Objective. To compare the performances of Papanicolaou test (Pap Test) and of a new liquid-based cytology method, DNA-Citoliq. System (DCS), in a high-ri sk population, with histology confirmation. Methods. Paired s...Objective. To compare the performances of Papanicolaou test (Pap Test) and of a new liquid-based cytology method, DNA-Citoliq. System (DCS), in a high-ri sk population, with histology confirmation. Methods. Paired specimens of exfolia ted cervical cells were collected under split-sample protocol. All patients wer e submitted to colposcopy and a biopsy taken when any atypical transformation zo ne was seen. Sensitivity, specificity, positive and negative predictive values, and overall accuracy of both conventional and DCS methods were computed in relat ion to histology. Results. A total of 1,095 patients were analyzed by two cytol ogy methods and, in 425 (38.8%), histologically. There were significantly more adequate samples with DCS (98.63%) than with conventional (89.6%) smears (P < 0.001). ASCUS was diagnosed significantly more with DCS than with conventional P ap (P < 0.001). Conventional Pap misclassified as normal 55.4%(158/285) of case s with either LSIL or HSIL or cancer at histology, whereas DCS misclassified 31. 2%(89/285) of cases (P < 0.001). DCS had a significantly higher sensitivity (70 %and 91.3%) than the conventional Pap (49.8%and 72.8%) to detect both LSIL+ and HSIL+at histology, respectively. On the other hand, specificity of conventi onal smear (88.2%and 85.2%) was significantly higher than DCS (75.4%and 70.9 %) considering both LSIL+and HSIL+at histology, respectively. Conclusions. Th is study confirms the superiority of the liquid-based cytology system DCS to de tect cervical lesions. The rate of adequate DSC slides was significantly higher than with conventional cytology.展开更多
目的应用薄层液基细胞学技术(Thinprep cytology test,TCT)及TBS(The Bethesda system for reporting cervical cytology,TBS)诊断系统,探讨TCT液基薄层涂片和传统巴氏涂片两种方法在宫颈癌筛查中的敏感性和价值差异,评价TCT在宫颈癌的...目的应用薄层液基细胞学技术(Thinprep cytology test,TCT)及TBS(The Bethesda system for reporting cervical cytology,TBS)诊断系统,探讨TCT液基薄层涂片和传统巴氏涂片两种方法在宫颈癌筛查中的敏感性和价值差异,评价TCT在宫颈癌的预防、诊断和治疗方面的意义。方法回顾分析2007年3月至2009年12月经TCT筛查的15653例涂片和同期29817例传统巴氏涂片,同时以组织活检的诊断结果作为金标准对照。结果 TCT筛查的15653例中上皮细胞异常339例,阳性检出率2.17%,而同期传统巴氏涂片29817例中共检出上皮细胞异常97例,阳性检出率0.33%。结论液基细胞薄层细胞学技术的敏感性和特异性明显高于传统巴氏涂片检测法,对宫颈癌前病变及早期宫颈癌筛查有重要的价值。展开更多
文摘Objective. To compare the performances of Papanicolaou test (Pap Test) and of a new liquid-based cytology method, DNA-Citoliq. System (DCS), in a high-ri sk population, with histology confirmation. Methods. Paired specimens of exfolia ted cervical cells were collected under split-sample protocol. All patients wer e submitted to colposcopy and a biopsy taken when any atypical transformation zo ne was seen. Sensitivity, specificity, positive and negative predictive values, and overall accuracy of both conventional and DCS methods were computed in relat ion to histology. Results. A total of 1,095 patients were analyzed by two cytol ogy methods and, in 425 (38.8%), histologically. There were significantly more adequate samples with DCS (98.63%) than with conventional (89.6%) smears (P < 0.001). ASCUS was diagnosed significantly more with DCS than with conventional P ap (P < 0.001). Conventional Pap misclassified as normal 55.4%(158/285) of case s with either LSIL or HSIL or cancer at histology, whereas DCS misclassified 31. 2%(89/285) of cases (P < 0.001). DCS had a significantly higher sensitivity (70 %and 91.3%) than the conventional Pap (49.8%and 72.8%) to detect both LSIL+ and HSIL+at histology, respectively. On the other hand, specificity of conventi onal smear (88.2%and 85.2%) was significantly higher than DCS (75.4%and 70.9 %) considering both LSIL+and HSIL+at histology, respectively. Conclusions. Th is study confirms the superiority of the liquid-based cytology system DCS to de tect cervical lesions. The rate of adequate DSC slides was significantly higher than with conventional cytology.
文摘目的应用薄层液基细胞学技术(Thinprep cytology test,TCT)及TBS(The Bethesda system for reporting cervical cytology,TBS)诊断系统,探讨TCT液基薄层涂片和传统巴氏涂片两种方法在宫颈癌筛查中的敏感性和价值差异,评价TCT在宫颈癌的预防、诊断和治疗方面的意义。方法回顾分析2007年3月至2009年12月经TCT筛查的15653例涂片和同期29817例传统巴氏涂片,同时以组织活检的诊断结果作为金标准对照。结果 TCT筛查的15653例中上皮细胞异常339例,阳性检出率2.17%,而同期传统巴氏涂片29817例中共检出上皮细胞异常97例,阳性检出率0.33%。结论液基细胞薄层细胞学技术的敏感性和特异性明显高于传统巴氏涂片检测法,对宫颈癌前病变及早期宫颈癌筛查有重要的价值。
