Differential display reverse transcription-PCR method was utilized to analyze the differential expression genes in different Arabidopsis ecotypes after inoculating Botrytis cinerea.Total 150 differentially expressed c...Differential display reverse transcription-PCR method was utilized to analyze the differential expression genes in different Arabidopsis ecotypes after inoculating Botrytis cinerea.Total 150 differentially expressed cDNA fragments were obtained,49 cDNA fragments were found to hybridize to cDNA and 5 cDNA fragments were cloned and then sequenced among them.Three fragments displayed high homology(96.1%,99.6% and 100.0%) to phosphatidylinositol transfer-like protein IV(Sec14p protein),TAC clone K9I9 and NADH-dehydrogenase subunit 4L of Arabidopsis thaliana,while alignment with NCBI databases using BLAST program.It was speculated that these fragments might relate to disease-resistance of Arabidopsis to B.cinerea.展开更多
为对犬弓首蛔虫(T.canis)免疫抑制卵巢信息蛋白(Tc-iom)、卵黄原蛋白(Tc-vit)、酪氨酸蛋白激酶(Tctpk)和丝/苏氨酸蛋白磷酸酶(Tc-stp)四个性别相关基因进行差异表达分析。本研究采用SYBR Green I qRT-PCR方法检测Tc-vit、Tc-iom、Tc-tpk...为对犬弓首蛔虫(T.canis)免疫抑制卵巢信息蛋白(Tc-iom)、卵黄原蛋白(Tc-vit)、酪氨酸蛋白激酶(Tctpk)和丝/苏氨酸蛋白磷酸酶(Tc-stp)四个性别相关基因进行差异表达分析。本研究采用SYBR Green I qRT-PCR方法检测Tc-vit、Tc-iom、Tc-tpk、Tc-stp在T.canis雌虫卵巢、输卵管、子宫和雄虫精巢、贮精囊、输精管的转录情况。结果显示,Tc-vit在雌虫卵巢、输卵管和子宫中均有转录,在雄虫精巢和贮精囊中也有转录,但在输精管中无转录;Tc-iom在雌虫卵巢、输卵管和子宫中高水平转录,在雄虫精巢和贮精囊中转录量较低,输精管中无转录;Tc-tpk在雄虫精巢和贮精囊中的转录明显高于其他生殖组织;Tc-stp在雄虫精巢和输精管中转录量较高,在雌虫卵巢、输卵管和子宫中转录量较低。本试验为T.canis性别相关基因的进一步研究奠定了基础。展开更多
文摘Differential display reverse transcription-PCR method was utilized to analyze the differential expression genes in different Arabidopsis ecotypes after inoculating Botrytis cinerea.Total 150 differentially expressed cDNA fragments were obtained,49 cDNA fragments were found to hybridize to cDNA and 5 cDNA fragments were cloned and then sequenced among them.Three fragments displayed high homology(96.1%,99.6% and 100.0%) to phosphatidylinositol transfer-like protein IV(Sec14p protein),TAC clone K9I9 and NADH-dehydrogenase subunit 4L of Arabidopsis thaliana,while alignment with NCBI databases using BLAST program.It was speculated that these fragments might relate to disease-resistance of Arabidopsis to B.cinerea.
文摘为对犬弓首蛔虫(T.canis)免疫抑制卵巢信息蛋白(Tc-iom)、卵黄原蛋白(Tc-vit)、酪氨酸蛋白激酶(Tctpk)和丝/苏氨酸蛋白磷酸酶(Tc-stp)四个性别相关基因进行差异表达分析。本研究采用SYBR Green I qRT-PCR方法检测Tc-vit、Tc-iom、Tc-tpk、Tc-stp在T.canis雌虫卵巢、输卵管、子宫和雄虫精巢、贮精囊、输精管的转录情况。结果显示,Tc-vit在雌虫卵巢、输卵管和子宫中均有转录,在雄虫精巢和贮精囊中也有转录,但在输精管中无转录;Tc-iom在雌虫卵巢、输卵管和子宫中高水平转录,在雄虫精巢和贮精囊中转录量较低,输精管中无转录;Tc-tpk在雄虫精巢和贮精囊中的转录明显高于其他生殖组织;Tc-stp在雄虫精巢和输精管中转录量较高,在雌虫卵巢、输卵管和子宫中转录量较低。本试验为T.canis性别相关基因的进一步研究奠定了基础。
