黔西北民族地区平织机调查与价值研究

互动式双综双蹑平织机是黔西北分布地区最广、影响范围最大的传统织机,在当地用于原色平纹织物、色织平纹织物与挑花织物的织造。课题组调查了钟山区月照彝族回族苗族乡,六枝特区梭戛苗族彝族回族乡,黔西县林泉镇、红林彝族苗族乡、金坡苗族彝族满族乡、铁石苗族彝族乡、赫章县兴发苗族彝族回族乡、织金县阿弓镇,统计了此类平织机的地理分布,分析了黔西北民族地区平织机通用结构表征,并对研究区域内各地平织机结构进行比较研究,归纳总结了黔西北民族地区平织机在提综装置形态、经面空间分布的异同。在织机结构分析的基础上,梳理了平织机织造三类织物的工艺流程,并对三类织物的使用情况进行分析,归纳其地域性特征。以期能够以点带面,进一步添补我国传统织机的研究空白。

Embryonic hepatocyte transplantation for hepatic cirrhosis:Efficacy and mechanism of action

AIM： To investigate the efficacy and mechanism of action of allogeneic embryonic hepatocyte transplantation for the treatment of hepatic cirrhosis. METHODS： Rat embryonic hepatocytes were characterized by examining cell markers. Wistar rats with CCl4-induced cirrhosis were randomly divided into two groups： a model group receiving continuous CCl4, and a cell transplantation group receiving continuous CCh and transplanted with embryonic fluorescent-labeled hepatocytes. In addition, a normal control group was composed of healthy rats. All rats were sacrificed after 2 wk following the initiation of the cell transplant. Ul- trasound, pathological analyses and serum biochemical tests were used to evaluate the efficacy of embryonic hepatocyte transplantation. To analyze the recovery status of cirrhotic hepatocytes and the signaling pathways influenced by embryonic hepatocyte transplantation, real-time polymerase chain reaction was performed to examine the mRNA expression of stellate activation-associated protein （STAP）, c-myb, ~ smooth muscle actin （^-SMA） and endothelin-1 （ET-1）. West- ern blotting and immunohistochemistry were employed to detect ^-SMA and ET-1 protein expression in hepatic tissues. RESULTS： Gross morphological, ultrasound and his- topathological examinations, serum biochemical tests and radioimmunoassays demonstrated that hepatic cir- rhosis was successfully established in the Wistar rats. Stem cell factor receptor （c-kit）, hepatocyte growth factor receptor （c-Met）, Nestin, ~ fetal protein, albu- min and cytokeratin19 markers were observed in the rat embryonic hepatocytes. Following embryonic hepa- tocyte transplantation, there was a significant reversal in the gross appearance, ultrasound findings, histo- pathological properties, and serum biochemical param- eters of the rat liver. In addition, after the activation of hepatic stellate cells and STAP signaling, ^-SMA, c-myb and ET-1 mRNA levels became significantly lower than in the untreated cirrhotic group （P 〈 0.05）. These levels, however, were not statistically different from those of the normal healthy group. Immunohisto- chemical staining and Western blot analyses revealed that ^-SMA and ET-1 protein expression levels in the transplantation group were significantly lower than in the untreated cirrhotic group, but being not statistically different from the normal group. CONCLUSION： Transplantation of embryonic hepatocytes in rats has therapeutic effects on cirrhosis. The described treatment may significantly reduce the expression of STAP and ET-1.

Microstructure and mechanical properties of AZ31 alloy ingot fabricated by semi-continuous casting

The AZ31 alloy ingot with diameter of 110 mm and length of 3500 mm was fabricated successfully. The compositions and microstructure morphologies of the ingot at different locations were performed, which indicated that the chemical composition distributed homogeneously through the whole alloy ingot and the average grain size increased from the surface to the center. The results of the EDS and element face-scanning illustrated that the eutectic compounds mainly consisted of fl-Mg17Al12 and a small amount of fl-Mgl7（AlZn）12. Furthermore, slight improvements of the strength and ductility were observed from the center to the surface along the axial direction of the alloy ingot, while both the strength and elongation to failure of the samples along the radial direction are higher than that along the axial direction. The fine grain strengthening was the main contributors to the strength of the as-casted AZ31 alloy.

On the construction of the mechanism for the smooth operation of the internal power of the universities

A difficult problem for the system construction of the modem university is the issue how the academic power and the ad- ministrative power can coordinate and orderly operate. The weakening of the academic power and the strengthening of the administrative power make the university slope foot forward in the development. To solve this problem, first of all, we should restore the originality of the academic power and the academic tissues. By perfecting the sla＇uctures and functions of the university organizations, improve the control on the power, and make the power operate in its due orbit, to ensure that the modem university system is not a mere formality.

Research on the Environmental Protection Mechanism under the Framework of WTO

WTO, as the most influencing international economic organization, has given rise to some problems worthy of thinking deeply when creating a great trade platform for all countries. For example, the original ecological environment has been destroyed by the development of trade and economy, so that the countries from all over the world are encountering serious environmental crisis. In this paper, the current situation under the new economic environment is introduced first, the environmental protection mechanism in the report of the WTO panel, and also comprehensive countermeasures performed in the new environmental protection mechanism are proposed. Hopefully, this paper can play a help in the further researches.

Defect-related luminescent bur-like hydroxyapatite microspheres induced apoptosis of MC3T3-E1 cells by lysosomal and mitochondrial pathways

When orthopedic joints coated by hydroxyapatite(HA) were implanted in the human body, they release wear debris into the surrounding tissues. The generation and accumulation of wear particles will induce aseptic loosening. However, the potential bioeffect and mechanism of HA-coated orthopedic implants on bone cells are poorly understood. In this study, defect-related luminescent bur-like hydroxyapatite(BHA) microspheres with the average diameter of 7–9 μm which are comparable to that of the wear-debris particles from aseptically loosened HA implants or HA debris have been synthesized by hydrothermal synthesis and the MC3 T3-E1 cells were set as a cells model to study the potential bioeffect and mechanism of BHA microspheres. The studies demonstrated that BHA microspheres could be taken into MC3 T3-E1 cells via endocytosis involved in micropinocytosisand clathrin-mediated endocytosis process, and exert cytotoxicity effect. BHA microspheres could induce the cell apoptosis by intracellular production of reactive oxygen species(ROS), which led to not only an increase in the permeability of lysosome and release of cathepsins B, but also mitochondrial dysfunction and DNA damage. Our results provide novel evidence to elucidate their toxicity mechanisms and might be helpful for more reasonable applications of HA-based orthopaedic implants in the future.

Clinical analysis of 54 cases of large area soft tissue avulsion in the lower limb

Objective： To assess the clinical curative effect of different treatment methods for large area avulsion injury in the lower limb. Methods： Between January 2010 and December 2013, 54 patients with large area avulsion injury in the lower limb were treated in the trauma center of our hospital, including 34 males and 20 females with a mean age of 35.7 years （range, 16-65 years）. The injury mechanism was traffic accident in 44 cases, hitting by heawj objects in 8 cases, and fall from height in 2 cases, involving 31 thighs, 19 legs and 4 feet involved. The sizes of the avulsed wounds ranged from 10 cm × 15 cm to 50 crn × 30 cm. There were 16 cases combined with hemorrhagic shock, 5 with femoral fractures, and 7 with tibiofibula fractures. Averagely the patients were sent to our hospital within 3.5 h （range, 1.5-10 h） after injury. For the 54 patients, three different surgical strategies were performed based on the wound area and condition of the avulsed skin： in Group A, 24 patients were treated by debridement and preservation of subcutaneous vascular network ＋ vertical mattress suture of full thickness skin flap ＋ tube drainage; in Group B, 25 patients were treated by split-thickness skin flap meshing and grafting ＋ vacuum sealing drainage （VSD）; and in Group C, the other 5 patients were treated by debridement and VSD at stage I ＋ reattachment of autologous reserved frozen split-thickness skin graft at stage II. Results： All the 54 patients recovered and were discharged eventually, without any deaths or amputees. In each group, there were no statistical differences （all p 〉 0.05） among different injury sites in terms of survival rate and length of hospital stay, except for the infection rate, which was much higher （p - 0.000） at the leg area than that at the thigh （32.54%± 2.97% vs. 2.32% ±2.34% in Group A and 50.00%±0.00% vs. 0 in Group C） or the foot （50.00% ±0.00% vs. 0 in Group C）. Moreover comparison of the three surgical methods showed a significant different （all p 〈 0.05） between each other for all the three assessed parameters, i.e. flap survival rate, length of hospital stay, and infection rate. Conclusion： Treatment choices for skin avulsion on the lower limb should be based on the viability of the avulsed skin flap and the location of the wound. Proper choice can not only reduce the economic burden caused by using VSD, but also shorten the long hospital stay due to repeated wound dressing change or second stage surgery.