[Objective] This study aimed to analyze the quantitative trait loci （QTLs） of grain storage durability for maize under controlled deterioration conditions by using SSR markers. [Method] The recombinant inbred line p...[Objective] This study aimed to analyze the quantitative trait loci （QTLs） of grain storage durability for maize under controlled deterioration conditions by using SSR markers. [Method] The recombinant inbred line population F 2 derived from the cross of shen 137/02-50 was used to do the simple sequence repeats （SSRs） and QTL analysis. [Result] The 2 parents showed good polymorphisms. Three loci were detected on chromosome 1, chromosome 6 and chromosome 9, accounting for 41.2% of total phenotypic. [Conclusion] This study provided theoretical basis for using molecular marker to assist the breeding of storage durable maize varieties.展开更多
Vertical polyamide gel electrophoresis was used to investigate isozyme polymorphisms among different isolates （including wild and cultivated） of Porphyra katadai, Porphyra oligospermatangia, Porphyra yezoensis, Porp...Vertical polyamide gel electrophoresis was used to investigate isozyme polymorphisms among different isolates （including wild and cultivated） of Porphyra katadai, Porphyra oligospermatangia, Porphyra yezoensis, Porphyra haitanensis, and a hybridize species （Porphyra yezoensis x Porphyra haitanensis） sampled from China. Whereafter, the analyses of probable minimum loci numbers, observed alleles sum, genetic diversity, and unweighted pair-group arithmetic average （UPGMA） cluster were carded out. After initial activity and resolution testing of bands of 23 enzymes, 6 of them （MDH, ME, LDH, GDH, IDH and G-6-PDH） were proved to be appropriate for analysis of the full sample set. The probable minimum numbers of loci and alleles analyses showed that the five species of Porphyra had an extraordinary consistent result in ME loci and alleles. However, P. katadai and P. oligospermatangia differed from other three species of Porphyra in LDH and GDH loci and alleles. P. katadai was independent in the analyses of MDH and P. oligosperTnatangia and P. haitanensis differed from other three species in IDH analyses. Moreover, P. yezoensis and P. haitanensis were apart from other three species in G-6-PDH analysis. Taking one with another, P. katadai was relatively separated in the probable minimum numbers of loci and alleles analyses. The results indicated that the genetic variation among the five Porphyra species was limited with a genetic identity of 0.7550. The hybridize species （P. yezoensis x P. haitanensis） seemed to be high homologue with P. oligospermatangia, unexpectedly got relatively lower average genetic identities with both P. yezoensis and P. haitanensis. The 4 strains of P. yezoensis were relatively divergent with an average genetic identity of 0.7428, and P. katadai presented the most differentiated, compared with other species, which consistented with the result summarized in the probable minimum numbers of loci and alleles analyses.展开更多
This paper presents an advanced and integrated research approach to longwall mining-induced strata move- ment, stress changes, fractures, and gas flow dynamics with actual examples of its application from recent studi...This paper presents an advanced and integrated research approach to longwall mining-induced strata move- ment, stress changes, fractures, and gas flow dynamics with actual examples of its application from recent studies for coextraction of coal and methane development at Huainan Mining Group in China, in a deep and multi-seam mining environment. The advanced approach takes advantage of the latest techniques in Australia for mine scale geotechnical characterisation, field measurement, monitoring and numerical modelling. Key techniques described in this paper include coal mine site 3D geotechnical characterisation methods, surface deep downhole multi-point extensometers and piezometers for overburden displacement and pore pressure measurements during mining, tracer gas tests for goal gas flow patterns, and advanced numerical modelling codes for coupled coal mine strata, water and gas simulations, and longwall goaf gas ttow investigations. This integrated approach has resulted in significant insights into the complex dynamic imeraction between strata, groundwater, and gas during mining at Huainan Mining Group in recent years. Based on the lindings from the extensive field monitoring and numerical modelling studies, a three-dimensional annular-shaped over-lying zone along the perimeter of the longwall panel was identified for optimal methane drainage during mining.展开更多
In view of the relative positioning problem between non-regular quadrilateral grids and regular rectangle grid nodes in the wave front construction method, concrete realization problems with four grid positioning meth...In view of the relative positioning problem between non-regular quadrilateral grids and regular rectangle grid nodes in the wave front construction method, concrete realization problems with four grid positioning methods （vector cross product judgment, angle sum, intersection-point, and signs comparison algorithms） in wave front construction which are commonly used in computer graphics are compared and analyzed in this paper. Based on the stability analysis of the location method, the calculation examples show that the vector cross product judgment method is faster and more accurate than other methods in the realization of the relative positioning between non-regular quadrilateral grids and regular rectangle grid nodes in wave front construction. It provides precise grid point attribute values for the next steps of migration and demigration.展开更多
基金Supported by the National Science Fund for Young Scholars of China (31101598)the Natural Science Fund of Anhui Province, China (090411002)+2 种基金the Youth Science Fund of Anhui Province, China (10040606Y02)the Innovation Team of Corn of Anhui Science and Technology University (2011AKKC2011-1)the Key Disciplines of Anhui Science and Technology University (AKXK2010-1-1)~~
文摘[Objective] This study aimed to analyze the quantitative trait loci （QTLs） of grain storage durability for maize under controlled deterioration conditions by using SSR markers. [Method] The recombinant inbred line population F 2 derived from the cross of shen 137/02-50 was used to do the simple sequence repeats （SSRs） and QTL analysis. [Result] The 2 parents showed good polymorphisms. Three loci were detected on chromosome 1, chromosome 6 and chromosome 9, accounting for 41.2% of total phenotypic. [Conclusion] This study provided theoretical basis for using molecular marker to assist the breeding of storage durable maize varieties.
基金sponsored jointly by the Natural Science Foundation of China (No. 40206019)Agricultural Innovation Project of Nantong (No. L4017)
文摘Vertical polyamide gel electrophoresis was used to investigate isozyme polymorphisms among different isolates （including wild and cultivated） of Porphyra katadai, Porphyra oligospermatangia, Porphyra yezoensis, Porphyra haitanensis, and a hybridize species （Porphyra yezoensis x Porphyra haitanensis） sampled from China. Whereafter, the analyses of probable minimum loci numbers, observed alleles sum, genetic diversity, and unweighted pair-group arithmetic average （UPGMA） cluster were carded out. After initial activity and resolution testing of bands of 23 enzymes, 6 of them （MDH, ME, LDH, GDH, IDH and G-6-PDH） were proved to be appropriate for analysis of the full sample set. The probable minimum numbers of loci and alleles analyses showed that the five species of Porphyra had an extraordinary consistent result in ME loci and alleles. However, P. katadai and P. oligospermatangia differed from other three species of Porphyra in LDH and GDH loci and alleles. P. katadai was independent in the analyses of MDH and P. oligosperTnatangia and P. haitanensis differed from other three species in IDH analyses. Moreover, P. yezoensis and P. haitanensis were apart from other three species in G-6-PDH analysis. Taking one with another, P. katadai was relatively separated in the probable minimum numbers of loci and alleles analyses. The results indicated that the genetic variation among the five Porphyra species was limited with a genetic identity of 0.7550. The hybridize species （P. yezoensis x P. haitanensis） seemed to be high homologue with P. oligospermatangia, unexpectedly got relatively lower average genetic identities with both P. yezoensis and P. haitanensis. The 4 strains of P. yezoensis were relatively divergent with an average genetic identity of 0.7428, and P. katadai presented the most differentiated, compared with other species, which consistented with the result summarized in the probable minimum numbers of loci and alleles analyses.
文摘This paper presents an advanced and integrated research approach to longwall mining-induced strata move- ment, stress changes, fractures, and gas flow dynamics with actual examples of its application from recent studies for coextraction of coal and methane development at Huainan Mining Group in China, in a deep and multi-seam mining environment. The advanced approach takes advantage of the latest techniques in Australia for mine scale geotechnical characterisation, field measurement, monitoring and numerical modelling. Key techniques described in this paper include coal mine site 3D geotechnical characterisation methods, surface deep downhole multi-point extensometers and piezometers for overburden displacement and pore pressure measurements during mining, tracer gas tests for goal gas flow patterns, and advanced numerical modelling codes for coupled coal mine strata, water and gas simulations, and longwall goaf gas ttow investigations. This integrated approach has resulted in significant insights into the complex dynamic imeraction between strata, groundwater, and gas during mining at Huainan Mining Group in recent years. Based on the lindings from the extensive field monitoring and numerical modelling studies, a three-dimensional annular-shaped over-lying zone along the perimeter of the longwall panel was identified for optimal methane drainage during mining.
基金This research work is supported by the Projects of National Science Foundation of China （Grant No, 40574052 and 40437018） and National Basic Research Program of China （973 Program） （Grant No. 2007CB209603）.Acknowledgements We wish to thank Researcher Xu Tao for his advice and comment. We also thank Mrs. Wang Kun for her help in the process of translation.
文摘In view of the relative positioning problem between non-regular quadrilateral grids and regular rectangle grid nodes in the wave front construction method, concrete realization problems with four grid positioning methods （vector cross product judgment, angle sum, intersection-point, and signs comparison algorithms） in wave front construction which are commonly used in computer graphics are compared and analyzed in this paper. Based on the stability analysis of the location method, the calculation examples show that the vector cross product judgment method is faster and more accurate than other methods in the realization of the relative positioning between non-regular quadrilateral grids and regular rectangle grid nodes in wave front construction. It provides precise grid point attribute values for the next steps of migration and demigration.
