Mycelial growth and yield of two strains ofA. aegerita on different substrates was investigated. Mycelial growth on agar media (PDA, standard, wheat, MEA, CYM, PCA, sawdust extracts: alder and mixture of beech and b...Mycelial growth and yield of two strains ofA. aegerita on different substrates was investigated. Mycelial growth on agar media (PDA, standard, wheat, MEA, CYM, PCA, sawdust extracts: alder and mixture of beech and birch (1:1)) and sawdust substrates (birch, beech, oak, maple, alder) was investigated. Petri dishes (Ф 9 cm) for agar media and biological tubes (18 cm long and Ф 2.5 cm) for sawdust substrates were used. Yield and morphological features were studied on birch, beech and mixture of beech and alder (1:1) sawdust substrates. The temperature of inoculation for agar media and sawdust substrates was 25 ℃. For yielding, when mycelium has completely overgrown the substrate the temperature was decreased to 15-17 ℃ to initiate primordia formation. The cultivation was enlightened 10 h/d with daylight lamps (500 Ix). One crop was harvested after five weeks. The carpophores of black poplar mushrooms were picked up in clusters. There was no statistically important difference between the mycelial growths of the investigated strains. The best growing agar media were PDA (7.3 cm), MEA (7.2 cm) and wheat (7.1 cm), both strains showed the slowest mycelium growth on CYM (5.2 cm). The mycelial growth on sawdust was the best on the beech (7.1 cm) and birch (6.8 cm) sawdust. The best substrate for cultivation ofA. aegerita was mixture of beech and alder sawdust (39.5 g/100 substrate DM). The dry yield of carpophores were the highest on beech and alder substrate (3.2 g/100 substrate DM), but dry matter content was the highest on beech sawdust (15.7%). The heaviest carpophores were harvested from birch sawdust (3.7 g); the biggest caps from beech and alder sawdust (3.3 cm). There were no statistically important differences between the mycelium growth and yielding between both investigated strains.展开更多
As the most diverse metazoan taxa,soil nematodes serve a diversity of functions in soil food webs and thus can regulate microbial community composition and affect organic matter decomposition and nutrient turnover rat...As the most diverse metazoan taxa,soil nematodes serve a diversity of functions in soil food webs and thus can regulate microbial community composition and affect organic matter decomposition and nutrient turnover rates.Because nematodes depend on water flms to access food resources,drought can negatively affect nematode-microbial food webs,yet the impacts of drought on nematode diversity and abundance and how these changes may infuence food web members and their functions are hardly explored.Here,we coupled research along a drought gradient in arid and semiarid grasslands with a detailed intact plant-soil microcosm experiment to explore the patterns and mechanisms of how drought impacts nematode abundance and carbon footprint,microbial phospholipid fatty acid(PLFA)and heterotrophic soil respiration.Overall,in the feld and the microcosm experiments,we found that nematode abundance,carbon footprint and diversity,microbial PLFA and heterotrophic respiration were reduced under drier conditions.In addition,drought altered nematode and microbial community composition,through reducing the nematode channel ratio and increasing the relative fungivorous nematode abundance and the fungal to bacterial ratio.The soil decomposition channel shifted from a bacterial to a fungal pathway in response to drought,indicating decelerated heterotrophic respiration under drought.These results highlight the important contribution of soil nematodes and their associated microbial food web to soil carbon cycling.Our fndings underscore the need to incorporate key soil fauna into terrestrial ecosystem model evaluation.展开更多
文摘Mycelial growth and yield of two strains ofA. aegerita on different substrates was investigated. Mycelial growth on agar media (PDA, standard, wheat, MEA, CYM, PCA, sawdust extracts: alder and mixture of beech and birch (1:1)) and sawdust substrates (birch, beech, oak, maple, alder) was investigated. Petri dishes (Ф 9 cm) for agar media and biological tubes (18 cm long and Ф 2.5 cm) for sawdust substrates were used. Yield and morphological features were studied on birch, beech and mixture of beech and alder (1:1) sawdust substrates. The temperature of inoculation for agar media and sawdust substrates was 25 ℃. For yielding, when mycelium has completely overgrown the substrate the temperature was decreased to 15-17 ℃ to initiate primordia formation. The cultivation was enlightened 10 h/d with daylight lamps (500 Ix). One crop was harvested after five weeks. The carpophores of black poplar mushrooms were picked up in clusters. There was no statistically important difference between the mycelial growths of the investigated strains. The best growing agar media were PDA (7.3 cm), MEA (7.2 cm) and wheat (7.1 cm), both strains showed the slowest mycelium growth on CYM (5.2 cm). The mycelial growth on sawdust was the best on the beech (7.1 cm) and birch (6.8 cm) sawdust. The best substrate for cultivation ofA. aegerita was mixture of beech and alder sawdust (39.5 g/100 substrate DM). The dry yield of carpophores were the highest on beech and alder substrate (3.2 g/100 substrate DM), but dry matter content was the highest on beech sawdust (15.7%). The heaviest carpophores were harvested from birch sawdust (3.7 g); the biggest caps from beech and alder sawdust (3.3 cm). There were no statistically important differences between the mycelium growth and yielding between both investigated strains.
基金supported by the National Natural Science Foundation of China(32371737,32130066,31971454,31971534)Natural Science Foundation of Henan Province(232300420004)Xinyang Academy of Ecological Research Open Foundation(2023DBS10).
文摘As the most diverse metazoan taxa,soil nematodes serve a diversity of functions in soil food webs and thus can regulate microbial community composition and affect organic matter decomposition and nutrient turnover rates.Because nematodes depend on water flms to access food resources,drought can negatively affect nematode-microbial food webs,yet the impacts of drought on nematode diversity and abundance and how these changes may infuence food web members and their functions are hardly explored.Here,we coupled research along a drought gradient in arid and semiarid grasslands with a detailed intact plant-soil microcosm experiment to explore the patterns and mechanisms of how drought impacts nematode abundance and carbon footprint,microbial phospholipid fatty acid(PLFA)and heterotrophic soil respiration.Overall,in the feld and the microcosm experiments,we found that nematode abundance,carbon footprint and diversity,microbial PLFA and heterotrophic respiration were reduced under drier conditions.In addition,drought altered nematode and microbial community composition,through reducing the nematode channel ratio and increasing the relative fungivorous nematode abundance and the fungal to bacterial ratio.The soil decomposition channel shifted from a bacterial to a fungal pathway in response to drought,indicating decelerated heterotrophic respiration under drought.These results highlight the important contribution of soil nematodes and their associated microbial food web to soil carbon cycling.Our fndings underscore the need to incorporate key soil fauna into terrestrial ecosystem model evaluation.