Lewis X(Le x) is stage specifically expressed on the surface of mammalian embryos. Histochemical evidences have showed that Le x is expressed in endometrium, and may be controlled by ovarian hormones. It has been repo...Lewis X(Le x) is stage specifically expressed on the surface of mammalian embryos. Histochemical evidences have showed that Le x is expressed in endometrium, and may be controlled by ovarian hormones. It has been reported that FUT9 gene is the real specific gene encoding α1, 3 fucosyltransferase for the synthesis of terminal fucose of Le x. In this article, the transcription of FUT9 and expression of Le x oligosaccharide antigen in mouse endometrium were investigated. The mice(22～25 g) were selected and treated as follows:(1) Estrous: females were checked by vaginal smears to determine their stage of estrus;(2) Early pregnancy: females were mated with males(1∶1), and the day of vaginal plug appearance was considered as day 1 of pregnancy;(3) Hormone replacement: after ovariectomy, mice were given subcutaneously estradiol benzoate and progesterone daily for 4 days. The results showed that the transcription level of FUT9 gene was:(1) periodically changed during estrous cycle: highest at estrus stage, and lowest at metestrus stage;(2) decreased obviously during early pregnancy, then maintained at a lower level from day 3 to day 5;(3) increased in the estradiol treatment group, and decreased obviously in the progesterone treatment group, and was at a middle level in the group treated with both estradiol and progesterone;(4) level of Le x carrying proteins on the surface of endometrium did it coincide fully with that of FUT9 gene transcription, which indicated that other factors may be also involved in the regulation of Le x expression in addition to the hormones. In summary, the expression of FUT9 gene in mouse endometrium is regulated by ovarian hormones, being up regulated by estrogen and down regulated by progesterone.展开更多
目的:探讨输卵管上皮内N-乙酰葡萄糖胺-6-转磺酶(CHST2)和岩藻糖基转移酶7(Fuc T-VII)表达与输卵管妊娠发生的关系。方法:采用免疫组织化学和实时荧光定量PCR(RTPCR)的方法,检测输卵管妊娠患者的输卵管胚囊着床部位组织(EP-2组,n=23),...目的:探讨输卵管上皮内N-乙酰葡萄糖胺-6-转磺酶(CHST2)和岩藻糖基转移酶7(Fuc T-VII)表达与输卵管妊娠发生的关系。方法:采用免疫组织化学和实时荧光定量PCR(RTPCR)的方法,检测输卵管妊娠患者的输卵管胚囊着床部位组织(EP-2组,n=23),输卵管胚囊着床旁部位组织(EP-1组,n=23),正常输卵管组织(CT组,n=16)的CHST2和Fuc T-VII的表达情况。结果:免疫组织化学法检测到输卵管妊娠胚囊着床部位CHST2和Fuc T-VII表达明显上调,与正常组相比差异有显著统计学意义(P<0.05)。RT-PCR方法检测CHST2 m RNA和Fuc T-VII m RNA在输卵管妊娠胚囊着床部表达水平高于正常组,差异有显著统计学意义(P<0.05)。结论:L-选择素与其配体相结合的机制可能参与了输卵管妊娠的发生。展开更多
文摘Lewis X(Le x) is stage specifically expressed on the surface of mammalian embryos. Histochemical evidences have showed that Le x is expressed in endometrium, and may be controlled by ovarian hormones. It has been reported that FUT9 gene is the real specific gene encoding α1, 3 fucosyltransferase for the synthesis of terminal fucose of Le x. In this article, the transcription of FUT9 and expression of Le x oligosaccharide antigen in mouse endometrium were investigated. The mice(22～25 g) were selected and treated as follows:(1) Estrous: females were checked by vaginal smears to determine their stage of estrus;(2) Early pregnancy: females were mated with males(1∶1), and the day of vaginal plug appearance was considered as day 1 of pregnancy;(3) Hormone replacement: after ovariectomy, mice were given subcutaneously estradiol benzoate and progesterone daily for 4 days. The results showed that the transcription level of FUT9 gene was:(1) periodically changed during estrous cycle: highest at estrus stage, and lowest at metestrus stage;(2) decreased obviously during early pregnancy, then maintained at a lower level from day 3 to day 5;(3) increased in the estradiol treatment group, and decreased obviously in the progesterone treatment group, and was at a middle level in the group treated with both estradiol and progesterone;(4) level of Le x carrying proteins on the surface of endometrium did it coincide fully with that of FUT9 gene transcription, which indicated that other factors may be also involved in the regulation of Le x expression in addition to the hormones. In summary, the expression of FUT9 gene in mouse endometrium is regulated by ovarian hormones, being up regulated by estrogen and down regulated by progesterone.
文摘目的:探讨输卵管上皮内N-乙酰葡萄糖胺-6-转磺酶(CHST2)和岩藻糖基转移酶7(Fuc T-VII)表达与输卵管妊娠发生的关系。方法:采用免疫组织化学和实时荧光定量PCR(RTPCR)的方法,检测输卵管妊娠患者的输卵管胚囊着床部位组织(EP-2组,n=23),输卵管胚囊着床旁部位组织(EP-1组,n=23),正常输卵管组织(CT组,n=16)的CHST2和Fuc T-VII的表达情况。结果:免疫组织化学法检测到输卵管妊娠胚囊着床部位CHST2和Fuc T-VII表达明显上调,与正常组相比差异有显著统计学意义(P<0.05)。RT-PCR方法检测CHST2 m RNA和Fuc T-VII m RNA在输卵管妊娠胚囊着床部表达水平高于正常组,差异有显著统计学意义(P<0.05)。结论:L-选择素与其配体相结合的机制可能参与了输卵管妊娠的发生。
