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D8S1179位点扩增不平衡
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作者 吕德坚 陈玉川 刘秋玲 《法医学杂志》 CAS CSCD 2002年第1期38-39,共2页
关键词 D8S1179位点扩增不平衡 遗传标记 亲子鉴定 银染法
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乳酸脱氢酶催化反应的热力学研究 被引量:2
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作者 刘秀萍 徐晓莹 +2 位作者 范业鹏 许琪 周凌云 《哈尔滨医科大学学报》 CAS 2002年第5期375-377,共3页
目的 探讨乳酸脱氢酶 (LDH)催化反应的热力学。方法 用Sigma公司生产的LDH ,在生化标准态 (pH7.0 ,2 5℃ ,10 1.3kPa)测定出其催化反应的平衡常数 (Keq) ,吉布斯自由能 (△G0′) ,将以上数值与用热力学公式(△G0′=-nF△E0′,△G0′=-... 目的 探讨乳酸脱氢酶 (LDH)催化反应的热力学。方法 用Sigma公司生产的LDH ,在生化标准态 (pH7.0 ,2 5℃ ,10 1.3kPa)测定出其催化反应的平衡常数 (Keq) ,吉布斯自由能 (△G0′) ,将以上数值与用热力学公式(△G0′=-nF△E0′,△G0′=-RT1nKeq)计算出的Keq及△G0′相比较。结果 用酶反应测出的Keq =1.1× 10 1 0 mol L ,△G0′=- 5 7.1KJ mol;用热力学公式计算出的Keq =2 .5× 10 4 mol L ,△G0′ =- 2 5 .0 9KJ mol,差异非常显著。结论为了获得Keq及△G0′ 数值 ,应该用Keq =V1 KiQKmp V2 KiAKmB ,△G0′ =-V1 KiQKmp V2 KiAKmB 或Keq =[P][Q] [A][B],△G0′=-RT1nKeq来求取Keq。 展开更多
关键词 催化反应 乳酸脱氢酶 热力学 平衡位点
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Effect of Silicon-on-Insulator Substrate on Residual Strain in 3C-SiC Films
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作者 王晓峰 黄风义 +5 位作者 孙国胜 王雷 赵万顺 曾一平 李海鸥 段晓峰 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2005年第9期1681-1687,共7页
One group of SiC films are grown on silicon-on-insulator (SOI) substrates with a series of silicon-overlayer thickness. Raman scattering spectroscopy measurement clearly indicates that a systematic trend of residual... One group of SiC films are grown on silicon-on-insulator (SOI) substrates with a series of silicon-overlayer thickness. Raman scattering spectroscopy measurement clearly indicates that a systematic trend of residual stress reduction as the silicon over-layer thickness decreases for the SOI substrates. Strain relaxation in the SiC epilayer is explained by force balance approach and near coincidence lattice model. 展开更多
关键词 RAMAN strain relaxation force balance principle near coincidence lattice model
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Genetic diversity and population structure of a Sichuan sika deer(Cervus sichuanicus) population in Tiebu Nature Reserve based on microsatellite variation 被引量:1
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作者 Ya HE Zheng-Huan WANG Xiao-Ming WANG 《Zoological Research》 CAS CSCD 北大核心 2014年第6期528-536,共9页
Cervus sichuanicus is a species of sika deer (Cervus nippon Group). To date, research has mainly focused on quantity surveying and behavior studies, with genetic information on this species currently deficient. To p... Cervus sichuanicus is a species of sika deer (Cervus nippon Group). To date, research has mainly focused on quantity surveying and behavior studies, with genetic information on this species currently deficient. To provide scientific evidence to assist in the protection of this species, we collected Sichuan sika deer fecal samples from the Sichuan Tiebu Nature Reserve (TNR) and extracted DNA from those samples. Microsatellite loci of bovine were used for PCR amplification. After GeneScan, the genotype data were used to analyze the genetic diversity and population structure of the Sichuan sika deer in TNR. Results showed that the average expected heterozygosity of the Sichuan sika deer population in TNR was 0.562, equivalent to the average expected heterozygosity of endangered animals, such as Procapra przewalsldi. Furthermore, 8 of 9 microsatellite loci significantly deviated from the Hardy-Weinberg equilibrium and two groups existed within the Sichuan sika deer TNR population. This genetic structure may be caused by a group of Manchurian sika deer (Cervus hortulorum) released in TNR. 展开更多
关键词 Sichuan sika deer MICROSATELLITE Genetic diversity Population structure
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Genetic diversity and differentiation of masu salmon(Oncorhynchus masou masou) between and within cultured populations inferred from microsatellite DNA analysis 被引量:2
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作者 Zhiying JI Yuyong ZHANG +1 位作者 Shuqiang CHEN Lianyu SHI 《Zoological Research》 CAS CSCD 北大核心 2012年第4期I0005-I0011,共7页
Masu salmon,Oncorhynchus masou masou,is one of the most valuable fishery species that has been introduced to China,though to date no studies on the genetic diversity and genetic relationship among hatchery populations... Masu salmon,Oncorhynchus masou masou,is one of the most valuable fishery species that has been introduced to China,though to date no studies on the genetic diversity and genetic relationship among hatchery populations has been performed with molecular markers.We undertook such a study and sampled 120 individuals from three hatchery stocks and analyzed 20 microsatellite loci.All loci were polymorphic and a total of 91 alleles were detected.A relatively low level of genetic diversity was revealed with effective number of allele of 3.1094,3.3299 and 3.1894 and expected heterozygosity of 0.6600,0.6648 and 0.6638 in the three stocks,respectively.Deviations from Hardy-Weinberg equilibrium were found due to heterozygote deficit.Accordingly,evidence of genetic bottlenecks were found in the three stocks.An individual assignment test demonstrated that 85% of individuals were correctly assigned into their original stocks.Pairwise Fst revealed that significant differentiation occurred between these three stocks.The results of the study indicated that disequilibrium of genetic structure and differentiation has occurred in all three stocks.This information collectively provides a basis for measures to avoid of loss of genetic diversity and introgression in Chinese aquaculture. 展开更多
关键词 Oncorhynchus masou masou MICROSATELLITE Genetic diversity Genetic differentiation
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A neutral-point potential balance control strategy for three-level inverter based on VSVPWM
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作者 CHENG Dong-liang WANG Xiao-peng +1 位作者 ZHU Tian-liang MA Wen-gang 《Journal of Measurement Science and Instrumentation》 CAS CSCD 2018年第4期397-404,共8页
The topology of diode neutral-point-clamped(NPC)three-level inverter is prone to neutral-point potential offset.When the sum of three-phase current is zero,the virtual space vector pulse width modulation(VSVPWM)scheme... The topology of diode neutral-point-clamped(NPC)three-level inverter is prone to neutral-point potential offset.When the sum of three-phase current is zero,the virtual space vector pulse width modulation(VSVPWM)scheme does not cause the neutral-point voltage offset,but it lacks the ability to balance the deviation.For this reason,a neutral-point potential control strategy combining virtual space vector modulation and loop width control is proposed.The neutral-point potential is balanced by introducing the distribution factor for the regions with redundant vectors.For other regions,the potential is controlled by selecting a suitable switching sequence.Meanwhile,the effect on the virtual vector modulation is reduced within the loop width by setting an appropriate loop width,thereby improving the balance effect.The simulation results show that the proposed method has a strong ability to control the offset and has excellent potential balance performance under the conditions of balanced load,unbalanced load and asymmetric capacitance parameters. 展开更多
关键词 three-level inverter virtual space vector pulse width modulation(VSVPWM) neutral-point potential balance switching sequence
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