes in temperature and precipitation, and had a substantial correlation withglacier size and topographic settings. Comparison shows that in the Chinese Altai Mountains glaciers have experienced a more rapid mass loss than those in the Tianshan and northwestern Tibetan Plateau(TP), and the mass balance of glaciers was slightly less negative relative to those in the Russian Altai, Himalaya, and southern TP.

Maintenance and development of the Ural high and its contribution to severe cold wave activities in winter 2020/21

Two successive severe cold waves invaded eastern China from the end of 2020 to early 2021,leading to an extensive,severe,and persistent drop in temperature.The paper investigates the features and formation mechanisms of the two cold waves.The main results are as follows:(1)An anticlockwise turning of the transverse trough was observed in both cold waves.However,a broad ridge was maintained over the Ural area from mid-December 2020 till mid-January 2021.No breakdown or discontinuous westward shift of the blocking high was observed,which is different from typical cold waves in eastern Asia.(2)The maintenance and strengthening of northerly winds in front of the Ural high led to an increase in baroclinicity in-situ.In the downstream region,the gradient of the geopotential height contour in the south of the transverse trough rapidly increased and the advection of cold temperature consistently enhanced and advanced southwards.This in turn caused the intensification and southward expansion of the Siberian high.(3)Energy propagation of the quasi-stationary wave was a reason for the development and persistence of the Ural blocking.Prior to the occurrence of the two cold waves,the energy of the low-frequency stationary wave originating from near 0°E(or even to the west)propagated eastwards,which helped the Ural ridge intensify and maintain.Meanwhile,it also contributed to the development of the trough downstream of the ridge and resulted in the anticlockwise turning of the transverse trough,providing a favorable condition for the southward outbreak of cold air.

Coupled 2D Hydrodynamic and Sediment Transport Modeling of Megaflood due to Glacier Dam-break in Altai Mountains,Southern Siberia

One of the largest known megafloods on earth resulted from a glacier dam-break,which occurred during the Late Quaternary in the Altai Mountains in Southern Siberia.Computational modeling is one of the viable approaches to enhancing the understanding of the flood events.The computational domain of this flood is over 9460 km2 and about 3.784 × 106 cells are involved as a 50 m × 50 m mesh is used,which necessitates a computationally efficient model.Here the Open MP(Open Multiprocessing) technique is adopted to parallelize the code of a coupled 2D hydrodynamic and sediment transport model.It is shown that the computational efficiency is enhanced by over 80% due to the parallelization.The floods over both fixed and mobile beds are well reproduced with specified discharge hydrographs at the dam site.Qualitatively,backwater effects during the flood are resolved at the bifurcation between the Chuja and Katun rivers.Quantitatively,the computed maximum stage and thalweg are physically consistent with the field data of the bars and deposits.The effects of sediment transport and morphological evolution on the flood are considerable.Sensitivity analyses indicate that the impact of the peak discharge is significant,whilst those of the Manningroughness,medium sediment size and shape of the inlet discharge hydrograph are marginal.

The first meteorological observations at a tropical high elevation site:Antisana,1846

Antisana is a stratovolcano with an associated glacier located in the Ecuadorian Andes. Dr Aguirre made meteorological readings every day, at every hour from sunrise to sunset, from December 1845 to December 1846, at Antisana using a meteorological station at 4060 mamsl （meters above mean sea level）. Unfortunately, only the monthly average data have been preserved. These meteorological data are here studied and compared with the closest modern stations for monthly values of temperature, rainfall, and pressure. According to these comparisons, the year 1846 was rainy and cold in comparison with the current climate. Moreover, these observations have been useful to help resolve a debate about a possible E1Nifio event in 1846 with the high precipitation in Antisana and Quito in 1846 discarding the occurrence of an E1 Nifio event. The probable occurrence of a La Nifia event is discussed. These data are the earliest known systematic instrumental meteorological observations taken at above 4000 mamsl.