Purpose: To compare nerve growth factor (NGF) levels in tears and on the ocular surfaces of normal control and non-Sj gren’ s type keratoconjunctivitis sicca subjects,and to investigate the effect of 0.1% prednisol...Purpose: To compare nerve growth factor (NGF) levels in tears and on the ocular surfaces of normal control and non-Sj gren’ s type keratoconjunctivitis sicca subjects,and to investigate the effect of 0.1% prednisolone eyedrops on NGF levels in keratoconjunctivitis sicca patients. Design: Prospective,double-masked,randomized,comparative clinical trial. Participants: Forty-one keratoconjunctivitis sicca patients and 23 age-and gender-matched healthy subjects. Methods: Baseline tear NGF levels were measured in keratoconjunctivitis sicca patients and healthy control subjects using enzyme-linked immunosorbent assays. Keratoconjunctivitis sicca patients received 0.1% prednisolone drops in one eye and 0.1% hyaluronic acid drops in the other,3 times a day for 28 days. Also,impression cytology (IC) and immunostaining for NGF on conjunctival epithelium were performed on both groups. Main Outcome Measures: Tear NGF/total tear protein (TP)-concentration ratio,IC and NGF immunocytologic staining,subjective symptom scale,tear breakup time,and Schirmer values. Results: Keratoconjunctivitis sicca patients were found to have baseline tear NGF concentrations higher than those of age-and gender-matched healthy control subjects (65.9± 14.5 vs. 122.1± 45.3 pg/μ g,P<0.0001). In keratoconjunctivitis sicca patients,prednisolone treatment for 28 days resulted in a decrease in tear NGF levels,symptom scores,and IC scores,whereas hyaluronic acid treatment had no such effect (68.2± 25.0 pg/μ g vs. 108.0± 43.4 pg/μ g,P< 0.0001 for tear NGF/TP ratio; 2.16± 1.01 vs. 3.39± 1.50,P=0.0014 for symptom scale; 1.05± 0.67 vs. 1.61± 0.86,P=0.0317 for IC). Measurements taken at both 14 and 28 days indicated that neither prednisolone nor hyaluronic acid treatment affected breakup time or Schirmer values. Conclusion: Keratoconjunctivitis sicca patients showed elevated levels of tear NGF,which were decreased by treatment with 0.1% prednisolone. These data suggest that ocular surface NGF may play an important role in ocular surface inflammation processes associated with dry eyes.展开更多
目的探讨系统性红斑狼疮(SLE)眼表损害和角膜知觉减退以及其相关性研究。方法采用病例对照研究。观察组为70例(140眼)SLE患者,对照组为62例(124眼)健康体检人群。为了解眼表损害的情况,对两组中每个患者的两眼首先进行泪膜破裂时间测量(...目的探讨系统性红斑狼疮(SLE)眼表损害和角膜知觉减退以及其相关性研究。方法采用病例对照研究。观察组为70例(140眼)SLE患者,对照组为62例(124眼)健康体检人群。为了解眼表损害的情况,对两组中每个患者的两眼首先进行泪膜破裂时间测量(tear film break up time,BUT)、基础泪液分泌试验(Schirmer's I test,sIt)、角膜荧光素染色,然后根据上述3项指标的数据判定干燥性角结膜炎(keratoconjunctivitis sicca,KCS),记录KCS的发生率。测量角膜中央处的知觉。询问合并KCS的SLE患者是否有干眼症状,记录干眼症状的发生率。结果与对照组比较,观察组的SIt值低(t=5.49,P<0.01),BUT值低(t=6.54,P<0.01),角膜荧光素着染阳性率增加(χ2=82.563,P<0.001),KCS发生率高(χ2=16.381,P<0.001)。观察组角膜知觉减退(t=4.492,P<0.001);SLE患者的角膜知觉与SIt、BUT、角膜荧光素染色阳性率有一定的相关关系。合并KCS的SLE患者有干眼症状主诉的角膜知觉高于无干眼症状主诉的,差异有统计学意义(t=3.426,P<0.01)。结论 SLE患者的角膜知觉明显减退,易发生眼表损害。眼表损害严重的KCS患者无眼干不适主诉可能是因为其角膜知觉低下。展开更多
文摘Purpose: To compare nerve growth factor (NGF) levels in tears and on the ocular surfaces of normal control and non-Sj gren’ s type keratoconjunctivitis sicca subjects,and to investigate the effect of 0.1% prednisolone eyedrops on NGF levels in keratoconjunctivitis sicca patients. Design: Prospective,double-masked,randomized,comparative clinical trial. Participants: Forty-one keratoconjunctivitis sicca patients and 23 age-and gender-matched healthy subjects. Methods: Baseline tear NGF levels were measured in keratoconjunctivitis sicca patients and healthy control subjects using enzyme-linked immunosorbent assays. Keratoconjunctivitis sicca patients received 0.1% prednisolone drops in one eye and 0.1% hyaluronic acid drops in the other,3 times a day for 28 days. Also,impression cytology (IC) and immunostaining for NGF on conjunctival epithelium were performed on both groups. Main Outcome Measures: Tear NGF/total tear protein (TP)-concentration ratio,IC and NGF immunocytologic staining,subjective symptom scale,tear breakup time,and Schirmer values. Results: Keratoconjunctivitis sicca patients were found to have baseline tear NGF concentrations higher than those of age-and gender-matched healthy control subjects (65.9± 14.5 vs. 122.1± 45.3 pg/μ g,P<0.0001). In keratoconjunctivitis sicca patients,prednisolone treatment for 28 days resulted in a decrease in tear NGF levels,symptom scores,and IC scores,whereas hyaluronic acid treatment had no such effect (68.2± 25.0 pg/μ g vs. 108.0± 43.4 pg/μ g,P< 0.0001 for tear NGF/TP ratio; 2.16± 1.01 vs. 3.39± 1.50,P=0.0014 for symptom scale; 1.05± 0.67 vs. 1.61± 0.86,P=0.0317 for IC). Measurements taken at both 14 and 28 days indicated that neither prednisolone nor hyaluronic acid treatment affected breakup time or Schirmer values. Conclusion: Keratoconjunctivitis sicca patients showed elevated levels of tear NGF,which were decreased by treatment with 0.1% prednisolone. These data suggest that ocular surface NGF may play an important role in ocular surface inflammation processes associated with dry eyes.
文摘目的探讨系统性红斑狼疮(SLE)眼表损害和角膜知觉减退以及其相关性研究。方法采用病例对照研究。观察组为70例(140眼)SLE患者,对照组为62例(124眼)健康体检人群。为了解眼表损害的情况,对两组中每个患者的两眼首先进行泪膜破裂时间测量(tear film break up time,BUT)、基础泪液分泌试验(Schirmer's I test,sIt)、角膜荧光素染色,然后根据上述3项指标的数据判定干燥性角结膜炎(keratoconjunctivitis sicca,KCS),记录KCS的发生率。测量角膜中央处的知觉。询问合并KCS的SLE患者是否有干眼症状,记录干眼症状的发生率。结果与对照组比较,观察组的SIt值低(t=5.49,P<0.01),BUT值低(t=6.54,P<0.01),角膜荧光素着染阳性率增加(χ2=82.563,P<0.001),KCS发生率高(χ2=16.381,P<0.001)。观察组角膜知觉减退(t=4.492,P<0.001);SLE患者的角膜知觉与SIt、BUT、角膜荧光素染色阳性率有一定的相关关系。合并KCS的SLE患者有干眼症状主诉的角膜知觉高于无干眼症状主诉的,差异有统计学意义(t=3.426,P<0.01)。结论 SLE患者的角膜知觉明显减退,易发生眼表损害。眼表损害严重的KCS患者无眼干不适主诉可能是因为其角膜知觉低下。
