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干斑法保存血浆中HBV、HCV、HIV病毒核酸及其保存效期研究
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作者 张菁 邵春燕 +3 位作者 任苓苓 高玲玲 王明慧 骆群 《中国实验血液学杂志》 CAS CSCD 北大核心 2024年第6期1869-1874,共6页
目的:建立一种以干斑法为基础制作的血液采集卡保存血浆中病毒核酸的方法,通过建立阿伦尼乌斯方程,预测该种保存方法保存核酸的效期,并论证该种保存方法在血站留存血液样本进行核酸复测的可行性。方法:分别将HBV、HCV、HIV核酸阳性的血... 目的:建立一种以干斑法为基础制作的血液采集卡保存血浆中病毒核酸的方法,通过建立阿伦尼乌斯方程,预测该种保存方法保存核酸的效期,并论证该种保存方法在血站留存血液样本进行核酸复测的可行性。方法:分别将HBV、HCV、HIV核酸阳性的血浆样本制备成保存卡,以干浆斑的形式进行保存;将制备的保存卡分别放置于37、45、50和55℃条件下进行加速保存实验,不定期取出各个温度条件下的保存卡片,进行核酸提取操作,纯化出待测核酸样本,进行后续PCR测试,并记录CT值;根据阿伦尼乌斯方程建立起失效时间与储存温度之间的模型,进而推测出血液采集卡在规定保存温度条件下保存核酸的有效期。结果:HBV、HCV、HIV 3种核酸阳性的血浆样本,在使用干斑法保存时,其保存效期与温度的回归方程分别为:y=-11546x+31.74、y=-12949x+36.88、y=-12204x+34.48,线性回归的相关系数r均>0.98。预测HBV、HCV和HIV 3种病毒核酸使用干斑法保存时,在4℃的保存温度下,其保存效期分别为20792、19289和14285 d;在20℃的保存温度下,保存效期分别为2135、1502和1289 d。结论:血液样本中常见的3种病毒核酸,使用干斑法保存时,在加速破坏实验中符合一级反应模式,核酸降解速度与保存的绝对温度之间的关系符合阿伦尼乌斯方程。经过该方程的推算,使用干斑法保存血浆核酸样本,在不高于20℃的保存条件下,核酸稳定性效期最低可达3.5年,基本可满足血站血液样本留样保存年限的要求。 展开更多
关键词 干斑法 阿伦尼乌斯方程 血液采集卡 核酸 稳定性
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Influence of welding parameters on fracture toughness and fatigue crack growth rate in friction stir welded nugget of 2024-T351 aluminum alloy joints 被引量:1
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作者 Danial GHAHREMANI MOGHADAM Khalil FARHANGDOOST 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2016年第10期2567-2585,共19页
Friction stir welding (FSW) was performed on 2024-T351 aluminum alloy plates. Metallographic analysis, Vickers microhardness and XRD tests were conducted to determine the properties of the welded zone. FE simulatio... Friction stir welding (FSW) was performed on 2024-T351 aluminum alloy plates. Metallographic analysis, Vickers microhardness and XRD tests were conducted to determine the properties of the welded zone. FE simulation of the FSW process was implemented for the different welding conditions to extract the residual stress and stress intensity factor (SIF). Fracture and fatigue behaviors of the welds which have the initial crack in the nugget zone and the crack orientation along the welding direction, were studied based on standard test methods. Fracture behavior of the welds was also evaluated by shearography method. The results showed that the tool rotational and traverse speeds affect the fracture toughness and fatigue crack growth rate. FSW provides 18%-49% reductions in maximum fracture load and fracture toughness. A slight diminution in fracture toughness of the joints was observed for lower traverse speed of the tool, and at higher traverse or rotational speeds, increasing the probability of defects may contribute to low fracture toughness. Fatigue crack propagation rate of all welds was slower than that of the base metal for low values of stress intensity factor range ΔK (ΔK〈13 MPa·m^1/2), but is much faster for high values of ΔK. 展开更多
关键词 aluminum alloy friction stir welding fracture toughness SHEAROGRAPHY fatigue crack propagation rate
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Digital speckle pattern interferometric measurement of diffusion coefficients in hydrogels
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作者 周金芳 韩雁 +1 位作者 章献民 徐坚 《Journal of Zhejiang University Science》 EI CSCD 2003年第2期166-169,共4页
The technique of real-time digital speckle pattern interferometry is p roposed to study diffusion of surfactants in hydrogel. The diffusion coefficient is simply and directly determined from the interferograms. An e... The technique of real-time digital speckle pattern interferometry is p roposed to study diffusion of surfactants in hydrogel. The diffusion coefficient is simply and directly determined from the interferograms. An example of diffus ion coefficient measurement of surfactant in agarose gel demonstrates the useful ness of the method. The results obtained are compared with the theoretical simul ating values. 展开更多
关键词 HYDROGEL Diffusion coefficient Speckle pattern in terferometry SURFACTANT
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A sequence pulse counting method for shape measurement in dual-beam speckle interferometry
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作者 LIU Liang LI XiDe 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS 2011年第4期640-646,共7页
A temporal approach to fast shape measurement is presented.In principle,the rotational object method is used in combination with the sequence pulse counting method (SPCM) to determine the height of the object through ... A temporal approach to fast shape measurement is presented.In principle,the rotational object method is used in combination with the sequence pulse counting method (SPCM) to determine the height of the object through calculating the related phase.Two specimens are tested to demonstrate the validity of the approach.One is an object covered by a Chinese character (tea) with a height variety of 0.3 mm,and the other is an object surface with a relatively large fluctuation of 3.5 mm.The experimental results are compared with mechanical measurements.An axis shifting method is also proposed to determine shapes with relatively large fluctuations.Effects of such parameters on the height measurement as incident angle of the dual light beams,tilting angle of the object,and azimuth angle of the measured point are discussed as well. 展开更多
关键词 shape measurement profilometry temporal speckle pattern interferometry phase measurement
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