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提取脂质的改良干柱法
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作者 韩菊 魏福祥 《分析化学》 SCIE EI CAS CSCD 北大核心 2005年第9期1291-1294,共4页
报道了一种提取脂质的改良干柱法。用低毒溶剂正己烷/异丙醇(3∶2,V/V)作洗脱剂代替干柱法中毒性较强的二氯甲烷/甲醇,通过对重要提取条件的考察,建立了改良干柱法提取脂质的最佳条件,并对干柱法提取机理进行了探讨。使用7.5cm×27m... 报道了一种提取脂质的改良干柱法。用低毒溶剂正己烷/异丙醇(3∶2,V/V)作洗脱剂代替干柱法中毒性较强的二氯甲烷/甲醇,通过对重要提取条件的考察,建立了改良干柱法提取脂质的最佳条件,并对干柱法提取机理进行了探讨。使用7.5cm×27mmi.d.玻璃层析柱,内装2.0gCaHPO4·2H2O/Celite545(1∶9),再装入样品、无水Na2SO4、Celite545混合物制备干柱。用40mL正己烷/异丙醇(3∶2,V/V)进行洗脱,可在40min内洗脱完毕。测定了6种肉制品的脂质含量,并做了脂肪酸分析,结果与传统的氯仿/甲醇法一致,统计检验表明,两方法间不存在显著性差异,但改良干柱法洗脱剂毒性更低、试剂用量更少。 展开更多
关键词 改良干柱法 脂质 提取 提取条件 脂质含量 干柱法 改良 NA2SO4 显著性差异 洗脱剂
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干柱法在中草药化学成分分离中的应用创新 被引量:1
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作者 钟方晓 李岩 《时珍国医国药》 CAS CSCD 北大核心 2006年第6期1009-1009,共1页
目的对传统的干柱方法进行了改进。方法采用玻璃干柱代替传统的方法。结果分离效果理想。结论改进后的干柱可快速有效地进行中草药化学成分的分离。
关键词 干柱法 中草药 化学成分 分离
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干柱法测定牛奶中总脂肪含量
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作者 韩菊 魏福祥 罗敏 《食品科技》 CAS 北大核心 2001年第6期54-54,53,共2页
报道了一种测定牛奶中总脂肪含量的快速干柱法。使用7.5cm×27mmi.d玻璃层析柱,以1.5gCelite545/CaHPO4·2H2O(9:1)为非脂成分捕集物,再将样品、无水Na2SO4、Celite545顺序研磨,装入柱中,用35mLCH2Cl2/CH3OH(9:1)进行洗脱,可在2... 报道了一种测定牛奶中总脂肪含量的快速干柱法。使用7.5cm×27mmi.d玻璃层析柱,以1.5gCelite545/CaHPO4·2H2O(9:1)为非脂成分捕集物,再将样品、无水Na2SO4、Celite545顺序研磨,装入柱中,用35mLCH2Cl2/CH3OH(9:1)进行洗脱,可在20min内洗脱完毕。测定了8种市售牛奶的脂肪含量,并与Roese-Gottlieb法进行了对比。结果表明,干柱法不仅操作简便、快速,而且对极性脂提取更完全。 展开更多
关键词 干柱法 牛奶 总脂肪含量 测定
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食品中脂质提取方法研究进展 被引量:11
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作者 韩菊 罗敏 高文惠 《河北工业科技》 CAS 2000年第1期38-40,共3页
对20世纪80年代以来食品中脂质提取测定方法及应用进行了评述,重点介绍了氯仿甲醇法及其改进方法。
关键词 脂质 提取 食品 萃取 干柱法 超临界流体萃取
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Assessing Stylo (Stylosanthes hamata cv. Verano) for Fodder in Semi-arid Nigeria: 1. Effects of Sowing Methods on Growth and Herbage Yield
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《Journal of Agricultural Science and Technology(B)》 2012年第5期519-526,共8页
Field experiments were conducted at Dabagi Farm of the Usmanu Danfodiyo University, Sokoto (13~I'N and 5~15' E), in the Semi-arid of Nigeria, during 2007 and 2008 rainy seasons to determine the effect of sowing me... Field experiments were conducted at Dabagi Farm of the Usmanu Danfodiyo University, Sokoto (13~I'N and 5~15' E), in the Semi-arid of Nigeria, during 2007 and 2008 rainy seasons to determine the effect of sowing methods on growth and fodder yield of Stylosanthes hamata cv. Verano. Three sowing methods (dibbling, broadcasting and drilling) were investigated. The experimental design adopted for the study was the randomized complete block design (RCBD) replicated three times. The parameters monitored included plant height (cm), leaf length (cm), leaf width (cm), number of leaves, canopy spread (cm), stand count and herbage yield. Samples were randomly taken and marked for the measurement of the parameters at 2, 4, 6, 8, 10, 12 and 14 weeks after sowing (WAS). Herbage yield (kg hal) was determined at the end of the 14th week. Result of the study revealed that the sowing methods had significant effect (P 〈 0.05) on growth parameters at 2 to 14 WAS in 2007, 2008 and in their combined analysis. Dry matter yield differed (P 〈 0.05) significantly. Dibbling method produced plants that were taller, longer, with wider canopy spread and leaves as well as more leaves and stands (P 〈 0.05). The year 2007 produced taller plants, longer, wider canopy spread and leaves as well as more leaves and stands (P 〈 0.05) in all the weeks compared to the year 2008. It was therefore concluded from the results of this study that dibbling method of sowing was the best for Stylosanthes hamata cv. Verano production at 50 ~ 50 cm inter and intra-row spacing in the semi-arid of Sokoto agro-ecology in northwestern Nigeria, presumably due to low competition for scarce moisture and creation of more space in this sowing method. 展开更多
关键词 FODDER sowing methods Stylosanthes hamata semi-arid.
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