AIM: To evaluate the killing effect of double suicide gene mediated by adenovirus and regulated under kinase domain insert containing receptor (KDR) promoter on human umbilical vein endothelial cells. METHODS: By PCR ...AIM: To evaluate the killing effect of double suicide gene mediated by adenovirus and regulated under kinase domain insert containing receptor (KDR) promoter on human umbilical vein endothelial cells. METHODS: By PCR technology, human KDR promoter gene, Escherichia coli(E. coli) cytosine deaminase (CD) gene and the herpes simple virus-thymidine kinase (TK) gene were cloned. Plasmid pKDR-CDglyTK was constructed with them. Then, a recombinant adenoviral plasmid pAdKDRCDglyTK was constructed in a 'two-step transformation protocol'. The newly constructed plasmids were transfected to 293 packaging cells to grow adenoviruses, which were further propagated and purified. Human umbilical vein endothelial cells (HUVEC) were infected with a different multiplicity of infection (MOI) of resultant recombinant adenovirus, the infection rate was measured with the aid of (GFP) expression. Infected cells were cultured in culture media containing different concentrations of (GCV) and/or 5-(FC), and the killing effects were measured.RESULTS: Recombinant adenoviruses AdKDR-CDglyTK were successfully constructed, and they infected HUVEC cells efficiently. Our data indicated that the infection rate was relevant to MOI of recombinant adenoviruses. HUVEC cells infected with AdKDR-CDglyTK were highly sensitive to the prodrugs, their survival rate correlated to both the concentration of the prodrugs and the MOI of recombinant adenoviruses. Our data also indicated that the two prodrugs used in combination were much more effective on killing transgeneic cells than GCV or 5-FC used alone. CONCLUSION: Prodrug/KDR-CDglyTK system is effective on killing HUVEC cells, its killing effect correlates to the concentration of prodrugs and recombinant adenovirus' MOI. Combined use of the two prodrugs confers better killing effects on transgeneic cells.展开更多
JPEG-Compatibility steganalysis detects the presence of secret message embedded in the JPEG decompressed images and estimates the embedding rate. We propose a JPEG-Compatibility steganalysis algorithm that estimates t...JPEG-Compatibility steganalysis detects the presence of secret message embedded in the JPEG decompressed images and estimates the embedding rate. We propose a JPEG-Compatibility steganalysis algorithm that estimates the embedding rate based on the difference between the stego image and its recompression based predicted co-vet image. In particular, compression artifacts and embedding changes are distinguished based on the amplitude of pixel value changes. This is done independent of the embedding positions, thus is effective for both content non-adaptive and content adaptive steganography. In addition, we also improve the recompression prediction scheme to more accurately estimate the JPEG quantization table. Experimental results show that the proposed algorithm is significantly more effective in detecting spatial ±1 steganography across a wide range of quality factors and embedding rates, when compared to the previous works.展开更多
The wide variety of smart embedded computing devices and their increasing number of applications in our daily life have created new op- portunities to acquire knowledge from the physical world anytime and anywhere, wh...The wide variety of smart embedded computing devices and their increasing number of applications in our daily life have created new op- portunities to acquire knowledge from the physical world anytime and anywhere, which is envisioned as the＂Internet of Things＂ （IoT）. Since a huge number of heterogeneous resources are brought in- to IoT, one of the main challenges is how to effi- ciently manage the increasing complexity of IoT in a scalable, flexNle, and autonomic way. Further- more, the emerging IoT applications will require collaborations among loosely coupled devices, which may reside in various locations of the Inter- net. In this paper, we propose a new IoT network management architecture based on cognitive net- work management technology and Service-Orien- ted Architecture to provide effective and efficient network management of loT.展开更多
随着时代的不断发展以及进步,我们可以发现其物联网技术在我国取得了较快的进步。其中,对于物联网而言,它主要是通过互联网的方式向用户的 PC 端以及手机收集数据,进而对网关设备开展设计。因此在本篇文章中,我们主要是简单的探讨 STM32...随着时代的不断发展以及进步,我们可以发现其物联网技术在我国取得了较快的进步。其中,对于物联网而言,它主要是通过互联网的方式向用户的 PC 端以及手机收集数据,进而对网关设备开展设计。因此在本篇文章中,我们主要是简单的探讨 STM32 的物联网嵌入式网关设计分析。展开更多
From the view of practical application, this paper designs an infrared information collection nodes, the coordinator node, wireless transmission system using CC2430 microcontroller, and in detail design the infrared s...From the view of practical application, this paper designs an infrared information collection nodes, the coordinator node, wireless transmission system using CC2430 microcontroller, and in detail design the infrared sensor （temperature, heart rate, ECG / pulse） circuit diagram. This paper set up a wireless network （ZigBee protocol）, sensor signal acquisition, wireless transmitting and receiving, serial communication software design of coordinator node and PC machine. Finally, we on-line debug each module of the system hardware and software. Experiments show that the network node data transmission accurate, reliable work, has basically met the design requirements.展开更多
Using physical model and numerical simulation techniques, some technical problems were studied systemati- cally, including layout of power station, measures of sediment and floating debris discharging, types of intake...Using physical model and numerical simulation techniques, some technical problems were studied systemati- cally, including layout of power station, measures of sediment and floating debris discharging, types of intake, embed- ded types of spiral ease, layout of underground powerhouse tunnel group and block reinforcement. It was optimal in technique and economy with the arrangement of powerhouse at the dam-toe of both banks ＋ underground powerhouse in the right bank, as well as the intake with a single and small orifice. The sediment and debris problems could be solved with disperse sediment ejection and floating debris discharging holes. With the adoption of techniques for spiral cases such as heat and pressure preservation, cushion layer and combined embedding, the stable operation of generating units can be guaranteed. The arrangement of tailrace tunnel with sloping ceiling was better than that of tailrace surge tank. The technical requirements related to the embedding type of spiral case were proposed. The reinforcement of huge unfavorable blocks was discussed and the new idea for block reinforcement using anti-sliding piles and normal compressive stress of structural plane was put forward.展开更多
基金Supported by the Natural Science Foundation of Guangdong Province, No. 013072and the 863 Program, No. 2001AA217171
文摘AIM: To evaluate the killing effect of double suicide gene mediated by adenovirus and regulated under kinase domain insert containing receptor (KDR) promoter on human umbilical vein endothelial cells. METHODS: By PCR technology, human KDR promoter gene, Escherichia coli(E. coli) cytosine deaminase (CD) gene and the herpes simple virus-thymidine kinase (TK) gene were cloned. Plasmid pKDR-CDglyTK was constructed with them. Then, a recombinant adenoviral plasmid pAdKDRCDglyTK was constructed in a 'two-step transformation protocol'. The newly constructed plasmids were transfected to 293 packaging cells to grow adenoviruses, which were further propagated and purified. Human umbilical vein endothelial cells (HUVEC) were infected with a different multiplicity of infection (MOI) of resultant recombinant adenovirus, the infection rate was measured with the aid of (GFP) expression. Infected cells were cultured in culture media containing different concentrations of (GCV) and/or 5-(FC), and the killing effects were measured.RESULTS: Recombinant adenoviruses AdKDR-CDglyTK were successfully constructed, and they infected HUVEC cells efficiently. Our data indicated that the infection rate was relevant to MOI of recombinant adenoviruses. HUVEC cells infected with AdKDR-CDglyTK were highly sensitive to the prodrugs, their survival rate correlated to both the concentration of the prodrugs and the MOI of recombinant adenoviruses. Our data also indicated that the two prodrugs used in combination were much more effective on killing transgeneic cells than GCV or 5-FC used alone. CONCLUSION: Prodrug/KDR-CDglyTK system is effective on killing HUVEC cells, its killing effect correlates to the concentration of prodrugs and recombinant adenovirus' MOI. Combined use of the two prodrugs confers better killing effects on transgeneic cells.
基金This work was supported in part by National Natural Science Foundation of China，National 973 Basic Research Program of China，the Fundamental Research Funds for the Central Universities,HUST
文摘JPEG-Compatibility steganalysis detects the presence of secret message embedded in the JPEG decompressed images and estimates the embedding rate. We propose a JPEG-Compatibility steganalysis algorithm that estimates the embedding rate based on the difference between the stego image and its recompression based predicted co-vet image. In particular, compression artifacts and embedding changes are distinguished based on the amplitude of pixel value changes. This is done independent of the embedding positions, thus is effective for both content non-adaptive and content adaptive steganography. In addition, we also improve the recompression prediction scheme to more accurately estimate the JPEG quantization table. Experimental results show that the proposed algorithm is significantly more effective in detecting spatial ±1 steganography across a wide range of quality factors and embedding rates, when compared to the previous works.
基金supported by the National Sci.&Tech. Major Project of China(No.2010ZX03004-002)the National Natural Science Foundation of China(No.60972083)
文摘The wide variety of smart embedded computing devices and their increasing number of applications in our daily life have created new op- portunities to acquire knowledge from the physical world anytime and anywhere, which is envisioned as the＂Internet of Things＂ （IoT）. Since a huge number of heterogeneous resources are brought in- to IoT, one of the main challenges is how to effi- ciently manage the increasing complexity of IoT in a scalable, flexNle, and autonomic way. Further- more, the emerging IoT applications will require collaborations among loosely coupled devices, which may reside in various locations of the Inter- net. In this paper, we propose a new IoT network management architecture based on cognitive net- work management technology and Service-Orien- ted Architecture to provide effective and efficient network management of loT.
文摘From the view of practical application, this paper designs an infrared information collection nodes, the coordinator node, wireless transmission system using CC2430 microcontroller, and in detail design the infrared sensor （temperature, heart rate, ECG / pulse） circuit diagram. This paper set up a wireless network （ZigBee protocol）, sensor signal acquisition, wireless transmitting and receiving, serial communication software design of coordinator node and PC machine. Finally, we on-line debug each module of the system hardware and software. Experiments show that the network node data transmission accurate, reliable work, has basically met the design requirements.
文摘Using physical model and numerical simulation techniques, some technical problems were studied systemati- cally, including layout of power station, measures of sediment and floating debris discharging, types of intake, embed- ded types of spiral ease, layout of underground powerhouse tunnel group and block reinforcement. It was optimal in technique and economy with the arrangement of powerhouse at the dam-toe of both banks ＋ underground powerhouse in the right bank, as well as the intake with a single and small orifice. The sediment and debris problems could be solved with disperse sediment ejection and floating debris discharging holes. With the adoption of techniques for spiral cases such as heat and pressure preservation, cushion layer and combined embedding, the stable operation of generating units can be guaranteed. The arrangement of tailrace tunnel with sloping ceiling was better than that of tailrace surge tank. The technical requirements related to the embedding type of spiral case were proposed. The reinforcement of huge unfavorable blocks was discussed and the new idea for block reinforcement using anti-sliding piles and normal compressive stress of structural plane was put forward.
