The effects of BTHP on Ca 2+ independent action potential and the two components of delayed rectifier potassium currents were studied in guinea pig single ventricular myocytes by using whole cell patch clamp tec...The effects of BTHP on Ca 2+ independent action potential and the two components of delayed rectifier potassium currents were studied in guinea pig single ventricular myocytes by using whole cell patch clamp technique. BTHP 30 μmol·L -1 significantly prolonged APD 90 from 143±16 ms to 184±21 ms ( P 【0.01, n=5) without affecting either the RP or APA, and the APD prolonging effects of BTHP were independent of extracellular Ca 2+ . BTHP inhibited both I kr (IC 50 =7 9 μmol·L -1 ) and I ks (IC 50 =22 4 μmol·L -1 ) in a concentration dependent fashion. The results demon strated that BTHP had no obvious selectivity for I kr and I ks .展开更多
Objective To identify the protective effect of lipopolysaccharide （LPS） preconditioning against LPS-induced inflammatory damage in dopaminergic neurons of midbrain slice culture and the possible mechanisms. Methods ...Objective To identify the protective effect of lipopolysaccharide （LPS） preconditioning against LPS-induced inflammatory damage in dopaminergic neurons of midbrain slice culture and the possible mechanisms. Methods After cultured in vitro for 14 d, the rat organotypic midbrain slices were pretreated with different concentrations （0, 1, 3, 6 or 10 ng/mL） of LPS for 24 h followed by treatment with 100 ng/mL LPS for 72 h. The whole slice viability was detelmined by measurement of the activity of lactic acid dehydrogenase （LDH）. Tyrosine hydroxylase-immunoreactive （TH-IR） neurons and CD 1 1 b/c equivalent-immunoreactive （OX-42-IR） microglia in the slices were observed by immunohistochemical method, and tumor necrosis factor-α （TNF-α levels in the culture media were detected by enzymelinked immunosorbent assays （ELISA）. Results In the slices treated with 100 ng/mL LPS for 72 h, the number of TH-IR neurons reduced from 191± 12 in the control slices to 46±4, and the LDH activity elevated obviously （P 〈 0.01）, along with remarkably increased number of OX-42-IR cells and production of TNF-α （P 〈 0.01）. Preconditioning with 3 or 6 ng/mL LPS attenuated neuron loss （the number of TH-IR neurons increased to 126± 12 and 180± 13, respectively） and markedly reduced LDH levels （P 〈 0.05）, accompanied by significant decreases of OX-42-IR microglia activation and TNF-α production （P 〈 0.05）. Conclusion Low-dose LPS preconditioning could protect dopaminergic neurons against inflammatory damage in rat midbrain slice culture, and inhibition of microglial activation and reduction of the proinflammatory factor TNF-α production may contribute to this protective effect. Further understanding the underlying mechanism of LPS preconditioning may open a new window for treatment of Parkinson＇s disease.展开更多
Castleman＇s disease （CD） of the pancreas/peripancreas is extremely rare. The recently introduced, endoscopic ultrasonography （EUS）-guided trucut biopsy （TCB） is a useful diagnostic rnodality for obtaining tissu...Castleman＇s disease （CD） of the pancreas/peripancreas is extremely rare. The recently introduced, endoscopic ultrasonography （EUS）-guided trucut biopsy （TCB） is a useful diagnostic rnodality for obtaining tissue samples from peripancreatic lesions. However, its role in diagnosing CD remains unknown. We report a case of localized, peripancreatic, hyaline-vascular CD biopsied using EUS. The pathology results were initially interpreted as an extranodal, marginal-zone B-cell lyrnphoma. However, polyrnerase chain reaction （PCR） study for the IgH gene rearrangement revealed a polyclonal pattern. We also reviewed the relevant literature. To our knowledge, this is the first illustrated report on EUS-TCB findings of CD with its pathology results of EUS-TCB mimicked a B-cell lymphoma.展开更多
AIM: To evaluate the value of CT-guided core-needle biopsy in diagnosis and classification of malignant lymphomas.METHODS: From January 1999 to October 2004, CT-guided core-needle biopsies were performed in 80 patient...AIM: To evaluate the value of CT-guided core-needle biopsy in diagnosis and classification of malignant lymphomas.METHODS: From January 1999 to October 2004, CT-guided core-needle biopsies were performed in 80 patients with suspected malignant lymphoma. Biopsies were performed with an 18-20 G biopsy-cut (CR Bard, Inc., Covington, GA,USA) needle driven by a spring-loaded Bard biopsy gun.RESULTS: A definite diagnosis and accurate histological subtype were obtained in 61 patients with a success rate of 76.25% (61/80). Surgical sampling was performed in 19 patients (23.75%) with non-diagnostic core-needle biopsies. The success rate of CT-guided core-needle biopsy varied with the histopathologic subtypes in our group.The relatively high success rates of core-needle biopsy were noted in diffuse large B-cell non-Hodgkin's lymphoma (NHL, 88.89%) and peripheral T-cell NHL (90%). However,the success rates were relatively low in anaplastic large cell (T/null cell) lymphoma (ALCL, 44.44%) and Hodgkin's disease (HD, 28.57%) in our group.CONCLUSION: CT-guided core-needle biopsy is a reliable means of diagnosing and classifying malignant lymphomas,and can be widely applied in the management of patients with suspected malignant lymphoma.展开更多
From the early days of the moving image and its recordings of facts and events considered to be historical, followed by the consolidation of the classic narrative cinema grammar in the 1910s reaffirmed until today by ...From the early days of the moving image and its recordings of facts and events considered to be historical, followed by the consolidation of the classic narrative cinema grammar in the 1910s reaffirmed until today by a large part of the television production that turns to the past, without disregarding authorial aesthetic experiences produced especially since the 1920s, history has been present for over more than a century in several types of media. Movie theaters, people＇s homes and, nowadays, thanks to new media technology, any and every place are spaces for projecting historical narratives. They are both entertainment--by deploying strategies for constructing a ＂truth＂ about the past--and critical reflection, going against a belief in that possibility, in rendering explicit their nature as a language. Since all of these narratives presuppose an audience, a public, within different genres, styles and formats, with more realist overtones, more to the general public＇s taste, or anti-naturalist, in experiences for smaller audiences, it seems pertinent to discuss these issues considering that audiovisual narratives are powerful agents in constructing a memory of the past. Particularly in this text, we will examine how the most powerful communication enterprise in Brazil - Global Group - had construct a memory of the Brazilian military dictatorship （1964-1985） until 2016.展开更多
The paper proposes to examine aspects of modernity in Cosmopolitan Alexandria in the first half of the 20th century as they are manifested in Jacqueline Carol＇s Cocktails andCamels （1960 and reprinted in 2008）. Wri...The paper proposes to examine aspects of modernity in Cosmopolitan Alexandria in the first half of the 20th century as they are manifested in Jacqueline Carol＇s Cocktails andCamels （1960 and reprinted in 2008）. Written in English by a Lebanese Egyptian, born and raised in Alexandria, the novel investigates issues of cultural and linguistic identity, gender, the rise of national discourse, and the emergence of independent Egypt after the 1952 Revolution through the eyes of a young woman growing up in Cosmopolitan Alexandria as part of the elite but paying attention to all the political and social changes taking place in the city and the whole country展开更多
To achieve a dopamine （DA） response with high sensitivity and high signal-to-noise ratio （S/N） with a patch-clamp system, polypyrrole/graphene （PPy/GR） nanocomposites were steadily electrodeposited by an electro...To achieve a dopamine （DA） response with high sensitivity and high signal-to-noise ratio （S/N） with a patch-clamp system, polypyrrole/graphene （PPy/GR） nanocomposites were steadily electrodeposited by an electrochemical method on a planar mi- croelectrode array （pMEA） fabricated by a standard micromachining process. The electrodeposition process was carried out by chronopotentimetry measurement scanning from 0.1 to 0.8 C/cm2 at the current of 2 mA; 0.5 C/cm2 was found to be optimal. The pMEA modified by PPy/GR at the 0.5 C/cm2 exhibits remarkable properties; for instance, the standard deviation （SD） de- creases from 8.4614×10-al to 5.62×10 11 A, reduced by 33.52%, and the sensitivity increases from 2566.88 to 76114.65 gAmMcm2 , 29.65 times higher than the bare Pt （platinum）. A good linear relationship between the current and DA concentra- tion in the range of 0.30 to 61.71 grn was obtained, with a correlation coefficient of 0.997. The sensor is meaningful for neuro- science research and the treatment of neurological diseases.展开更多
文摘The effects of BTHP on Ca 2+ independent action potential and the two components of delayed rectifier potassium currents were studied in guinea pig single ventricular myocytes by using whole cell patch clamp technique. BTHP 30 μmol·L -1 significantly prolonged APD 90 from 143±16 ms to 184±21 ms ( P 【0.01, n=5) without affecting either the RP or APA, and the APD prolonging effects of BTHP were independent of extracellular Ca 2+ . BTHP inhibited both I kr (IC 50 =7 9 μmol·L -1 ) and I ks (IC 50 =22 4 μmol·L -1 ) in a concentration dependent fashion. The results demon strated that BTHP had no obvious selectivity for I kr and I ks .
基金the Foundation of Beijing Municipal Commission of Education,China (No.200410025011)
文摘Objective To identify the protective effect of lipopolysaccharide （LPS） preconditioning against LPS-induced inflammatory damage in dopaminergic neurons of midbrain slice culture and the possible mechanisms. Methods After cultured in vitro for 14 d, the rat organotypic midbrain slices were pretreated with different concentrations （0, 1, 3, 6 or 10 ng/mL） of LPS for 24 h followed by treatment with 100 ng/mL LPS for 72 h. The whole slice viability was detelmined by measurement of the activity of lactic acid dehydrogenase （LDH）. Tyrosine hydroxylase-immunoreactive （TH-IR） neurons and CD 1 1 b/c equivalent-immunoreactive （OX-42-IR） microglia in the slices were observed by immunohistochemical method, and tumor necrosis factor-α （TNF-α levels in the culture media were detected by enzymelinked immunosorbent assays （ELISA）. Results In the slices treated with 100 ng/mL LPS for 72 h, the number of TH-IR neurons reduced from 191± 12 in the control slices to 46±4, and the LDH activity elevated obviously （P 〈 0.01）, along with remarkably increased number of OX-42-IR cells and production of TNF-α （P 〈 0.01）. Preconditioning with 3 or 6 ng/mL LPS attenuated neuron loss （the number of TH-IR neurons increased to 126± 12 and 180± 13, respectively） and markedly reduced LDH levels （P 〈 0.05）, accompanied by significant decreases of OX-42-IR microglia activation and TNF-α production （P 〈 0.05）. Conclusion Low-dose LPS preconditioning could protect dopaminergic neurons against inflammatory damage in rat midbrain slice culture, and inhibition of microglial activation and reduction of the proinflammatory factor TNF-α production may contribute to this protective effect. Further understanding the underlying mechanism of LPS preconditioning may open a new window for treatment of Parkinson＇s disease.
文摘Castleman＇s disease （CD） of the pancreas/peripancreas is extremely rare. The recently introduced, endoscopic ultrasonography （EUS）-guided trucut biopsy （TCB） is a useful diagnostic rnodality for obtaining tissue samples from peripancreatic lesions. However, its role in diagnosing CD remains unknown. We report a case of localized, peripancreatic, hyaline-vascular CD biopsied using EUS. The pathology results were initially interpreted as an extranodal, marginal-zone B-cell lyrnphoma. However, polyrnerase chain reaction （PCR） study for the IgH gene rearrangement revealed a polyclonal pattern. We also reviewed the relevant literature. To our knowledge, this is the first illustrated report on EUS-TCB findings of CD with its pathology results of EUS-TCB mimicked a B-cell lymphoma.
文摘AIM: To evaluate the value of CT-guided core-needle biopsy in diagnosis and classification of malignant lymphomas.METHODS: From January 1999 to October 2004, CT-guided core-needle biopsies were performed in 80 patients with suspected malignant lymphoma. Biopsies were performed with an 18-20 G biopsy-cut (CR Bard, Inc., Covington, GA,USA) needle driven by a spring-loaded Bard biopsy gun.RESULTS: A definite diagnosis and accurate histological subtype were obtained in 61 patients with a success rate of 76.25% (61/80). Surgical sampling was performed in 19 patients (23.75%) with non-diagnostic core-needle biopsies. The success rate of CT-guided core-needle biopsy varied with the histopathologic subtypes in our group.The relatively high success rates of core-needle biopsy were noted in diffuse large B-cell non-Hodgkin's lymphoma (NHL, 88.89%) and peripheral T-cell NHL (90%). However,the success rates were relatively low in anaplastic large cell (T/null cell) lymphoma (ALCL, 44.44%) and Hodgkin's disease (HD, 28.57%) in our group.CONCLUSION: CT-guided core-needle biopsy is a reliable means of diagnosing and classifying malignant lymphomas,and can be widely applied in the management of patients with suspected malignant lymphoma.
文摘From the early days of the moving image and its recordings of facts and events considered to be historical, followed by the consolidation of the classic narrative cinema grammar in the 1910s reaffirmed until today by a large part of the television production that turns to the past, without disregarding authorial aesthetic experiences produced especially since the 1920s, history has been present for over more than a century in several types of media. Movie theaters, people＇s homes and, nowadays, thanks to new media technology, any and every place are spaces for projecting historical narratives. They are both entertainment--by deploying strategies for constructing a ＂truth＂ about the past--and critical reflection, going against a belief in that possibility, in rendering explicit their nature as a language. Since all of these narratives presuppose an audience, a public, within different genres, styles and formats, with more realist overtones, more to the general public＇s taste, or anti-naturalist, in experiences for smaller audiences, it seems pertinent to discuss these issues considering that audiovisual narratives are powerful agents in constructing a memory of the past. Particularly in this text, we will examine how the most powerful communication enterprise in Brazil - Global Group - had construct a memory of the Brazilian military dictatorship （1964-1985） until 2016.
文摘The paper proposes to examine aspects of modernity in Cosmopolitan Alexandria in the first half of the 20th century as they are manifested in Jacqueline Carol＇s Cocktails andCamels （1960 and reprinted in 2008）. Written in English by a Lebanese Egyptian, born and raised in Alexandria, the novel investigates issues of cultural and linguistic identity, gender, the rise of national discourse, and the emergence of independent Egypt after the 1952 Revolution through the eyes of a young woman growing up in Cosmopolitan Alexandria as part of the elite but paying attention to all the political and social changes taking place in the city and the whole country
基金supported by the National Natural Science Foundation of China(Grant Nos.61125105,61101048,61271147,and 61002037)National Basic Research Program of China("973" Program)(Grant Nos.2011CB933202,2014CB744605)
文摘To achieve a dopamine （DA） response with high sensitivity and high signal-to-noise ratio （S/N） with a patch-clamp system, polypyrrole/graphene （PPy/GR） nanocomposites were steadily electrodeposited by an electrochemical method on a planar mi- croelectrode array （pMEA） fabricated by a standard micromachining process. The electrodeposition process was carried out by chronopotentimetry measurement scanning from 0.1 to 0.8 C/cm2 at the current of 2 mA; 0.5 C/cm2 was found to be optimal. The pMEA modified by PPy/GR at the 0.5 C/cm2 exhibits remarkable properties; for instance, the standard deviation （SD） de- creases from 8.4614×10-al to 5.62×10 11 A, reduced by 33.52%, and the sensitivity increases from 2566.88 to 76114.65 gAmMcm2 , 29.65 times higher than the bare Pt （platinum）. A good linear relationship between the current and DA concentra- tion in the range of 0.30 to 61.71 grn was obtained, with a correlation coefficient of 0.997. The sensor is meaningful for neuro- science research and the treatment of neurological diseases.
