鹿茸菇的研究概述

鹿茸菇(Lyophyllum decastes)作为一种珍稀的食药兼用菌,营养丰富且高蛋白低脂肪,具有抗肿瘤、降三高、调节免疫力、保肝护肝等功效,其功能活性物质的主要成分为多糖类、甾醇类及核苷等。对鹿茸菇的驯化栽培、营养价值和活性成分及作用等方面进行详细介绍,为该菌的推广及相关研究提供参考。

Current Situation and Countermeasures of Breeding for Processing Pepper in China

Research progress on the heterosis, cytoplasmic male sterility and biotechnology breeding of processing pepper （Capsicum annuum L.） was introduced, and the problems of limited germplasm resources and varieties of processing pep- per, large gap with the foreign companies were pointed out. Some suggestions were proposed on strengthening innovation and collection of germplasm resources, breed- ing processing pepper varieties, enhancing breeding level of processing pepper and its market competitiveness.

The Preliminary Study on Artificial Propagation and Fry Rearing of Misgurnus anguillicaudatus

[Objective] The aim was to seek the optimal choice of hormones,induced temperature,and initial feed for cultivating loach.[Method] Different hormones were injected to Ioaches for artificial reproduction in water at different temperatures.During cultivation,varied initial feeds were put in to explore the optimal choice of hormones,temperature and initial feed.[Result] According to the test,fecundity of female loach is directly proportional to gonad maturity,namely,the higher gonad maturity,the more fecundity.For example,for a female loach whose maturity coefficient was 24％,absolute and relative fecundity rates were 62 142 and 990.In contrast,for a female whose maturity coefficient was 9％,the rates were 18 207 and 367,respectively.The induced effect differs upon hormones and the test demonstrated that LRH-A2 is better in improving fertility rate compared with HCG.For example,the fertility rate in the group with HCG was 78.84 and in the group with LRH-A2 was 83.04％.It is notable that the mixture of the two at a certain ratio would enhance induced effect and the fertility rate can be as high as 89.17％.With temperature in a certain range,the higher temperature,the better induced effect.The test indicated that water temperature at （30±0.5） ℃ is an optimal one and the effect horizon is 6-8 h.In group 4,induced rate was 93.33％ and the fertility rate was 89.26％.The research indicated that the survival rate was the highest if wheel animalcule,dominated by Brachionus calyciflorus and Brachionus angularis,was taken as an initial feed,followed by artemia nauplii and corpuscule.In addition,loach fry in the group with yolk and wheel animalcule grew about 1.21 times than the group with only yolk,1.04 times than the group with only fairy shrimp,and 1.1 times than the group with fairy shrimp.[Conclusion] The research provides scientific references for scale loach farming.

Breeding of an F_1 Hybrid Cultivar Hangjiao Huangshuai by Space Mutation Breeding Technique

Hangjiao Huangshuai is a processing type yellow pepper F1 hybrid cultivar bred with the inbred line 032-1-2-1-1-H-H bred through space treatment as the female parent and 082-1-1-H-H free of space treatment as the male parent. The fruit is in the shape of long finger with few wrinkles and good gloss, and tastes very spicy .The fruit has a vertical diameter of 17.5 cm, a transverse diameter of 1.6 cm, a fresh thickness of 0,20 cm and a single fruit weight of 15.6 g. It is yellowishgreen in the early maturation stage and yellow in the late maturation stage. The cultivar exhibited high resistance to pepper blight, viral diseases and powdery mildew, and showed an average yield of 56 493,0 kg/hm^2. It could be generalized in Gansu, Ningxia, Liaoning and Zhejiang.

The difference of soluble sugar contents and starch synthetic key enzyme activities between high and low starch cultivar of cassava

The difference of soluble sugar contents and starch synthetic key enzyme activities between high starch cultivar and low starch cultivar were studied in this experiment, of which FuXuan01, GR891, SC124, and SC201 were used as materials. The results showed that the contents of reducing sugar in root tube of low starch cultivars were higher than those of high starch cultivars in all growth periods, the contents of sucrose in root tube of high starch cultivar were higher than those of low starch cultivars in the early growth period, but, lower in the late growth period, and there were almost no difference in the contents of soluble sugar in root tube between high starch cultivar and low starch cultivar in the early growth period, but notable difference in the late growth period and the soluble sugar contents of low starch cultivars were higher than those of high starch cultivars. It also-showed that the activities of ADPGPpase, SSS, and SBE （starch branching enzyme） of high starch cultivars were higher than those of low starch cultivars. It was evident that there were close correlations between the content of sucrose, reducing sugar and soluble sugar in root tube, and the activities of ADPGPpase, SSS, SBE, and the starch accumulation in the root tubers of cassava. It was quite evident in this experiment that the soluble sugar and starch synthetic key enzyme were main factors controlling starch accumulation in root tubers. These results provided important indication for physiological controlling in high starch cultivation and gene engineering breeding of high starch cultivar of cassava.

Plant biotechnology:a case study of Bacillus thuringiensis(Bt) and its application to the future of genetic engineered trees

Agricultural productivity may be raised in a sustainable way by many different technologies such as biological fertilizers, soil and water conservation, biodiversity conservation, improved pest control, and changes in land ownership and distribution. Of these measures, biotechnology applications probably hold the most promise in augmenting conventional agricultural productivity, because biotechnology applications give not only the need to increase production, but also protect the environment and conserving natural resources for future generations. Biotechnology applications will have the possibilities to increase productivity and food availability through better agronomic performance of new varieties, including resistance to pests; rapid multiplication of disease-free plants; ability to obtain natural plant products using tissue culture; diagnosis of diseases of plants and livestock; manipulation of reproduction methods increasing the efficiency of breeding; and the provision of incentives for greater participation by the private sector through investments. Insect resistance through the transfer of a gene for resistance fromBacillus thuringiensis (Bt) is one of the most advanced biotechnology applications already being commercialized in many parts of the world. This paper reviews the development and the status ofBt technology and application ofBt transgenic plants in current agriculture, and discusses specific issues related to the transfer of the technology to the future of genetic engineered trees with emphasis on conifers. Key words Agricultural productivity - Bacillus thuringiensis - Genetic engineering - Insect resistance - Trees CLC number Q812 - S763.306 Document code A Biography: Tang Wei (1964-), male, Ph. Doctor, Research associate, Department of Biology, Howell Science Complex, East Carelina University, Greenville, NC 27858-4353, USA.Responsible editor: Chal Ruihai

A Review on the Identification Indicators of Tea Germplasm

Tea plant is cross pollinated plant, it forms a variety of the tea Germplasm constantly in the long-term breeding process. Tea Germplasm resources are the material foundation for improving varieties and also the basic research which is good for developing and expanding tea well-bred varieties in tea field, especially in quality selection and biotechnology research. Furthermore, excellent tea germplasm is primary condition for tea breeding and quality. Therefore, identification and screening excellent tea germplasm become particularly important. We need accelerate develop high-quality tea varieties, and it need to base on exploring and studying tea germplasm. This paper reviews the research progress of tea germplasm on identification from morphological characteristic, biological characteristic, quality characteristic, resistance and heredity. However, there are still some problems on identification and screening of tea germplasm, no effective methods have been found so far which apply on early identification and genetic engineering, so we should make intensive and long lasting study tea germplasm and analyze systematically various identification, which are benefit for breeding high-quality seeds, shortening the breeding period and summing the new way of breeding. We expect them to play the key roles and provide the theory basis for genetic improvement, classification and breeding in tea plant.

Molecular breeding of hybrid rice

This paper describes in a general way the objectives, contents and methods of molecular breeding, and the application of molecular breeding in hybrid rice.

High-yield Cultivating Techniques of Flammulina velutipes

Flammulina velutipes is an edible fungus with the largest amount of factory-like cultivation yield in China. In this study,based on many years of cultivation experience, 2 kinds of factory-like high-yield cultivating techniques of F. velutipes in China were summarized. One way is bag cultivation, and the other way is bottle cultivation. The cultivation process of the 2 methods were introduced in detail, including mixing material, bagging or bottling, sterilization, inoculation, spawn running, and mushroom fruiting.

Development and application of biological technologies in fish genetic breeding

Fish genetic breeding is a process that remolds heritable traits to obtain neotype and improved varieties.For the purpose of genetic improvement,researchers can select for desirable genetic traits,integrate a suite of traits from different donors,or alter the innate genetic traits of a species.These improved varieties have,in many cases,facilitated the development of the aquaculture industry by lowering costs and increasing both quality and yield.In this review,we present the pertinent literatures and summarize the biological bases and application of selection breeding technologies(containing traditional selective breeding,molecular marker-assisted breeding,genome-wide selective breeding and breeding by controlling single-sex groups),integration breeding technologies(containing cross breeding,nuclear transplantation,germline stem cells and germ cells transplantation,artificial gynogenesis,artificial androgenesis and polyploid breeding)and modification breeding technologies(represented by transgenic breeding)in fish genetic breeding.Additionally,we discuss the progress our laboratory has made in the field of chromosomal ploidy breeding of fish,including distant hybridization,gynogenesis,and androgenesis.Finally,we systematically summarize the research status and known problems associated with each technology.

Current and future editing reagent delivery systems for plant genome editing

Many genome editing tools have been developed and new ones are anticipated; some have been extensively applied in plant genetics, biotechnology and breeding, especially the CRISPR/Cas9 system. These technologies have opened up a new era for crop improvement due to their precise editing of user-specified sequences related to agronomic traits. In this review, we will focus on an update of recent developments in the methodologies of editing reagent delivery, and consider the pros and cons of current delivery systems. Finally, we will reflect on possible future directions.