目的采用半巢式多重PCR法对A组轮状病毒(group A rotavirus,RVA)VP7基因进行分型。方法根据GenBank中RVA VP7基因5′端保守区设计合成多组基因分型引物,提取11个RVA代表流行毒株病毒的RNA,采用半巢式多重PCR方法进行RVA的VP7基因分型,使...目的采用半巢式多重PCR法对A组轮状病毒(group A rotavirus,RVA)VP7基因进行分型。方法根据GenBank中RVA VP7基因5′端保守区设计合成多组基因分型引物,提取11个RVA代表流行毒株病毒的RNA,采用半巢式多重PCR方法进行RVA的VP7基因分型,使用BLAST在线软件与GenBank上相应VP7核苷酸序列比对。结果经琼脂糖凝胶电泳鉴定,11株RVA的多重PCR产物大小均与预期一致,能正确鉴别具有代表性的流行毒株VP7基因型[G1、G2、KN105(Wa-like G3)、To16-01(equine-like,DS-1-likeG3)、G4、G8、G9及G12];经BLAST在线软件比对,RVA病毒株VP7基因序列与设计的上游引物匹配,并与下游引物互补,引物特异性良好。结论利用设计的引物成功建立的半巢式多重PCR方法能正确识别RVA的VP7基因型。展开更多
In order to develop an assay to distinguish the infection of Equine infectious anemia virus(EIAV)American strains from Donkey-leukocyte attenuated virus(DLV)strain,eight primers were designed based on the comparison o...In order to develop an assay to distinguish the infection of Equine infectious anemia virus(EIAV)American strains from Donkey-leukocyte attenuated virus(DLV)strain,eight primers were designed based on the comparison of complete sequences of four EIAV American strains and DLV.Corresponding viral genome fragments were amplified and analyzed from donkey leucocytes by PCR using these eight primers.Four primers from gag gene were selected to detect the differences between EIAV American strains and DLV.Six horses were inoculated with DLV and two with EIAV American strains,the Wyoming strain and the Argentina strain,respectively.Two fragments,675bp and 400bp,were amplified from DLV-infected cells,while only one segment of 675bp was obtained from cells inoculated with American strains.Our results indicate that this nest multiplex PCR can be used to distinguish EIAV American strains from the donkey leucocyte-attenuated vaccine strain of EIAV.展开更多
文摘目的采用半巢式多重PCR法对A组轮状病毒(group A rotavirus,RVA)VP7基因进行分型。方法根据GenBank中RVA VP7基因5′端保守区设计合成多组基因分型引物,提取11个RVA代表流行毒株病毒的RNA,采用半巢式多重PCR方法进行RVA的VP7基因分型,使用BLAST在线软件与GenBank上相应VP7核苷酸序列比对。结果经琼脂糖凝胶电泳鉴定,11株RVA的多重PCR产物大小均与预期一致,能正确鉴别具有代表性的流行毒株VP7基因型[G1、G2、KN105(Wa-like G3)、To16-01(equine-like,DS-1-likeG3)、G4、G8、G9及G12];经BLAST在线软件比对,RVA病毒株VP7基因序列与设计的上游引物匹配,并与下游引物互补,引物特异性良好。结论利用设计的引物成功建立的半巢式多重PCR方法能正确识别RVA的VP7基因型。
文摘In order to develop an assay to distinguish the infection of Equine infectious anemia virus(EIAV)American strains from Donkey-leukocyte attenuated virus(DLV)strain,eight primers were designed based on the comparison of complete sequences of four EIAV American strains and DLV.Corresponding viral genome fragments were amplified and analyzed from donkey leucocytes by PCR using these eight primers.Four primers from gag gene were selected to detect the differences between EIAV American strains and DLV.Six horses were inoculated with DLV and two with EIAV American strains,the Wyoming strain and the Argentina strain,respectively.Two fragments,675bp and 400bp,were amplified from DLV-infected cells,while only one segment of 675bp was obtained from cells inoculated with American strains.Our results indicate that this nest multiplex PCR can be used to distinguish EIAV American strains from the donkey leucocyte-attenuated vaccine strain of EIAV.