陆基工厂化环境下基于干藻粉投喂马氏珠母贝可行性分析

2023年开始在车间内开展陆基工厂化环境下基于干藻粉投喂马氏珠母贝可行性研究,该研究尝试将海水引入车间,搭建工厂化养殖环境,通过混合投喂多种干藻粉来观察珍珠贝的生长情况。结果显示,以螺旋藻、小球藻为主食,轮虫粉为辅食,养殖3个月的时间,通过3个养殖池的对比试验,存活率最高的二号池能达到72.6%(如果将寒潮期细菌感染死亡数量剔除,存活率能达到82.4%)。表明用干藻粉投喂马氏珠母贝,可大幅降低珍珠养殖成本,能基本解决存活问题。

以干藻粉为原料的MC-LR提取试验研究

针对实验室进行藻毒素(MC)相关研究过程中MC样品昂贵且难以获取这一难题,以太湖水体中的蓝藻干藻粉为研究对象,分别以甲醇、乙醇、乙酸和丁醇-甲醇-水混合溶液为提取剂进行MC提取试验。考察了提取剂种类、干藻粉研磨时间、提取剂体积分数、搅拌时间、微波消解工艺条件对MC提取量的影响。结果表明:4种提取剂都可以提取MC-LR,提取量约为250~300μg/g。而以甲醇为提取剂,研磨90 min,甲醇体积分数为60%,搅拌时间为2 h,微波消解1 min时,MC-LR提取量最多约为300μg/g左右。该方法可为实验室从事藻毒素毒性、化学或生物降解机制等研究工作提供便利,也可为藻泥的商品化提供参考。

雨生红球藻中虾青素的提取工艺优化

[目的]探索大规模工业生产适用的虾青素提取方法。[方法]从原料的状态入手,采用常规的有机溶剂——丙酮作提取剂,对新鲜雨生红球藻膏和干藻粉中虾青素的提取率进行比较。[结果]使用目前采用的提取条件,从干藻粉中得到的虾青素提取率为新鲜藻膏的2倍多。[结论]烘干后的雨生红球藻在虾青素的提取中具有更大的优势。

Comparative experimental study on inhibiting gas explosion using ABC dry powder and diatomite powder

Using a 20 L spherical explosion suppressing test system, the largest gas explosion pressure and maximum pressure rising rate with additives of ultra-fine ABC dry powder and diatomite powder were tested and compared, and the explosion suppression effect of the two kinds of powder was analyzed. Experimental results show that both powders can suppress gas ex- plosion and ABC dry powder is superior to diatomite powder. Adding two powders under the same experimental conditions, when methane concentration is 7.0%, the maximum explosion pressure decreased 39% and 4%, respectively, while the rising rate of the maximum pressure decreased 80% and 53%, respectively. When methane concentration is 9.5%, the maximum ex- plosion pressure decreased 14% and 12%, respectively, the rising rate of maximum pressure decreased 62% and 27%, respec- tively, the maximum explosion pressure decreased 23% and 18%, respectively, while the rising rate of the maximum pressure decreased 77% and 70%, respectively. When methane concentration is 12.0%, the explosion suppression effect of ultra-fine ABC dry powder is not affected by the methane concentration, and the explosion suppression effect of diatomite powder under high methane concentrations is more obvious.

Purification and Antioxidant Activities of Phycocyanin from Spirulina Platensis

This study aimed to purify and determine antioxidant activities of different fractions obtained during the purification process of phycocyanin from Spirulina platensis. The dried powder of Spirulina platensis, after ground with sands, was extracted with 50 mM sodium phosphate buffer at pH 6.8 before centrifuged to precipitate unwanted proteins. Then the supernatant was separated by celit column to obtain semi-pure phycocyanin and further purified by treated with ammonium sulfate. The purity of phycocyanin was monitored by measuring the absorbance spectrum from 200 to 700 nm. Its purity ratio A620A280 was determined. The antioxidant activities of the obtained phycocyanin were determined by 2,2＇-azino-bis（3-ethylbenzthiazoline-6-sulphonic acid） （ABTS） assay and lipid peroxidation （linoleie acid） assay. The purity ratio of phycocyanin in the Spirulina crude extract was 0.36 and increased to 2.68 after purification. The fraction with the highest purity ratio of phycocyanin demonstrated the hightest antioxidant activities. For ABTS assay, it presented the Vitamin C Equivalent Antioxidant Capacity （VCEAC） value of 0.0405 ±0.0002 mg of ascorbic acid/mg of sample and the Trolox Equivalent Antioxidant Capacity （TEAC） value of 0.0485 ±0.0002 mg oftrolox/mg of sample respectively. The result from lipid peroxidation assay exhibited IC50 value of 5.9336 ±0.2565 mg/mL. The purification of Spirulinaplatensis crude extract obtained from this study increased the purity ratio of phycocyanin and its antioxidant activities. This will be further investigated for the development into anti-aging cosmetic products.