Objectives.: To evaluate the efficacy of self-collected vaginal samples for high-risk HPV detection by the HPV oligonucleotide microarray method(HPVDNAChipTM). Me-thods.: One hundred and eighteen patients with abnorma...Objectives.: To evaluate the efficacy of self-collected vaginal samples for high-risk HPV detection by the HPV oligonucleotide microarray method(HPVDNAChipTM). Me-thods.: One hundred and eighteen patients with abnormal Pap smears were included. Self-collected vaginal and clinician-collected cervical samples for HPV testing were obtained. The result of the HPV DNA test was compared with the histopathological diagnosis or colposcopic finding. Results.: Of the 118 patients, 42(35.6%) had ≥cervical intraepithelial neoplasia(CIN) III lesions. Using the HPVDNAChipTM, high-risk types of HPV were detected in 38 of these 42 patients(90.5%) with the self-collected vaginal samples and in 37 of 42(88.1%) with the clini-cian-collected cervical samples. The agreement of HPVDNAchip TM results between self-and clinician-collected samples was very good(κ=0.81) with a 93.2%concordance rate. Multiple HPV infections were found in 17 of 88(19.3%)-HPV-positive clinician-collected cervical samples. The rate of multiple HPV infection tended to decrease as the degree of pathologic classification increased. Conclusion.: Using the HPVDNAchipTM to assay for HPV infection, results from selfcollected vaginal samples were compatible with those from clinician-collected cervical samples.展开更多
Cyclic voltammetry(CV)and ultravioletvisible spectroscopy(UV) were used to study cerium amino acid complexes.The amino acids are Phenylananine,Histidine,Serine,Threonine and Glutamicacid.These amino acids can coor...Cyclic voltammetry(CV)and ultravioletvisible spectroscopy(UV) were used to study cerium amino acid complexes.The amino acids are Phenylananine,Histidine,Serine,Threonine and Glutamicacid.These amino acids can coordinate with cerium ion(Ⅳ) to form complexes at near neutral condition(pH=7).At the same time,the denaturing ployacrylamidegel electrophoresissilver dye method was used to analyze the hydrolysis of 26mers oligodeoxyribonucleotide by cerium amino acid complexes around the neutral condition(pH=7).The results show that these cerium amino acid complexes can cleavage DNA at different degree.We also analyze the possible mechanism of the different hydrolysis effects.展开更多
文摘Objectives.: To evaluate the efficacy of self-collected vaginal samples for high-risk HPV detection by the HPV oligonucleotide microarray method(HPVDNAChipTM). Me-thods.: One hundred and eighteen patients with abnormal Pap smears were included. Self-collected vaginal and clinician-collected cervical samples for HPV testing were obtained. The result of the HPV DNA test was compared with the histopathological diagnosis or colposcopic finding. Results.: Of the 118 patients, 42(35.6%) had ≥cervical intraepithelial neoplasia(CIN) III lesions. Using the HPVDNAChipTM, high-risk types of HPV were detected in 38 of these 42 patients(90.5%) with the self-collected vaginal samples and in 37 of 42(88.1%) with the clini-cian-collected cervical samples. The agreement of HPVDNAchip TM results between self-and clinician-collected samples was very good(κ=0.81) with a 93.2%concordance rate. Multiple HPV infections were found in 17 of 88(19.3%)-HPV-positive clinician-collected cervical samples. The rate of multiple HPV infection tended to decrease as the degree of pathologic classification increased. Conclusion.: Using the HPVDNAchipTM to assay for HPV infection, results from selfcollected vaginal samples were compatible with those from clinician-collected cervical samples.
文摘Cyclic voltammetry(CV)and ultravioletvisible spectroscopy(UV) were used to study cerium amino acid complexes.The amino acids are Phenylananine,Histidine,Serine,Threonine and Glutamicacid.These amino acids can coordinate with cerium ion(Ⅳ) to form complexes at near neutral condition(pH=7).At the same time,the denaturing ployacrylamidegel electrophoresissilver dye method was used to analyze the hydrolysis of 26mers oligodeoxyribonucleotide by cerium amino acid complexes around the neutral condition(pH=7).The results show that these cerium amino acid complexes can cleavage DNA at different degree.We also analyze the possible mechanism of the different hydrolysis effects.
