碱酶一步法水解铬革屑的工艺研究

在原有实验基础上对铬革屑水解工艺参数进行了进一步优化。增加的水洗预处理步骤可以显著降低铬革屑中40%的灰分含量。单因素优化实验结果显示,较佳的工艺参数为氧化钙脱铬温度65℃、氧化钙用量10%、碱处理液比10、氧化钙处理时间30 min、酶水解温度60℃、酶用量0.8%、酶水解时间1.0 h。正交优化实验结果表明,最佳工艺条件为氧化钙脱铬温度68℃、氧化钙用量10%、液比9、碱处理时间25 min、酶水解温度57℃、酶用0.7%、酶水解时间1.0 h,调节水解液pH10.5,静止30min,过滤,弃渣留液。最优工艺方案的水解率为76.79%,铬含量为44.30 mg/kg。最优工艺下胶原蛋白提取率为84.86%,灰分含量8.14%。

Process Optimization of Ultrasonic Extraction of Puerarin Based on Support Vector Machine

In ultrasonic extraction technology, optimization of technical parameters often considers extraction medium only, without including ultrasonic parameters. This paper focuses on controlling the ultrasonic extraction process of puerarin, investigating the influence of ultrasonic parameters on extraction rate, and empirically analyzing the main components of Pueraria, i.e., isoflavone compounds. A method is presented combining orthogonal experi- mental design with a support vector machine and a predictive model is established for optimization of technical parameters. From the analysis with the predictive model, appropriate process parameters are achieved for higher extraction rate. With these parameters in the ultrasonic extraction of puerarin, the experimental result is satisfactory. This method is of significance to the study of extracfing root-stock plant medicines.