[Objective] The aim was to obtain the full-length cDNA sequence of Dunaliella parva β-actin gene.[Method] Based on the highly conserved amino acid regions of known β-actin,a pair of degenerate primers was synthesize...[Objective] The aim was to obtain the full-length cDNA sequence of Dunaliella parva β-actin gene.[Method] Based on the highly conserved amino acid regions of known β-actin,a pair of degenerate primers was synthesized to amplify 533 bp cDNA sequences in Dunaliella parva.Then,the 5＇ genomic DNA and 3＇ cDNA sequences were obtained by Genome walking and 3＇-RACE technology based on the obtained sequence.According to the sequences of the 5＇-termini and 3＇-termini,specific primers were synthesized to obtain the full-length cDNA.[Result] The full-length β-actin cDNA included 1 137 bp open reading frame（ORF）,617 bp of 3＇ noncoding region.Similarity analysis indicated that the highest similarity was found between Dunaliella parva and Dunaliella salina.The Dunaliella parva β-actin also showed wide similarity with other algae.[Conclusion] The full-length cDNA sequence of D.parva was firstly obtained,which was highly conserved.展开更多
法国路易十五的情妇蓬巴杜夫人（Madame de Pompadour）说：＂香槟是唯一让女人喝下去变得漂亮的酒。＂巴黎人说：＂香槟是年轻男子在做第一件错事时所喝的酒。＂那可不可以认为＂香槟使女人变得更美丽,所以男子就做了他的第一件错事＂...法国路易十五的情妇蓬巴杜夫人（Madame de Pompadour）说：＂香槟是唯一让女人喝下去变得漂亮的酒。＂巴黎人说：＂香槟是年轻男子在做第一件错事时所喝的酒。＂那可不可以认为＂香槟使女人变得更美丽,所以男子就做了他的第一件错事＂？人一生会遇到约29200个人,两个人相爱的概率是0.000049。一杯125ml,12度的香槟热量约为89卡路里。一个吻会让脸上的29块肌肉处于紧张状态,其中包括12种唇部和17种舌头部位的肌肉,热吻一次消耗12卡路里的热量。展开更多
基金Supported by Comprehensive Strategic Cooperation Project betweenGuangdong Province and Chinese Academy of Sciences (2010A0-90100010)National Key Technology R&D Program of the 12thFive-year Plan of China (2011BAD14B03)Foundation of KeyLaboratory of Renewable Energy and Natural Gas Hydrate,ChineseAcademy of Sciences (y107j6)~~
文摘[Objective] The aim was to obtain the full-length cDNA sequence of Dunaliella parva β-actin gene.[Method] Based on the highly conserved amino acid regions of known β-actin,a pair of degenerate primers was synthesized to amplify 533 bp cDNA sequences in Dunaliella parva.Then,the 5＇ genomic DNA and 3＇ cDNA sequences were obtained by Genome walking and 3＇-RACE technology based on the obtained sequence.According to the sequences of the 5＇-termini and 3＇-termini,specific primers were synthesized to obtain the full-length cDNA.[Result] The full-length β-actin cDNA included 1 137 bp open reading frame（ORF）,617 bp of 3＇ noncoding region.Similarity analysis indicated that the highest similarity was found between Dunaliella parva and Dunaliella salina.The Dunaliella parva β-actin also showed wide similarity with other algae.[Conclusion] The full-length cDNA sequence of D.parva was firstly obtained,which was highly conserved.
文摘法国路易十五的情妇蓬巴杜夫人（Madame de Pompadour）说：＂香槟是唯一让女人喝下去变得漂亮的酒。＂巴黎人说：＂香槟是年轻男子在做第一件错事时所喝的酒。＂那可不可以认为＂香槟使女人变得更美丽,所以男子就做了他的第一件错事＂？人一生会遇到约29200个人,两个人相爱的概率是0.000049。一杯125ml,12度的香槟热量约为89卡路里。一个吻会让脸上的29块肌肉处于紧张状态,其中包括12种唇部和17种舌头部位的肌肉,热吻一次消耗12卡路里的热量。
