巨噬细胞样细胞(MLCs)是一类具有免疫功能的异质性细胞群,主要包括小胶质细胞、玻璃体细胞以及血浆单核-巨噬细胞等。在生理情况下,MLCs参与视网膜稳态维持,在病理情况下则参与视网膜炎性病变,如糖尿病视网膜病变、视网膜静脉阻塞、葡...巨噬细胞样细胞(MLCs)是一类具有免疫功能的异质性细胞群,主要包括小胶质细胞、玻璃体细胞以及血浆单核-巨噬细胞等。在生理情况下,MLCs参与视网膜稳态维持,在病理情况下则参与视网膜炎性病变,如糖尿病视网膜病变、视网膜静脉阻塞、葡萄膜炎等。2020年,CASTANOS M V等首次使用临床光学相干层析成像(OCT)仪器将玻璃体视网膜界面MLCs可视化,至此越来越多的研究小组将其应用于各种视网膜及脉络膜炎性疾病的研究。本研究针对MLCs的一般特征以及其在各种视网膜及脉络膜炎性疾病中的研究进展进行阐述。展开更多
The present study aimed at investigating the possible effects offl-elemene on the progression of atherosclerosis in a rabbit model. The rabbit atherosclerosis model was established by the combination of balloon angiop...The present study aimed at investigating the possible effects offl-elemene on the progression of atherosclerosis in a rabbit model. The rabbit atherosclerosis model was established by the combination of balloon angioplasty-induced endothelial injury and an atherogenic diet fed to the rabbits. New Zealand White rabbits were randomly divided into four groups （8/group）： the normal control group （fed with normal chow diet）, and three experimental groups, placebo group, atorvastatin group, and β-elemene group （received the atherogenic diet）. After two weeks on the diet, the three experimental groups underwent balloon injury at right common carotid artery and were treated with drugs or placebo for five weeks. Serum lipids were measured, Carotid artery lesions were isolated for histological and immunohistochemical analysis. In vitro, RAW264.7 macrophages were pretreated with β-elemene and ox-LDL for 24 h and the viability of macrophages was assayed using the MTT method. TNF-a and IL-6 were also determined. Compared with the control group, the thickness of the atherosclerosis lesion in the placebo group was significantly increased; The thickness the drug treatment groups were significantly decreased, compared with that of the placebo group. The infiltration of macrophage was markedly reduced in the β-elemene group compared with that of the placebo group,β-Elemene treatment also reduced the levels of TC, TC, and LDL-C, compared with the placebo group, β-elemene decreased the TNF-a and IL-6 levels in vitro. In conclusion, our results demonstrated that β-elemene retarded the progression of atherosclerosis in vivo and in vitro, which may be related to the capacity of β-elemene to reduce the infiltration of macrophages and suppress inflammatory factors.展开更多
AMP-activated protein kinase (AMPK) is a sensor of cellular energy status that plays a key role in energetic metabolism regulation.Metabolic changes in immune cells, such as dendritic cell (DC), macrophages, neutrophi...AMP-activated protein kinase (AMPK) is a sensor of cellular energy status that plays a key role in energetic metabolism regulation.Metabolic changes in immune cells, such as dendritic cell (DC), macrophages, neutrophils and lymphocytes that participate in the signal directed programs that promote or inhibit immune mediated diseases, including cancer, atherosclerosis and inflammatory diseases. Multiple pathogenic mechanisms are involved in the initiation and progression of disease, and many pathways have been uncovered. The mechanistic overlap in the metabolic changes and inflammation could indicate that some of the targets they have are in common, whereas AMPK could be useful in treatment of both disorders. The insight into identification of AMPK responsible for specific immune regulation, anti-inflammatory actions and understanding of the underlying molecular mechanism will promote the generation of novel AMPK activators, and provide novel therapy strategy.展开更多
文摘巨噬细胞样细胞(MLCs)是一类具有免疫功能的异质性细胞群,主要包括小胶质细胞、玻璃体细胞以及血浆单核-巨噬细胞等。在生理情况下,MLCs参与视网膜稳态维持,在病理情况下则参与视网膜炎性病变,如糖尿病视网膜病变、视网膜静脉阻塞、葡萄膜炎等。2020年,CASTANOS M V等首次使用临床光学相干层析成像(OCT)仪器将玻璃体视网膜界面MLCs可视化,至此越来越多的研究小组将其应用于各种视网膜及脉络膜炎性疾病的研究。本研究针对MLCs的一般特征以及其在各种视网膜及脉络膜炎性疾病中的研究进展进行阐述。
基金supported by National Science and Technology Infrastructure Program(No.2012BAI30B001)Mega-projects of Science Research for the 12th Five-Year Plan of China(No.2011ZX09401-007)
文摘The present study aimed at investigating the possible effects offl-elemene on the progression of atherosclerosis in a rabbit model. The rabbit atherosclerosis model was established by the combination of balloon angioplasty-induced endothelial injury and an atherogenic diet fed to the rabbits. New Zealand White rabbits were randomly divided into four groups （8/group）： the normal control group （fed with normal chow diet）, and three experimental groups, placebo group, atorvastatin group, and β-elemene group （received the atherogenic diet）. After two weeks on the diet, the three experimental groups underwent balloon injury at right common carotid artery and were treated with drugs or placebo for five weeks. Serum lipids were measured, Carotid artery lesions were isolated for histological and immunohistochemical analysis. In vitro, RAW264.7 macrophages were pretreated with β-elemene and ox-LDL for 24 h and the viability of macrophages was assayed using the MTT method. TNF-a and IL-6 were also determined. Compared with the control group, the thickness of the atherosclerosis lesion in the placebo group was significantly increased; The thickness the drug treatment groups were significantly decreased, compared with that of the placebo group. The infiltration of macrophage was markedly reduced in the β-elemene group compared with that of the placebo group,β-Elemene treatment also reduced the levels of TC, TC, and LDL-C, compared with the placebo group, β-elemene decreased the TNF-a and IL-6 levels in vitro. In conclusion, our results demonstrated that β-elemene retarded the progression of atherosclerosis in vivo and in vitro, which may be related to the capacity of β-elemene to reduce the infiltration of macrophages and suppress inflammatory factors.
基金supported by the Science and Technology Innovation Team of Shanxi Province (201605D131045-18)Key Laboratory of Effective Substances Research and Utilization in Traditional Chinese Medicine of Shanxi Province (201605D111004).
文摘AMP-activated protein kinase (AMPK) is a sensor of cellular energy status that plays a key role in energetic metabolism regulation.Metabolic changes in immune cells, such as dendritic cell (DC), macrophages, neutrophils and lymphocytes that participate in the signal directed programs that promote or inhibit immune mediated diseases, including cancer, atherosclerosis and inflammatory diseases. Multiple pathogenic mechanisms are involved in the initiation and progression of disease, and many pathways have been uncovered. The mechanistic overlap in the metabolic changes and inflammation could indicate that some of the targets they have are in common, whereas AMPK could be useful in treatment of both disorders. The insight into identification of AMPK responsible for specific immune regulation, anti-inflammatory actions and understanding of the underlying molecular mechanism will promote the generation of novel AMPK activators, and provide novel therapy strategy.
