Purpose:To study differentially expressed genes in retina of experimental myopic chicken.Methods:Experimental myopia in chicken was induced by form-deprivatin.The mRNA in chicen retina was analyzed by using differenti...Purpose:To study differentially expressed genes in retina of experimental myopic chicken.Methods:Experimental myopia in chicken was induced by form-deprivatin.The mRNA in chicen retina was analyzed by using differential display.Results:Experimental myopia was successfully induced in chicken through form-deprivation.Differentially expressed gene fragments were detected in retina of chicken with myopic evelopment and recovery as compared with normal controld.Conclusion:The differential display of mRNA may be a useful way in cloning myopic-related genes.展开更多
根据DNA元件百科全书计划（encyclopedia of DNA elements,ENCODE）报道显示,人类基因组中能够有效编码蛋白的基因〈2%,其余大部分为非编码基因[1]。非编码基因根据核苷酸长度分为长链非编码RNA（long noncoding RNA,LncRNA）和短链非编...根据DNA元件百科全书计划（encyclopedia of DNA elements,ENCODE）报道显示,人类基因组中能够有效编码蛋白的基因〈2%,其余大部分为非编码基因[1]。非编码基因根据核苷酸长度分为长链非编码RNA（long noncoding RNA,LncRNA）和短链非编码RNA（ncRNA、siRNA、microRNA）。展开更多
The mRNA Differential Display is a new molecular biological strategy for detecting and characterizing altered gene expression in eukaryotic cells which wad developed in 1992.Because of its simplicity,sensitivity and r...The mRNA Differential Display is a new molecular biological strategy for detecting and characterizing altered gene expression in eukaryotic cells which wad developed in 1992.Because of its simplicity,sensitivity and reproducibility,this method should find wide-ranging underpaid application developmental and molecular biology.Therecent successful applications of this method to gene hunting and the technological improvement promise great potential of mRNA Differential Display.展开更多
In searching of differentially expressed genes in human uterine leiomyomas, differential display was used with twelve pairs of primers to compare human uterine leiomyomas with matched myometrium. False positives were ...In searching of differentially expressed genes in human uterine leiomyomas, differential display was used with twelve pairs of primers to compare human uterine leiomyomas with matched myometrium. False positives were eliminated by reverse Northern analysis. Positives were confirmed by Northern blot analysis. RESULTS: Four of 69 cDNA fragments (3 up-regulated named L1, L2 and L3 and 1 down-regulated named M1 in leiomyoma) were confirmed by Northern analysis. Sequence comparison and Northern analysis proved that L1 is exactly the human ribosomal protein S19. It was present ubiquitously in 13 tissues tested but in various levels and even in different size. L1 was highly expressed in parotidean cystadenocarcinoma, pancreatic cancer and breast cancer examined. No mutations have been found in human uterine leiomyomas (n=6). CONCLUSIONS: hRPS19 overexpression might be a universal signal in rapid cell growth tissues.展开更多
Dear Editors,Sexual dimorphism is the systematic difference in size,shape,color,physiology,and behavior,between male and female individuals of the same species（Mei and Gui,2015）.Some studies have indicated that the ...Dear Editors,Sexual dimorphism is the systematic difference in size,shape,color,physiology,and behavior,between male and female individuals of the same species（Mei and Gui,2015）.Some studies have indicated that the traits of sexual dimorphism in vertebrates are the consequences of sex-biased gene expression and are controlled by multiple critical genes during growth and development（Williams and Carroll,2009）.However,the exact molecular mechanism underlying sexual dimorphism remains unclear.展开更多
基金by the Huo Ying-Tong Foundation(Qingjiong Zhang) "2l1" Foundation for Key Subjects (Qingjiong Zhang)
文摘Purpose:To study differentially expressed genes in retina of experimental myopic chicken.Methods:Experimental myopia in chicken was induced by form-deprivatin.The mRNA in chicen retina was analyzed by using differential display.Results:Experimental myopia was successfully induced in chicken through form-deprivation.Differentially expressed gene fragments were detected in retina of chicken with myopic evelopment and recovery as compared with normal controld.Conclusion:The differential display of mRNA may be a useful way in cloning myopic-related genes.
文摘根据DNA元件百科全书计划（encyclopedia of DNA elements,ENCODE）报道显示,人类基因组中能够有效编码蛋白的基因〈2%,其余大部分为非编码基因[1]。非编码基因根据核苷酸长度分为长链非编码RNA（long noncoding RNA,LncRNA）和短链非编码RNA（ncRNA、siRNA、microRNA）。
文摘The mRNA Differential Display is a new molecular biological strategy for detecting and characterizing altered gene expression in eukaryotic cells which wad developed in 1992.Because of its simplicity,sensitivity and reproducibility,this method should find wide-ranging underpaid application developmental and molecular biology.Therecent successful applications of this method to gene hunting and the technological improvement promise great potential of mRNA Differential Display.
文摘In searching of differentially expressed genes in human uterine leiomyomas, differential display was used with twelve pairs of primers to compare human uterine leiomyomas with matched myometrium. False positives were eliminated by reverse Northern analysis. Positives were confirmed by Northern blot analysis. RESULTS: Four of 69 cDNA fragments (3 up-regulated named L1, L2 and L3 and 1 down-regulated named M1 in leiomyoma) were confirmed by Northern analysis. Sequence comparison and Northern analysis proved that L1 is exactly the human ribosomal protein S19. It was present ubiquitously in 13 tissues tested but in various levels and even in different size. L1 was highly expressed in parotidean cystadenocarcinoma, pancreatic cancer and breast cancer examined. No mutations have been found in human uterine leiomyomas (n=6). CONCLUSIONS: hRPS19 overexpression might be a universal signal in rapid cell growth tissues.
基金supported by the State Key Laboratory of Freshwater Ecology and Biotechnology (2015FB03)the Fundamental Research Funds for the Central Universities (52204-12018, 2013PY068)
文摘Dear Editors,Sexual dimorphism is the systematic difference in size,shape,color,physiology,and behavior,between male and female individuals of the same species（Mei and Gui,2015）.Some studies have indicated that the traits of sexual dimorphism in vertebrates are the consequences of sex-biased gene expression and are controlled by multiple critical genes during growth and development（Williams and Carroll,2009）.However,the exact molecular mechanism underlying sexual dimorphism remains unclear.
