OBJECTIVE： To explore the regulatory effects of es- tablished Chinese herbal formulas on inflammatory mediators released during asthma attacks, and to elucidate the molecular mechanism of Traditional Chinese Medicine...OBJECTIVE： To explore the regulatory effects of es- tablished Chinese herbal formulas on inflammatory mediators released during asthma attacks, and to elucidate the molecular mechanism of Traditional Chinese Medicine in the treatment of asthma. METHODS： Seventy-five asthmatic children were randomly divided into a Chinese medication group （45 cases） and a Western medication control group （30 cases）. Patients in the Chinese medication group were treated with a series of established Chi- nese herbal formulas, whereas the Western medica- tion control group received a leukotriene receptor antagonist and a bronchial relaxant. Real-time PCR was used to determine the mRNA expression levels of interleukin （IL）-4, cysteinyl leukotriene receptor 1 （CysLTR1）, and interferon （IFN）-γ in peripheralblood mononuclear cells before and after treat- ment. Enzyme-linked immunosorbent assay was used to measure the peripheral blood levels of IL-4, leukotriene （LTE）-4, and INF-γ before and after treat- ment. RESULTS： After treatment, the mRNA expression levels of 11-4 and CysLTR1 were down-regulated （P〈 0.01） and the mRNA expression levels of IFN-γ were up-regulated （P〈0.05） in the Chinese medication and Western medication groups; no significant dif- ference was found between the two groups. In the Chinese medication group, IL-4 blood level was de- creased and it was significantly different from that in the Western medication group （P〈0.05）; there was also a significant increase in IFN-γ blood levels after treatment with Chinese medica- tion （P〈0.05）. There were no significant differenc- es in LTE-4 blood levels between the two groups before and after treatment （P〉0.05）. CONCLUSION： Chinese medication has a regulato- ry effect on leukotriene receptor gene expression and the imbalance of Th1/Th2 immune cells dur- ing asthma attacks in pediatric patients.展开更多
基金Supported by Major Research Project of Shanghai Traditional Chinese Medicine Three-year Action Plan(ZYSNXD-CC-ZDYJ034)Shanghai Science and Technology Research Grant Program(No.12401905500)Shanghai Health Bureau Medical Research Fund Grant Program(No.2006L032A)
文摘OBJECTIVE： To explore the regulatory effects of es- tablished Chinese herbal formulas on inflammatory mediators released during asthma attacks, and to elucidate the molecular mechanism of Traditional Chinese Medicine in the treatment of asthma. METHODS： Seventy-five asthmatic children were randomly divided into a Chinese medication group （45 cases） and a Western medication control group （30 cases）. Patients in the Chinese medication group were treated with a series of established Chi- nese herbal formulas, whereas the Western medica- tion control group received a leukotriene receptor antagonist and a bronchial relaxant. Real-time PCR was used to determine the mRNA expression levels of interleukin （IL）-4, cysteinyl leukotriene receptor 1 （CysLTR1）, and interferon （IFN）-γ in peripheralblood mononuclear cells before and after treat- ment. Enzyme-linked immunosorbent assay was used to measure the peripheral blood levels of IL-4, leukotriene （LTE）-4, and INF-γ before and after treat- ment. RESULTS： After treatment, the mRNA expression levels of 11-4 and CysLTR1 were down-regulated （P〈 0.01） and the mRNA expression levels of IFN-γ were up-regulated （P〈0.05） in the Chinese medication and Western medication groups; no significant dif- ference was found between the two groups. In the Chinese medication group, IL-4 blood level was de- creased and it was significantly different from that in the Western medication group （P〈0.05）; there was also a significant increase in IFN-γ blood levels after treatment with Chinese medica- tion （P〈0.05）. There were no significant differenc- es in LTE-4 blood levels between the two groups before and after treatment （P〉0.05）. CONCLUSION： Chinese medication has a regulato- ry effect on leukotriene receptor gene expression and the imbalance of Th1/Th2 immune cells dur- ing asthma attacks in pediatric patients.
