城市空间的文化探索:呼和浩特巨海傲城规划方案

建筑地方化与民族化的研究与实践由来已久,西部大开发政策又为其奠定了新的经济基础.以内蒙古自治区首府呼和浩特市为例,伴随着规模化居住社区开发的兴起,地方化探索也被提升到了城市建设的高度,而且展现出多元化的趋势,包罗了民俗民风、民族形式以及民族色彩等以蒙元文化为核心的多项内容.

巨海新十年:从优秀到卓越

10年向前奔跑,巨海证明了一种可能性:无论外部环境如何变化,市场竞争多么激烈,只要坚持初心,做正确的事,终会迎来自己的星辰与大海。

The Gene of Megalencephalic Leukoencephalopathy with Subcortical Cysts is Mapped on Chromosome 22q13.3 with 250 kb Interval

Objective: Vacuolating megalencephalic leukoencephalopathy with subcortical cysts (MLC) is a recently described syndrome with autosomal recessive mode of inheritance. Its possible gene was located on chromosomal 22q tel with 3-cM. The purpose of this study was to narrow down the genetical distance on chromosomal 22q tel with MLC. Methods: Thirty-nine MLC patients in 33 families were collected,and the linkage analysis and haplotype analysis of twelve informative families were done, using seven microsatellite markers and four SNP markers. Results: The maximum tow-point LOD score for marker 355c18 was 6.65 at recombination fraction 0.02. The haplotype analysis narrowed down the critical region of MLC to 250 kb on chromosomal 22q tel. Conclusion: One of the causing genes of MLC was located on chromosomal 22q tel with 250 kb. Four candidate genes were considered. The heterogeneity of one informative family indicated possible existence of a second locus for MLC.

Spatiotemporal distribution of protozooplankton and copepod nauplii in relation to the occurrence of giant jellyfish in the Yellow Sea

The occurrence of the giant jellyfish, Nemopilema nomurai, has been a frequent phenomenon in the Yellow Sea. However, the relationship between the giant jellyfish and protozoa, in particular ciliates, remains largely unknown. We investigated the distribution of nanoflagellates, ciliates, Noctiluca scintillans, and copepod nauplii along the transect 33~N in the Yellow Sea in June and August, 2012, during an occurrence of the giant jellyfish, and in October of that year when the jellyfish was absent. The organisms studied were mainly concentrated in the surface waters in summer, while in autumn they were evenly distributed in the water column. Nanoflagellate, ciliate, and copepod nauplii biomasses increased from early June to August along with jellyfish growth, the first two decreased in October, while N. scintillans biomass peaked in early June to 3 571 pg C/L and decreased in August and October. In summer, ciliate biomass greatly exceeded that of copepod nauplii （4.61-15.04 ~tg C/L vs. 0.34-0.89 pg C/L）. Ciliate production was even more important than biomass, ranging from 6.59 to 34.19 ~tg C/（L.d） in summer. Our data suggest a tight and positive association among the nano-, micro-, and meso-zooplankton in the study area. Statistical analysis revealed that the abundance and total production of ciliate as well as loricate ciliate biomass were positively correlated with giant jellyfish biomass, indicating a possible predator-prey relationship between ciliates and giant jellyfish. This is in contrast to a previous study, which reported a significant reduction in ciliate standing crops due to the mass occurrence ofN. nomurai in summer. Our study indicates that, with its high biomass and, in particular, high production ciliates might support the mass occurrence of giant jellyfish.

Swells of the East China Sea

Over the past few decades,an increasing number of marine activities have been conducted in the East China Sea,including the construction of various marine structures and the passage of large ships.Marine safety issues are paramount and are becoming more important with respect to the likely increase in size of ocean waves in relation to global climate change and associated typhoons.In addition,swells also can be very dangerous because they induce the resonance of floating structures,including ships.This study focuses on an investigation of swells in the East China Sea and uses hindcast data for waves over the past 5 years in a numerical model,WAVEWATCH III(WW3),together with historical climate data.The numerical calculation domain covers the entire North West Pacific.Next,swells are separated and analyzed using simulated wave fields,and both the characteristics and generation mechanisms of swells are investigated.

Effect of n-3 HUFA levels in rotifers and Artemia on growth and survival of larval black sea bream (Sparus macrocephalus)

Requirement for dietary n-3 HUFA (n-3 highly unsaturated fatty acid) for growth and survival of black sea bream (Sparus macrocephalus) larvae was studied using rotifers and Artemia at various levels of n-3 HUFA. Five treatments with rotifers and Artemia differing in n-3 HUFA were prepared by enriching them with various oil emulsions. Results indicated that dietary n-3 HUFA significantly influence fish n-3 HUFA levels and are essential for growth and survival of black sea bream larvae. The results also indicated that the incorporation of n-3HUFA TG (triacylglycerols) into tissues of larval black sea bream was more effective from natural fish oil in comparison with n-3 HUFA fatty acid ethyl esters from ethyl-esterified oil.

Human Cytomegalovirus Infection Inhibits the Differentiation of Human Hippocampus Neural Precursor Cells into Astrocytes

HCMV is a major cause of congenital brain disease in humans, and its neuropathogenesis is not yet fully understood. The objective of the present study is to investigate the effect of human cytomegalovirus (HCMV) infection on human hippocampus neural precursor cell (NPCs) differentiation in vitro. Fetal hippocampus tissue was dissociated mechanically and then cultured in proliferation medium with EGF and bFGF. The identification and purity of the NPCs were confirmed by using immunofluorescence to detect the expression of the NPCs marker-Nestin. To drive NPCs differentiation, bFGF and EGF were withdrawn from the medium and replaced with FBS (10%). HCMV AD169 (MOI=5) was added into the differentiation medium at the onset of the differentiation. After 7 days of differentiation, in order to confirm whether NPCs are permissive for HCMV infection, immunofluorescence was used to stain for the presence of immediate early (IE) and late (pp65) HCMV proteins in the infected cells. The effects of HCMV infection on NPCs’ differentiation was observed by detecting the ratio of nestin and GFAP positive cells with confocal microscopy and immunofluorescence. The data showed that 95%±8% of the cells (passage 4-8) cultured were Nestin positive which suggested that majority of the cells were NPCs. On day 7 postinfection, most of the infected cells were IE and PP65 positive. The percentage of Nestin-positive cells were 93%±10% and 50%±19% (t=6.03, p<0.01) and those of GFAP-positive cells were 55±17% and 81%±11% (t=3.77, p<0.01) in HCMV treated and control groups respectively. These findings indicate that NPCs are HCMV permissive cells and HCMV (AD 169) infection suppresses the differentiation of Hippocampus-genetic human NPCs into astrocytes. These effects may provide part of the explanation for the abnormalities in brain development associated with congenital HCMV infection.

Fishery Biology of Jumbo Flying Squid Dosidicus gigas off Costa Rica Dome

The jumbo flying squid(Dosidicus gigas) population was surveyed with the help of Chinese squid jigging vessels off the Costa Rica Dome(4°–11°N, 90°–100°W) in 2009 and 2010. The daily catch of D. gigas in the two survey cruises ranged from 0 to 5.5 t and was mostly obtained from the areas bounded by 6°–9°N and 91°–94°W and by 6°30′–7°30′N and 96°–97°W. The sea surface temperature in the areas yielding the most catch ranged from 27.5 to 29℃. The sex ratio of the total catch was 3.75:1(female: male). The mantle length of the squid ranged from 211 to 355 mm(male) and from 204 to 429 mm(female) with an average of 297.9 and 306.7 mm, respectively. In the relationship of the mantle length(mm) and body weight(g) of the squid, there was no significant difference between sexes. The female and male were at a similar maturity, and most individuals are maturing or have matured with a few females being spent. The size(mantle length) and age at the first sexual maturity were 297 mm and 195 d in females, and less than 211 mm and 130 d in males, respectively. Most of the sampled stomachs(70.6%) had no food remains. The major preys of the squids were fish, cephalopods and crustaceans, with the most abundant Myctophum orientale and D. gigas. The preys in more than 65% of the non-empty sampled stomachs evidenced the cannibalism of D. gigas. The results improved current understanding of the fishery biology of D. gigas off the Costa Rica Dome, which may facilitate the assessment and management of relative fishery resources.

Microsatellite Variation in the Oyster Crassostrea plicatula Along the Coast of China

Genetic diversity and differentiation of the oyster Crassostrea plicatula populations from China’s coast were studied based on seven microsatellite loci. All loci showed high polymorphism for all five C. plicatula populations,with an average number of allele per locus of 19.3-27.9 and an average expected heterozygosity of 0.889-0.952. Significant departures from Hardy-Weinberg equilib-rium and deficits of heterozygotes were observed over most populations at each locus,which were fully explained by null alleles. Microsatellite analysis revealed significant subdivision in the C. plicatula populations. According to the neighbor-joining tree con-structed on the basis of the DA distance,the five populations fell into three regional groups,showing a relatively homogeneous genetic structure in geographically close populations. Assignation tests correctly assigned high percentages of individuals to their original populations and groups,and also confirmed the existence of genetic differentiation among C. plicatula populations. The results ob-tained in this study will facilitate the formulation of appropriate fisheries management programs,stock identification and conservation of biodiversity for the species.

Comprehensive analysis of the origin of giant jellyfish near Qinhuangdao in summer

A massive bloom of the giant jellyfi sh Nemopilema nomurai occurred in waters off Qinhuangdao,a port city in Hebei Province,in July 2013.However,jellyfi sh larvae were not found in this location during the previous winter and spring.To determine the possible origin of the giant jellyfi sh medusa in the Bohai Sea,we developed a backward particle-tracking model and a series of numerical simulations were conducted by using the hydrodynamic,three-dimensional Regional Ocean Modeling System(ROMS)results.The simulated results showed that passive particles,representing jellyfi sh medusae,released in surface waters at diff erent dates during the summer had consistent trajectories.Particles released at the sea surface on August1 and 15 could be traced back to the center of the Bohai Sea and to waters between Feiyan Shoal and the new Huanghe(Yellow)River estuary.Particles released on July 1 and 15 could also be traced back to the center of the Bohai Sea and to waters between Feiyan Shoal and only to Zhuangxi tide station.However,none of the particles released in the middle and bottom water layers could be traced back to those areas.Based on the results of the numerical simulations,the distribution characteristics of seafl oor sediments,and observational data for giant jellyfi sh in the region,we suggest that waters between Feiyan Shoal and the new Huanghe River estuary are the likely origin of giant jellyfi sh observed near Qinhuangdao in summer.

Shipping Giant Launches New Line between Tianjin,U.S East Coast

The Grand Alliance group, one of the world＇s shipping giants, has launched a new route betweennorth China＇s port city of Tianjin and the U.S. east coast.

企业兼并 重在机制——红豆集团兼并上海申达摩托车厂的体会

企业兼并重在机制———红豆集团兼并上海申达摩托车厂的体会周海江企业兼并，是一个企业对另一个企业的资产买断，从而获得产权并扩大生产规模。企业兼并又是重在机制，没有好的机制，兼并不会有好的收效。１９９５年冬，红豆集团巨海产业公司的赤兔马摩托车已经上马，但...