The rice Eui （ELONGATED UPPERMOST INTERNODE） gene encodes a cytochrome P450 monooxygenase that deactivates bioactive gibberellins （GAs）. In this study, we investigated controlled expression of the Eui gene and its...The rice Eui （ELONGATED UPPERMOST INTERNODE） gene encodes a cytochrome P450 monooxygenase that deactivates bioactive gibberellins （GAs）. In this study, we investigated controlled expression of the Eui gene and its role in plant development. We found that Eui was differentially induced by exogenous GAs and that the Eui promoter had the highest activity in the vascular bundles. The eui mutant was defective in starch granule development in root caps and Eui overexpression enhanced starch granule generation and gravity responses, revealing a role for GA in root starch granule development and gravity responses. Experiments using embryoless half-seeds revealed that RAmylA and GAmyb were highly upregulated in eui aleurone ceils in the absence of exogenous GA. In addition, the GA biosynthesis genes GA3oxl and GA20ox2 were downregulated and GA2oxl was upregulated in eui seedlings. These results indicate that EUI is involved in GA homeostasis, not only in the internodes at the heading stage, but also in the seedling stage, roots and seeds. Disturbing GA homeostasis affected the expression of the GA signaling genes GID1 （GIBBERELLIN INSENSITIVE DWARF 1）, GID2 and SLR1. Transgenic RNA interference of the Eui gene effectively increased plant height and improved heading performance. By contrast, the ectopic expression of Eui under the promoters of the rice GA biosynthesis genes GA3ox2 and GA2Oox2 significantly reduced plant height. These results demonstrate that a slight increase in Eui expression could dramatically change rice morphology, indicating the practical application of the Eui gene in rice molecular breeding for a high yield potential.展开更多
Cantilever casting concrete arch bridge using form traveller has a broad application prospect.However,it is difficult to obtain reasonable initial cable force in construction stage.In this study,stress balance and inf...Cantilever casting concrete arch bridge using form traveller has a broad application prospect.However,it is difficult to obtain reasonable initial cable force in construction stage.In this study,stress balance and influence matrix methods were developed to determine the initial cable force of cantilever casting concrete arch bridge.The stress balance equation and influence matrix of arch rib critical section were established,and the buckle cable force range was determined by the allowable stress of arch rib critical section.Then a group of buckle cable forces were selected and substituted into the stress balance equation,and the reasonable initial buckle cable force was determined through iteration.Based on the principle of force balance,the initial anchor cable force was determined.In an engineering application example,it is shown that the stress balance and influence matrix methods for the determination of initial cable force are feasible and reliable.The initial cable forces of arch rib segments only need to be adjusted once in the corresponding construction process,which improves the working efficiency and reduces the construction risk.It is found that the methods have great advantages for determining initial cable force in cantilever casting construction process of concrete arch bridge.展开更多
The bridge piles located in high-steep slopes not only endure the loads from superstructure, but also the residual sliding force as well as the resistance from the slope. By introducing the Winkler foundation theory, ...The bridge piles located in high-steep slopes not only endure the loads from superstructure, but also the residual sliding force as well as the resistance from the slope. By introducing the Winkler foundation theory, the mechanical model of piles-soils-slopes system was established, and the equilibrium differential equations of pile were derived. Moreover, an analytic solution for identifying the model parameters was provided by means of power series method. A project with field measurement was compared with the proposed method. It is indicated that the lateral loads have great influences on the pile, the steep slope effect is indispensable, and reasonable diameter of the pile could enhance the bending ability. The internal force and displacements of pile are largely based upon the horizontal loads applied on pile, especially in upper part.展开更多
基金Abbreviations： double-stranded RNA （dsRNA） ELONGATED UPPER- MOST INTERNODE （Eui）+5 种基金 gibberellin （GA） GIBBERELLIN INSENSI- TIVE DWARF （GID） overexpression （OX） RNA interference （RNAi） slender rice （SLR） wild type （WT） We are grateful to Dr Shinjiro Yamaguchi （RIKEN, Ja- pan） for critical reading of the manuscript, and to Professor Yinong Yang （Penn. State University, USA） for the rice RNAi vector. This work was supported by grants from the National Natural Science Foundation of China （30670186 and 30421001）, and the Ministry of Science and Technology of China （2006AA10A102） to ZH.
文摘The rice Eui （ELONGATED UPPERMOST INTERNODE） gene encodes a cytochrome P450 monooxygenase that deactivates bioactive gibberellins （GAs）. In this study, we investigated controlled expression of the Eui gene and its role in plant development. We found that Eui was differentially induced by exogenous GAs and that the Eui promoter had the highest activity in the vascular bundles. The eui mutant was defective in starch granule development in root caps and Eui overexpression enhanced starch granule generation and gravity responses, revealing a role for GA in root starch granule development and gravity responses. Experiments using embryoless half-seeds revealed that RAmylA and GAmyb were highly upregulated in eui aleurone ceils in the absence of exogenous GA. In addition, the GA biosynthesis genes GA3oxl and GA20ox2 were downregulated and GA2oxl was upregulated in eui seedlings. These results indicate that EUI is involved in GA homeostasis, not only in the internodes at the heading stage, but also in the seedling stage, roots and seeds. Disturbing GA homeostasis affected the expression of the GA signaling genes GID1 （GIBBERELLIN INSENSITIVE DWARF 1）, GID2 and SLR1. Transgenic RNA interference of the Eui gene effectively increased plant height and improved heading performance. By contrast, the ectopic expression of Eui under the promoters of the rice GA biosynthesis genes GA3ox2 and GA2Oox2 significantly reduced plant height. These results demonstrate that a slight increase in Eui expression could dramatically change rice morphology, indicating the practical application of the Eui gene in rice molecular breeding for a high yield potential.
基金Projects(51478049,51778068)supported by the National Natural Science Foundation of ChinaProject(14JJ2075,2019JJ40301)supported by the Hunan Natural Science Foundation of China+1 种基金Project(17A010)supported by the Scientific Research Fund of Hunan Provincial Education Department of ChinaProject(2017GK4034)supported by the Major Technological Achievements Transformation Program of Hunan Strategic Emerging Industries of China
文摘Cantilever casting concrete arch bridge using form traveller has a broad application prospect.However,it is difficult to obtain reasonable initial cable force in construction stage.In this study,stress balance and influence matrix methods were developed to determine the initial cable force of cantilever casting concrete arch bridge.The stress balance equation and influence matrix of arch rib critical section were established,and the buckle cable force range was determined by the allowable stress of arch rib critical section.Then a group of buckle cable forces were selected and substituted into the stress balance equation,and the reasonable initial buckle cable force was determined through iteration.Based on the principle of force balance,the initial anchor cable force was determined.In an engineering application example,it is shown that the stress balance and influence matrix methods for the determination of initial cable force are feasible and reliable.The initial cable forces of arch rib segments only need to be adjusted once in the corresponding construction process,which improves the working efficiency and reduces the construction risk.It is found that the methods have great advantages for determining initial cable force in cantilever casting construction process of concrete arch bridge.
基金Project(51408066)supported by the National Natural Science Foundation of China
文摘The bridge piles located in high-steep slopes not only endure the loads from superstructure, but also the residual sliding force as well as the resistance from the slope. By introducing the Winkler foundation theory, the mechanical model of piles-soils-slopes system was established, and the equilibrium differential equations of pile were derived. Moreover, an analytic solution for identifying the model parameters was provided by means of power series method. A project with field measurement was compared with the proposed method. It is indicated that the lateral loads have great influences on the pile, the steep slope effect is indispensable, and reasonable diameter of the pile could enhance the bending ability. The internal force and displacements of pile are largely based upon the horizontal loads applied on pile, especially in upper part.
