Aim To establish a LC-MS method for determining the concentration of nifedipine in human plasma and to evaluate the pharmacokinetic characteristics of nifedipine sustained-release tablets. Methods A XB-C18 （5 μm, 4....Aim To establish a LC-MS method for determining the concentration of nifedipine in human plasma and to evaluate the pharmacokinetic characteristics of nifedipine sustained-release tablets. Methods A XB-C18 （5 μm, 4.6 mm ×150 mm） column and a mobile phase of methanol： 0.01 mol·L^-1ammonium acetate （60：40, V/V） were used to separate nifedipine, the detections was accuracy under atmosperic pressure electronic spray ionization （AP-ESI） mode and ion mass spectrum （m/z） of 314.9 [M＋H]^＋ for nifedipine, and 320.8 [M＋H]^＋ for lorazepam （Internal Standard, IS）. Results The linear range of nifedipine was 0.3 - 80 ng·mL^-1 （ r = 0.9997）, and the limit of quantitation （LOQ） was 0.3 ng·mL^-1. The nifedipine pharmacokinetic parameters after a single dose of 20 mg nifedipine sustained-release tablets test （T） or reference （R） were as the followings, t1/2 （6.73 ± 2.00） h and （7.04 ± 2.18） h, Tmax （4.28 ± 0.70） h and （4.48 ± 0.70） h, Cmax（39.66 ± 10.58） ng·mL^-1 and （40.19 ± 10.97） ng·mL^-1, AUC0-36 （391.63 ± 108.55） ng·mL^-1·h and （387.57 ± 121.51） ng·mL^-1·h, and AUC0-∞ （408.28 ± 121.16） ng·mL^-1·h and （406.15 ± 133.13） ng·mL^-1·h. The relative bioavailability of nifedipine sustained-release tablets （test） was （103.02 ± 13.93） %. Conclusion LC-MS method for the determination of concentrations of nifedipine in human plasma was sensitive and accurate, and could be used in nifedipine bioavailability and pharmacokinetic studies.展开更多
Aim A novel method has been developed for evaluation of the levels of total residual protein in antibiotics produced by fermentation using gel filtration chromatography (GFC) combined with Bradford assay based on dete...Aim A novel method has been developed for evaluation of the levels of total residual protein in antibiotics produced by fermentation using gel filtration chromatography (GFC) combined with Bradford assay based on determination of residual protein in lincomycin hydrochloride. Methods The chromatographic conditions were SuperdexTM peptide column, 0.01 mol*L-1 phosphate buffer solution as mobile phase, and flow rate of 1 mL·min-1. Five hundred microliters of lincomycin hydrochloride solution (3 g of lincomycin hydrochloride dissolved in 10 mL of mobile phase) was injected into the chromatograph and the eluted solution was collected between 6 min and 14.5 min (protein eluted from column within this period), and the residual content of total protein in the eluted solution was assayed using Bradford assay method. Results The average recovery was more than 90% for bovine serum albumin, the calibration equation for the range of 0-12 μg·mL-1 of protein was y=-0.002 4x2+0.064 2x+0.002 9, r2=0.999 9, RSD=0.1%-0.9%, and the LOD and LOQ were 3 and 10 ng·mL-1 of protein, respectively. Conclusion The novel method for determining the residual protein in ferment antibio-tics is simple, rapid, and precise.展开更多
A novel approach for fruit shape detection in RGB space was proposed,which was based on fast level set and Chan-Vese model named as Modified Chan-Vese model(MCV) . This new algorithm is fast and suitable for fruit sor...A novel approach for fruit shape detection in RGB space was proposed,which was based on fast level set and Chan-Vese model named as Modified Chan-Vese model(MCV) . This new algorithm is fast and suitable for fruit sorting because it does not need re-initializing. MCV has three advantages compared to the traditional methods. First,it provides a unified frame-work for detecting fruit shape boundary,and does not need any preprocessing even though the raw image is noisy or blurred. Second,if the fruit has different colors at the edges,it can detect perfect boundary. Third,it processed directly in color space without any transformations that may lose much information. The proposed method has been applied to fruit shape detection with promising result.展开更多
Laboratory experiments and field observations show that the equilibrium range of wind wave spectra presents a – 4 power law when it is scaled properly. This feature has been attributed to energy balance in spectral s...Laboratory experiments and field observations show that the equilibrium range of wind wave spectra presents a – 4 power law when it is scaled properly. This feature has been attributed to energy balance in spectral space by many researchers. In this paper we point out that white noise on an oscillation system can also lead to a similar inverse power law in the corresponding displacement spectrum, implying that the – 4 power law for the equilibrium range of wind wave spectra may probably only reflect the randomicity of the wind waves rather than any other dynamical processes in physical space. This explanation may shed light on the mechanism of other physical processes with spectra also showing an inverse power law, such as isotropic turbulence, internal waves, etc.展开更多
A field experiment was carried out to study nutrient balance among N, P, K and Mg in potato cultivation on an acid purple soil in Chongqing, China. The experiment included 8 treatments with equal P rate of 120 kg P2O5...A field experiment was carried out to study nutrient balance among N, P, K and Mg in potato cultivation on an acid purple soil in Chongqing, China. The experiment included 8 treatments with equal P rate of 120 kg P2O5 hm-2: N0K2, N1K2, N2K2, N3K2, N2K0, N2K1, N2K1Mg and N2K3, where N0, N1, N2 and N3 stand for the N rates of 0, 75, 150 and 225 kg N hm-2, and K0, K1, K2 and K3 for the K rates of 0, 165, 330 and 495 kg K2O hm-2, respectively. Among the treatments designed, Thatment N2K2 with a nutrient supply ratio of N:P2O5:K2O:MgO = 1.25:1:2.75:0.28 gave the highest tuber yield and dry matter, highest starch and Zn and lowest NO3- contents in tuber, and high N, P and K use efficiency with an uptake ratio of N:P:K:Mg = 11.38:1:13.32:0.33 by tuber. Yield and starch and protein contents of tuber were the lowest in Treatment N0K2. Dry matter was the lowest but protein and NO3- contents were the highest in Treatment N2K0. Treatment N2K1Mg had the highest N, P and K utilization rates. Statistical analysis showed that yields of tuber and starch were in a positive linear correlation with the uptake amount of various nutrients and protein of the potato tuber was in a significantly positive linear correlation with tuber N cotent and in a significantly negative linear correlation with tuber K and Mg contents. Balanced application of N, P, K and Mg fertilizers (Treatmeat N2K2) was recommended for realization of high yield and good quality in potato cultivation.展开更多
Objective： The aim of the research was to study the clinical and epidemiological characteristics of incidence of colorectal cancer in North China. Methods： Analysis and summary were made for 704 colorectal cancer pa...Objective： The aim of the research was to study the clinical and epidemiological characteristics of incidence of colorectal cancer in North China. Methods： Analysis and summary were made for 704 colorectal cancer patients diagnosed from 2004 to 2008 in North China. Results： （1） Of 704 colorectal cancer patients, the median age was 56 years old, and the mean age was 58.2. There was no statistic difference in age between male and female cases, P 〉 0.05. （2） The male-female ratio was 1.62：1. （3） The proportion of rectal cancer was 62.92%, and incidence of the left colon cancer was higher than that of right colon cancer, P 〈 0.05. （4） Of 712 lesions, 658 cases were adenocarcinoma accounting for 92.4%, 34 were malignant adenoma accounting for 4.8%, and 20 carcinoid accounting for 2.8%. （5） The 48.9% of patients belonged to stages 0 to 11B. Conclusion： The cases of colorectal cancer in North China is mainly rectal cancers, and the incidence of left colon cancer is higher than that of right colon cancer. The histopathological classification is mainly adenocarcinoma, and the ratio of early stage is high.展开更多
Broodstock of Verasper moseri （Jordan and Gilbert） aged 3-4 years old were selected, and reinforced cultivation was conducted to promote maturation under controlled water temperature and photoperiod conditions. Fert...Broodstock of Verasper moseri （Jordan and Gilbert） aged 3-4 years old were selected, and reinforced cultivation was conducted to promote maturation under controlled water temperature and photoperiod conditions. Fertilized eggs were obtained by artificial fertilization, and the development of embryos, larvae and juveniles was observed continuously. The results showed that the fertilized eggs of V. moseri were spherical, with transparent yolk and homogeneous bioplasm, and had no oil globule inside. The average diameter of the eggs was 1.77＋0.02 mm. The eggs of V. moseri were buoyant in water with salinity above 35. The cleavage type was typical discoidal. Young pigment cells appeared when olfactory plates began to form. Hatching occurred at 187 h after fertilization at a water temperature of 8.5℃. The newly hatched larvae, floating on the water surface, were transparent with an average total length of 4.69±0.15 mm. During the cultivation period, when the water temperature was raised from 9 to 14.5℃, 4-day old larvae showed more melanophores on the body surface, making the larvae gray in color. The pectoral fins began to develop, which enabled the larvae to swim horizontally and in a lively manner. On days 7-8, the digestive duct formed. The yolk sac was small and black. The yolk sac was absorbed on day 11. Larvae took food actively, and body length and body height clearly increased. The rudiments of dorsal and anal fin pterygiophores were discernible and caudal fin ray elements formed on day 19. On day 24, the larval notochord flexed upwards, and the rays of unpaired fins began to differentiate. Pigment cells converged on the dorsal and anal fin rays, and the mastoid teeth on the mandible appeared. On day 29, the left eyes of juveniles began to move upwards. Depigmentation began in some juveniles and they became sandy brown in color on day 37. Most juveniles began to settle on the bottom of the tank. The left eyes of juveniles migrated completely to the right side on day 50, when the average body length attained 2.5±0.18 cm, and juveniles accomplished metamorphosis to young. The embryonic and larval characters of several flounder species are compared.展开更多
A single-mode optical fiber with a convex chromatic dispersion profile is proposed for generating a flat supercontinuum(SC).The fiber has normal dispersion and the dispersion parameter D(λ,z) is a convex function of ...A single-mode optical fiber with a convex chromatic dispersion profile is proposed for generating a flat supercontinuum(SC).The fiber has normal dispersion and the dispersion parameter D(λ,z) is a convex function of wavelengths.It is shown from the numerical results that the chromatic dispersion,the flatness of the dispersion curve and the pump conditions have significant effect on SC generation.A flat and broad SC without strong residual pump component can be obtained when the pump wavelength is set in the vicinity of the wavelength at which the fiber has small normal group-velocity dispersion(GVD) and small dispersion slope.The fiber with a smaller normal GVD,a flatter dispersion profile and a higher nonlinear coefficient are more suitable for broad SC generation.展开更多
文摘Aim To establish a LC-MS method for determining the concentration of nifedipine in human plasma and to evaluate the pharmacokinetic characteristics of nifedipine sustained-release tablets. Methods A XB-C18 （5 μm, 4.6 mm ×150 mm） column and a mobile phase of methanol： 0.01 mol·L^-1ammonium acetate （60：40, V/V） were used to separate nifedipine, the detections was accuracy under atmosperic pressure electronic spray ionization （AP-ESI） mode and ion mass spectrum （m/z） of 314.9 [M＋H]^＋ for nifedipine, and 320.8 [M＋H]^＋ for lorazepam （Internal Standard, IS）. Results The linear range of nifedipine was 0.3 - 80 ng·mL^-1 （ r = 0.9997）, and the limit of quantitation （LOQ） was 0.3 ng·mL^-1. The nifedipine pharmacokinetic parameters after a single dose of 20 mg nifedipine sustained-release tablets test （T） or reference （R） were as the followings, t1/2 （6.73 ± 2.00） h and （7.04 ± 2.18） h, Tmax （4.28 ± 0.70） h and （4.48 ± 0.70） h, Cmax（39.66 ± 10.58） ng·mL^-1 and （40.19 ± 10.97） ng·mL^-1, AUC0-36 （391.63 ± 108.55） ng·mL^-1·h and （387.57 ± 121.51） ng·mL^-1·h, and AUC0-∞ （408.28 ± 121.16） ng·mL^-1·h and （406.15 ± 133.13） ng·mL^-1·h. The relative bioavailability of nifedipine sustained-release tablets （test） was （103.02 ± 13.93） %. Conclusion LC-MS method for the determination of concentrations of nifedipine in human plasma was sensitive and accurate, and could be used in nifedipine bioavailability and pharmacokinetic studies.
文摘Aim A novel method has been developed for evaluation of the levels of total residual protein in antibiotics produced by fermentation using gel filtration chromatography (GFC) combined with Bradford assay based on determination of residual protein in lincomycin hydrochloride. Methods The chromatographic conditions were SuperdexTM peptide column, 0.01 mol*L-1 phosphate buffer solution as mobile phase, and flow rate of 1 mL·min-1. Five hundred microliters of lincomycin hydrochloride solution (3 g of lincomycin hydrochloride dissolved in 10 mL of mobile phase) was injected into the chromatograph and the eluted solution was collected between 6 min and 14.5 min (protein eluted from column within this period), and the residual content of total protein in the eluted solution was assayed using Bradford assay method. Results The average recovery was more than 90% for bovine serum albumin, the calibration equation for the range of 0-12 μg·mL-1 of protein was y=-0.002 4x2+0.064 2x+0.002 9, r2=0.999 9, RSD=0.1%-0.9%, and the LOD and LOQ were 3 and 10 ng·mL-1 of protein, respectively. Conclusion The novel method for determining the residual protein in ferment antibio-tics is simple, rapid, and precise.
基金Project supported by the National Natural Science Foundation ofChina (No. 30671197)the Program for New Century ExcellentTalents in University (No. NCET-04-0524), China
文摘A novel approach for fruit shape detection in RGB space was proposed,which was based on fast level set and Chan-Vese model named as Modified Chan-Vese model(MCV) . This new algorithm is fast and suitable for fruit sorting because it does not need re-initializing. MCV has three advantages compared to the traditional methods. First,it provides a unified frame-work for detecting fruit shape boundary,and does not need any preprocessing even though the raw image is noisy or blurred. Second,if the fruit has different colors at the edges,it can detect perfect boundary. Third,it processed directly in color space without any transformations that may lose much information. The proposed method has been applied to fruit shape detection with promising result.
基金This study was financially supported by the National Natural Science Foundation of China （Grant No. 40406008）the Foundation for 0pen Projects of the Key Lab of Physical 0ceanography, the Ministry of Education, China （Grant No. 200309）.
文摘Laboratory experiments and field observations show that the equilibrium range of wind wave spectra presents a – 4 power law when it is scaled properly. This feature has been attributed to energy balance in spectral space by many researchers. In this paper we point out that white noise on an oscillation system can also lead to a similar inverse power law in the corresponding displacement spectrum, implying that the – 4 power law for the equilibrium range of wind wave spectra may probably only reflect the randomicity of the wind waves rather than any other dynamical processes in physical space. This explanation may shed light on the mechanism of other physical processes with spectra also showing an inverse power law, such as isotropic turbulence, internal waves, etc.
文摘A field experiment was carried out to study nutrient balance among N, P, K and Mg in potato cultivation on an acid purple soil in Chongqing, China. The experiment included 8 treatments with equal P rate of 120 kg P2O5 hm-2: N0K2, N1K2, N2K2, N3K2, N2K0, N2K1, N2K1Mg and N2K3, where N0, N1, N2 and N3 stand for the N rates of 0, 75, 150 and 225 kg N hm-2, and K0, K1, K2 and K3 for the K rates of 0, 165, 330 and 495 kg K2O hm-2, respectively. Among the treatments designed, Thatment N2K2 with a nutrient supply ratio of N:P2O5:K2O:MgO = 1.25:1:2.75:0.28 gave the highest tuber yield and dry matter, highest starch and Zn and lowest NO3- contents in tuber, and high N, P and K use efficiency with an uptake ratio of N:P:K:Mg = 11.38:1:13.32:0.33 by tuber. Yield and starch and protein contents of tuber were the lowest in Treatment N0K2. Dry matter was the lowest but protein and NO3- contents were the highest in Treatment N2K0. Treatment N2K1Mg had the highest N, P and K utilization rates. Statistical analysis showed that yields of tuber and starch were in a positive linear correlation with the uptake amount of various nutrients and protein of the potato tuber was in a significantly positive linear correlation with tuber N cotent and in a significantly negative linear correlation with tuber K and Mg contents. Balanced application of N, P, K and Mg fertilizers (Treatmeat N2K2) was recommended for realization of high yield and good quality in potato cultivation.
文摘Objective： The aim of the research was to study the clinical and epidemiological characteristics of incidence of colorectal cancer in North China. Methods： Analysis and summary were made for 704 colorectal cancer patients diagnosed from 2004 to 2008 in North China. Results： （1） Of 704 colorectal cancer patients, the median age was 56 years old, and the mean age was 58.2. There was no statistic difference in age between male and female cases, P 〉 0.05. （2） The male-female ratio was 1.62：1. （3） The proportion of rectal cancer was 62.92%, and incidence of the left colon cancer was higher than that of right colon cancer, P 〈 0.05. （4） Of 712 lesions, 658 cases were adenocarcinoma accounting for 92.4%, 34 were malignant adenoma accounting for 4.8%, and 20 carcinoid accounting for 2.8%. （5） The 48.9% of patients belonged to stages 0 to 11B. Conclusion： The cases of colorectal cancer in North China is mainly rectal cancers, and the incidence of left colon cancer is higher than that of right colon cancer. The histopathological classification is mainly adenocarcinoma, and the ratio of early stage is high.
基金Supported by Grand Innovating Program of Agriculture Applying Technique in Shandong Province (No.2008-109)Commonweal Project of State Oceanic Administration (No. 200705023)
文摘Broodstock of Verasper moseri （Jordan and Gilbert） aged 3-4 years old were selected, and reinforced cultivation was conducted to promote maturation under controlled water temperature and photoperiod conditions. Fertilized eggs were obtained by artificial fertilization, and the development of embryos, larvae and juveniles was observed continuously. The results showed that the fertilized eggs of V. moseri were spherical, with transparent yolk and homogeneous bioplasm, and had no oil globule inside. The average diameter of the eggs was 1.77＋0.02 mm. The eggs of V. moseri were buoyant in water with salinity above 35. The cleavage type was typical discoidal. Young pigment cells appeared when olfactory plates began to form. Hatching occurred at 187 h after fertilization at a water temperature of 8.5℃. The newly hatched larvae, floating on the water surface, were transparent with an average total length of 4.69±0.15 mm. During the cultivation period, when the water temperature was raised from 9 to 14.5℃, 4-day old larvae showed more melanophores on the body surface, making the larvae gray in color. The pectoral fins began to develop, which enabled the larvae to swim horizontally and in a lively manner. On days 7-8, the digestive duct formed. The yolk sac was small and black. The yolk sac was absorbed on day 11. Larvae took food actively, and body length and body height clearly increased. The rudiments of dorsal and anal fin pterygiophores were discernible and caudal fin ray elements formed on day 19. On day 24, the larval notochord flexed upwards, and the rays of unpaired fins began to differentiate. Pigment cells converged on the dorsal and anal fin rays, and the mastoid teeth on the mandible appeared. On day 29, the left eyes of juveniles began to move upwards. Depigmentation began in some juveniles and they became sandy brown in color on day 37. Most juveniles began to settle on the bottom of the tank. The left eyes of juveniles migrated completely to the right side on day 50, when the average body length attained 2.5±0.18 cm, and juveniles accomplished metamorphosis to young. The embryonic and larval characters of several flounder species are compared.
基金National Basic Research Program of China(2003CB314906)
文摘A single-mode optical fiber with a convex chromatic dispersion profile is proposed for generating a flat supercontinuum(SC).The fiber has normal dispersion and the dispersion parameter D(λ,z) is a convex function of wavelengths.It is shown from the numerical results that the chromatic dispersion,the flatness of the dispersion curve and the pump conditions have significant effect on SC generation.A flat and broad SC without strong residual pump component can be obtained when the pump wavelength is set in the vicinity of the wavelength at which the fiber has small normal group-velocity dispersion(GVD) and small dispersion slope.The fiber with a smaller normal GVD,a flatter dispersion profile and a higher nonlinear coefficient are more suitable for broad SC generation.
