EREBP/AP2-type proteins are members of a large DNA binding protein (DBP) family found in plants. Some members like APETALA2 and AtDREB/CBF can regulate flower development and response to environmental stresses, respec...EREBP/AP2-type proteins are members of a large DNA binding protein (DBP) family found in plants. Some members like APETALA2 and AtDREB/CBF can regulate flower development and response to environmental stresses, respectively. To characterize transcription factors involved in plant responses to salt stress, we constructed cDNA library from salt-treated halophyte (Atriplex hortensis) and isolated a novel gene encoding EREBP/AP2-type protein from this library. This cDNA contained an ORF of 723 bp and a long 3'-Untranslated-Region (UTR) of 655 bp. The deduced amino acid sequence showed one conserved DNA binding domain of EREBP/AP2, thus the corresponding gene was named AhDREB1 with a calculated molecular mass of 26.1 kD. AhDREB1 under the control of CaMV 35S promoter was then transformed into tobacco and nine independent transgenic lines were obtained and subjected to long term salt stress. The results suggested that overexpression of AhDREB1 improved the salt tolerance in transgenic tobacco through functioning as a regulatory molecule in response to salt stress. Analysis of Arabidopsis genome in database resulted in dozens of EREBP/AP2-type homologous proteins, of which seven members showed high similarity to AhDREB1. Secondary structure analysis predicted similar arrangement of a-helix in their DNA binding domains.展开更多
The acidity regimes of representative soils on the north slope of the Changbai Mountains were examined through determinations of PH and pCa of the soil paste as well as in-site determinations. For soils under broad-le...The acidity regimes of representative soils on the north slope of the Changbai Mountains were examined through determinations of PH and pCa of the soil paste as well as in-site determinations. For soils under broad-leaf forest or broad-leaf-Korean pine forest, the pH decreased from the litter to lower layers gradually until it did not change or decreased further slightly. For soils under coniferous forest or Ermans birch forest,there was a minimum in pH at a depth of 3～6 cm where the content of humus was high. The pCa increased gradually from the soil surface downward to a constant value. The lime potential (pH-0.5pCa) showed a similar trend as the PH in its distribution. For a given soil, the measured pH value of the thick paste, ranging from 4.5 to 5.5, was lower by about 0.5 units than the value determined by the conventional method with a water to soil ratio of 5:1. The PH determined in site was even lower. It was found that there was a fairly close relationship between soil acidity and the type of vegetation. The pH showed a trend of decreasing from soils under broad-leaf forest through broad-leaf-conifer mixed forest and coniferous forest to Ermans birch forest, and the pCa showed an opposite trend in variation.展开更多
ObjectiveThis study aimed to investigate the effects of burley and Chinese cabbage intercropping on disease occurrence and soil fertility in order to further determine the optimal burley and Chinese cabbage intercropp...ObjectiveThis study aimed to investigate the effects of burley and Chinese cabbage intercropping on disease occurrence and soil fertility in order to further determine the optimal burley and Chinese cabbage intercropping cultivation mode. MethodsEffects of burley and Chinese cabbage intercropping on disease occurrence, burley agronomic traits, yield and output value, and soil nutrients were investigated. ResultsThe results showed that for Chinese cabbage, viral diseases was severer, but the soft rot was lighter in the field with burley and Chinese cabbage intercropping compared to that with monocropping, and for burley, the viral disease was severer in the field with burley and Chinese cabbage intercropping compared to that with monocropping, with no significant difference; under the burley and Chinese cabbage intercropping mode, nitrogen input had no effect on burley agronomic traits and no significant effect on the yield and output value, and there was minor change in tobacco chemical components; burley and Chinese cabbage intercropping had no or little influence on soil pH, and and no effect on rapidly available potassium content in soil, but there is descending tend in organic matter, nitrogen and available phosphorus content. ConclusionThe study provides data support for the determination of the optimal burley tobacco and cabbage intercropping mode and the coordination of the development of these two industries.展开更多
文摘EREBP/AP2-type proteins are members of a large DNA binding protein (DBP) family found in plants. Some members like APETALA2 and AtDREB/CBF can regulate flower development and response to environmental stresses, respectively. To characterize transcription factors involved in plant responses to salt stress, we constructed cDNA library from salt-treated halophyte (Atriplex hortensis) and isolated a novel gene encoding EREBP/AP2-type protein from this library. This cDNA contained an ORF of 723 bp and a long 3'-Untranslated-Region (UTR) of 655 bp. The deduced amino acid sequence showed one conserved DNA binding domain of EREBP/AP2, thus the corresponding gene was named AhDREB1 with a calculated molecular mass of 26.1 kD. AhDREB1 under the control of CaMV 35S promoter was then transformed into tobacco and nine independent transgenic lines were obtained and subjected to long term salt stress. The results suggested that overexpression of AhDREB1 improved the salt tolerance in transgenic tobacco through functioning as a regulatory molecule in response to salt stress. Analysis of Arabidopsis genome in database resulted in dozens of EREBP/AP2-type homologous proteins, of which seven members showed high similarity to AhDREB1. Secondary structure analysis predicted similar arrangement of a-helix in their DNA binding domains.
文摘The acidity regimes of representative soils on the north slope of the Changbai Mountains were examined through determinations of PH and pCa of the soil paste as well as in-site determinations. For soils under broad-leaf forest or broad-leaf-Korean pine forest, the pH decreased from the litter to lower layers gradually until it did not change or decreased further slightly. For soils under coniferous forest or Ermans birch forest,there was a minimum in pH at a depth of 3～6 cm where the content of humus was high. The pCa increased gradually from the soil surface downward to a constant value. The lime potential (pH-0.5pCa) showed a similar trend as the PH in its distribution. For a given soil, the measured pH value of the thick paste, ranging from 4.5 to 5.5, was lower by about 0.5 units than the value determined by the conventional method with a water to soil ratio of 5:1. The PH determined in site was even lower. It was found that there was a fairly close relationship between soil acidity and the type of vegetation. The pH showed a trend of decreasing from soils under broad-leaf forest through broad-leaf-conifer mixed forest and coniferous forest to Ermans birch forest, and the pCa showed an opposite trend in variation.
基金Supported by the Burley Brand Development Fund of Yichang City(201103)~~
文摘ObjectiveThis study aimed to investigate the effects of burley and Chinese cabbage intercropping on disease occurrence and soil fertility in order to further determine the optimal burley and Chinese cabbage intercropping cultivation mode. MethodsEffects of burley and Chinese cabbage intercropping on disease occurrence, burley agronomic traits, yield and output value, and soil nutrients were investigated. ResultsThe results showed that for Chinese cabbage, viral diseases was severer, but the soft rot was lighter in the field with burley and Chinese cabbage intercropping compared to that with monocropping, and for burley, the viral disease was severer in the field with burley and Chinese cabbage intercropping compared to that with monocropping, with no significant difference; under the burley and Chinese cabbage intercropping mode, nitrogen input had no effect on burley agronomic traits and no significant effect on the yield and output value, and there was minor change in tobacco chemical components; burley and Chinese cabbage intercropping had no or little influence on soil pH, and and no effect on rapidly available potassium content in soil, but there is descending tend in organic matter, nitrogen and available phosphorus content. ConclusionThe study provides data support for the determination of the optimal burley tobacco and cabbage intercropping mode and the coordination of the development of these two industries.
