[Objective] The research aimed to study the transferability of Camellia sinensis EST-SSRs in Theaceae plant.[Method] Seven pairs of EST-SSRs primers which derived from Camellia sinensis EST sequence were used to ampli...[Objective] The research aimed to study the transferability of Camellia sinensis EST-SSRs in Theaceae plant.[Method] Seven pairs of EST-SSRs primers which derived from Camellia sinensis EST sequence were used to amplify the nineteen materials of Theaceae plant.[Result] Five pairs in the seven pairs of EST-SSRs primers could effectively amplify the nineteen tested varieties,and the transferability rate was 71.43%.The amplification rate of Camellia retiacalate FengShanCha,Camellia japouica CaiXia and Camellia retiacalate JuBan was the highest.The amplification rate of Camellia synaptica Sealy and Adinandra sagonica var.wallichiana（oc）Ming was the lowest.Moreover,four pairs in the five pairs of primers which could effectively amplify showed the rich polymorphism whose difference was obvious in the tested materials.[Conclusion] SSR primers which were developed from Camellia sinensis genome had the higher transferability in the different genus and species of Theaceae plant,could be used in the comparative genome research and analysis mark research of Theaceae plant.展开更多
The Nilgiri Mountains of south India isconsidered unique by anthropologists, geologists,climatologists, botanists as well as tourists. It hasremained a subject of constant study and researchover the last two centuries...The Nilgiri Mountains of south India isconsidered unique by anthropologists, geologists,climatologists, botanists as well as tourists. It hasremained a subject of constant study and researchover the last two centuries. Man-nature balance had continued undisturbed in the Nilgiris for thousandsof years until the early 19th century when it became a British colony attracting, in due course, variousdevelopmental activities. Subsequently, the Nilgirisand its popular hill stations emerged as favouriteplaces for the British population in India for rest and recuperation, game and for raising commercial plantations. In the process, the traditional indigenous crops were replaced by 揈nglish?vegetables and the natural forests gave way to commercial plantations of coffee, tea and other exotic species of trees. After Independence in 1947, the government of India accelerated the developmental process on thesame lines as during the colonial period leading to arapid growth of urbanisation and commercialplantations. Increasing pressure on land foragriculture and monoculture plantations displaced an alarmingly high proportion of natural forests andgrasslands leading to an extensive loss of biodiversity and turning the Nilgiris into a biodiversity 揾otspot? as identified by World Wildlife Fund, India (1995).Mindless development since the 1970s further tilted the scale precariously, pushing the hills to the brink of an ecological disaster. Nilgiris entered an anxious era of landslides, which have become more frequent and disastrous in recent decades. The 揜eport on the study of Landslides of November 1993 in Nilgiris District?observed that 搊ccurrence of land-slides in Nilgiris, particularly at the onset and during the north-east monsoons, is a ubiquitous, recurring, annual phenomenon? The colonists simultaneously developed the Nilgiris as a tourist resort for the English population. When independence came, the English were replaced by the Indian princely classes, politicians, capitalists and bureaucrats. After the 1970s, tourism became a mass industry for various reasons. Tourist arrivals increased exponentially to cross a million a year since 2000. However, without a proper plan to promote it on desired lines, the lop-sided and haphazard growth of tourism brought more harm than good to the hills. Alongside, unrelenting commercialization and immigration explosion with no corresponding improvements in infrastructures and amenities have begun to strain the carrying capacity of the hills, leading to water famine, pollution, urban congestion and marginalisation of the indigenous people. The Nilgiris is at the cross roads in the 21st century. Its development appears to have reached its limits with the predominant plantation economy collapsing and its tourism industry stagnating. Any further shifts in land use or cropping pattern appear economically unsound and ecologically catastrophic. Promotion of tourism again may prove counter productive unless there is a radical change in the focus and objectives of the industry in consonance with the overall priorities of the district. The Nilgiris is desperately looking for the best international practices to balance the needs of development and conservation.展开更多
To explore the volatile profiles and the contents of ten bioactive components(polyphenols and caffeine) of sun-dried Pu-erh tea leaves from ancient tea plants on Bulang Mountain,17 samples of three tea varieties were ...To explore the volatile profiles and the contents of ten bioactive components(polyphenols and caffeine) of sun-dried Pu-erh tea leaves from ancient tea plants on Bulang Mountain,17 samples of three tea varieties were analyzed by headspace-solid phase microextraction-gas chromatography-mass spectrometry(HS-SPME-GC-MS) and high-performance liquid chromatography(HPLC).A total of 75 volatile components were tentatively identified.Laomaner(LME),Laobanzhang(LBZ),and other teas on Bulang Mountain(BL) contained 70,53,and 71 volatile compounds,respectively.Among the volatile compounds,alcohols(30.2%-45.8%),hydrocarbons(13.7%-17.5%),and ketones(12.4%-23.4%) were qualitatively the most dominant volatile compounds in the different tea varieties.The average content of polyphenol was highest in LME(102.1 mg/g),followed by BL(98.7 mg/g) and LBZ(88.0 mg/g),while caffeine showed the opposite trend,27.3 mg/g in LME,33.5 mg/g in BL,and 38.1 mg/g in LBZ.Principal component analysis applied to both the volatile compounds and ten bioactive components showed a poor separation of samples according to varieties,while partial least squares-discriminant analysis(PLS-DA) showed satisfactory discrimination.Thirty-four volatile components and five bioactive compounds were selected as major discriminators(variable importance in projection(VIP)>1) among the tea varieties.These results suggest that chromatographic data combined with multivariate analysis could provide a useful technique to characterize and distinguish the sun-dried Pu-erh tea leaves from ancient tea varieties on Bulang Mountain.展开更多
基金Supported by"Provincial and Ministerial Key Discipline,Provincial Key Laboratory of University and School Laboratory Sharing Platform" ItemSouthwest Forestry University Key Fund Item(110909)~~
文摘[Objective] The research aimed to study the transferability of Camellia sinensis EST-SSRs in Theaceae plant.[Method] Seven pairs of EST-SSRs primers which derived from Camellia sinensis EST sequence were used to amplify the nineteen materials of Theaceae plant.[Result] Five pairs in the seven pairs of EST-SSRs primers could effectively amplify the nineteen tested varieties,and the transferability rate was 71.43%.The amplification rate of Camellia retiacalate FengShanCha,Camellia japouica CaiXia and Camellia retiacalate JuBan was the highest.The amplification rate of Camellia synaptica Sealy and Adinandra sagonica var.wallichiana（oc）Ming was the lowest.Moreover,four pairs in the five pairs of primers which could effectively amplify showed the rich polymorphism whose difference was obvious in the tested materials.[Conclusion] SSR primers which were developed from Camellia sinensis genome had the higher transferability in the different genus and species of Theaceae plant,could be used in the comparative genome research and analysis mark research of Theaceae plant.
文摘The Nilgiri Mountains of south India isconsidered unique by anthropologists, geologists,climatologists, botanists as well as tourists. It hasremained a subject of constant study and researchover the last two centuries. Man-nature balance had continued undisturbed in the Nilgiris for thousandsof years until the early 19th century when it became a British colony attracting, in due course, variousdevelopmental activities. Subsequently, the Nilgirisand its popular hill stations emerged as favouriteplaces for the British population in India for rest and recuperation, game and for raising commercial plantations. In the process, the traditional indigenous crops were replaced by 揈nglish?vegetables and the natural forests gave way to commercial plantations of coffee, tea and other exotic species of trees. After Independence in 1947, the government of India accelerated the developmental process on thesame lines as during the colonial period leading to arapid growth of urbanisation and commercialplantations. Increasing pressure on land foragriculture and monoculture plantations displaced an alarmingly high proportion of natural forests andgrasslands leading to an extensive loss of biodiversity and turning the Nilgiris into a biodiversity 揾otspot? as identified by World Wildlife Fund, India (1995).Mindless development since the 1970s further tilted the scale precariously, pushing the hills to the brink of an ecological disaster. Nilgiris entered an anxious era of landslides, which have become more frequent and disastrous in recent decades. The 揜eport on the study of Landslides of November 1993 in Nilgiris District?observed that 搊ccurrence of land-slides in Nilgiris, particularly at the onset and during the north-east monsoons, is a ubiquitous, recurring, annual phenomenon? The colonists simultaneously developed the Nilgiris as a tourist resort for the English population. When independence came, the English were replaced by the Indian princely classes, politicians, capitalists and bureaucrats. After the 1970s, tourism became a mass industry for various reasons. Tourist arrivals increased exponentially to cross a million a year since 2000. However, without a proper plan to promote it on desired lines, the lop-sided and haphazard growth of tourism brought more harm than good to the hills. Alongside, unrelenting commercialization and immigration explosion with no corresponding improvements in infrastructures and amenities have begun to strain the carrying capacity of the hills, leading to water famine, pollution, urban congestion and marginalisation of the indigenous people. The Nilgiris is at the cross roads in the 21st century. Its development appears to have reached its limits with the predominant plantation economy collapsing and its tourism industry stagnating. Any further shifts in land use or cropping pattern appear economically unsound and ecologically catastrophic. Promotion of tourism again may prove counter productive unless there is a radical change in the focus and objectives of the industry in consonance with the overall priorities of the district. The Nilgiris is desperately looking for the best international practices to balance the needs of development and conservation.
文摘To explore the volatile profiles and the contents of ten bioactive components(polyphenols and caffeine) of sun-dried Pu-erh tea leaves from ancient tea plants on Bulang Mountain,17 samples of three tea varieties were analyzed by headspace-solid phase microextraction-gas chromatography-mass spectrometry(HS-SPME-GC-MS) and high-performance liquid chromatography(HPLC).A total of 75 volatile components were tentatively identified.Laomaner(LME),Laobanzhang(LBZ),and other teas on Bulang Mountain(BL) contained 70,53,and 71 volatile compounds,respectively.Among the volatile compounds,alcohols(30.2%-45.8%),hydrocarbons(13.7%-17.5%),and ketones(12.4%-23.4%) were qualitatively the most dominant volatile compounds in the different tea varieties.The average content of polyphenol was highest in LME(102.1 mg/g),followed by BL(98.7 mg/g) and LBZ(88.0 mg/g),while caffeine showed the opposite trend,27.3 mg/g in LME,33.5 mg/g in BL,and 38.1 mg/g in LBZ.Principal component analysis applied to both the volatile compounds and ten bioactive components showed a poor separation of samples according to varieties,while partial least squares-discriminant analysis(PLS-DA) showed satisfactory discrimination.Thirty-four volatile components and five bioactive compounds were selected as major discriminators(variable importance in projection(VIP)>1) among the tea varieties.These results suggest that chromatographic data combined with multivariate analysis could provide a useful technique to characterize and distinguish the sun-dried Pu-erh tea leaves from ancient tea varieties on Bulang Mountain.
