Dear Editor, RNAi has become a mainstream molecular tool for assessing the functions of genes in mammalian cells . Large-scale RNA interference-based analyses are often complicated by false positive and negative hits ...Dear Editor, RNAi has become a mainstream molecular tool for assessing the functions of genes in mammalian cells . Large-scale RNA interference-based analyses are often complicated by false positive and negative hits due to off-target effects and interferon response , which can be attributed at least in part to the use of high concentrations of siRNA. Lowering the amounts of siRNAs and shRNAs can effectively and expediently mitigate the off-target effect and interferon response .展开更多
基金Acknowledgments We thank Jun O Liu from Johns Hopkins School of Medicine for critical discussions and proof-reading of the manuscript. This work was supported by the National Key Project for Basic Research of China（2003CB114403）, National Natural Science Foundation of China （20272029, 20572053, 20421202, 20432010）, Ministry of Education of China （104189） and Nankai University.
文摘Dear Editor, RNAi has become a mainstream molecular tool for assessing the functions of genes in mammalian cells . Large-scale RNA interference-based analyses are often complicated by false positive and negative hits due to off-target effects and interferon response , which can be attributed at least in part to the use of high concentrations of siRNA. Lowering the amounts of siRNAs and shRNAs can effectively and expediently mitigate the off-target effect and interferon response .
