Objective The aim was to explore callus induction and plant regeneration of perennial ryegrass, as well as provide the foundation for transgenic research on perennial ryegrass.[ Methed] Mature seeds of perennial ryegr...Objective The aim was to explore callus induction and plant regeneration of perennial ryegrass, as well as provide the foundation for transgenic research on perennial ryegrass.[ Methed] Mature seeds of perennial ryegrass were used as explants to study the effects of different hormone compositions on callus induction, proliferation and plant differentiation. Result The result showed that the induction rate achieved its highest on 2,4-D of 8 mg/L combining with 6-BA of 0.025 mg/L, which was up to 56.42%. Callus were differentiated after two to three generations, the highest differentiation rate 34.14% was achieved in the medium contained MS medium with 6-BA of 2 mg/L, and the differentiation rate was obviously affected by the callus condition after proliferation. The root inducing medium, containing 0.5 mg/L NAA and MS medium with half of macroelement, gained 98% root inducing rate. Conclusien A high frequency genetic regeneration system was established.展开更多
Lepidium meyeniiWalp. （Maca）, a perennial herbaceous plant with narrow distdbution in the Andean region, was cultivated by local residents as early as 1600 B.C. It has high nutritional values and multiple medicinal ...Lepidium meyeniiWalp. （Maca）, a perennial herbaceous plant with narrow distdbution in the Andean region, was cultivated by local residents as early as 1600 B.C. It has high nutritional values and multiple medicinal effects. In recent years the plant source of Maca has been greatly scarce since Maca products are increasingly becoming popular in the world. This makes the study urgent on the identification and quality evaluation of Maca materials. In present work, four Mace samples of different origins or handlings and three other natural plants, including radish, odental ginseng and American ginseng, were investigated. Their essential oils （steam distillates） of the hypogeal parts were weighed and analyzed by GC/MS. Through comparison of the characteristics of their chromatograms, it was found that the essential oil of Maca had unique GC/MS behaviors, and the characteristic peaks produced by the major essential oil components （Phenylacetonitrile, Benzaldehyde, 3-Methoxyphenylacetonitrile, etc） were distinct. Furthermore, the chromatograms of several Maca samples showed a similar qualitative pattern except for some differences in the quantitative composition, and the percentage of total essential oils in the samples varied from 0.88 % to 1.59 %. These behaviors and technologies can be applied to the identification of Maca materials in the markets and be a supplement to assess the quality of Maca matedals.展开更多
The age-structure of natural population of Zoysia japonica in Xiuyan County of Liaoning Province was studied by generational method. The results showed that the highest tiller age class was three, but 1st age class ti...The age-structure of natural population of Zoysia japonica in Xiuyan County of Liaoning Province was studied by generational method. The results showed that the highest tiller age class was three, but 1st age class tillers held dominant posi-tion with proportions over 95% in each month during the growing seasons. The 2nd age class and 3rd age class tillers were minority in the population. So Z. japonica population was an expanding population. The zero age class buds on the rhizomes were dominant in buds age structures. The proportion of buds to tillers on quantity in each month was about 30% to 40% and reached the highest at the end of September. The increasing of buds proportion before dormancy guaranteed the quantity of tillers in the next spring. The biomass of 1st age class tillers changed with time. The biomass kept increasing from April to July and reached the highest at the end of July and then decreased.展开更多
[Objective] The countermeasure on the number of fructification of Spartina alterniflora in the period of sexual reproduction and the component of seed yielding construction was explored.[Method] The Spartina altemiflo...[Objective] The countermeasure on the number of fructification of Spartina alterniflora in the period of sexual reproduction and the component of seed yielding construction was explored.[Method] The Spartina altemiflora in Mangroves conservation zone located at Hepu of Guangxi being taken as experimental material, its morphological and quantitative characteristics, as well as the weight of 100 full seeds at maturity stage in three different growth conditions（ clay, loam and sand） were studied. [ Results] The results showed that Spartina alterniflora had the best growth pattern in the loam. The morphological factors of fructification of S. altemiflora grown in sand were larger then in others. In the three growth conditions the order of quantitative characteristics of fructification of S. alterniflora was clay 〉 sand 〉 loam and the seeds in spikelet at top position were more maturity than those at the button position. [ Conclusion] In good condition, the Spartina altemiflora growth was vigor but the ratio of seed-setting was low.展开更多
[Objective] This study aimed to investigate the effect of potyamine priming on physiological and biochemical variations of Lolium perenne embryos and seed germination. [Method] With annual Lolium perenne （Diamond T a...[Objective] This study aimed to investigate the effect of potyamine priming on physiological and biochemical variations of Lolium perenne embryos and seed germination. [Method] With annual Lolium perenne （Diamond T and Grazing-8000） as experimental materials, after priming with 0.5 mmol/L putrescine （Put）, spermidine （Spd） and spermine （Spm） for 24 h and chilling imbibition at 5 ℃ for 12, 24, 36 and 48 h, the effect of Put, Spd and Spm priming on chilling tolerance and germination ability of annual Lolium perenne seeds during imbibition was investigated. [Result] Put, Spd and Spm priming improved the activities of peroxidase （POD）, catalase （CAT）, superoxide dismutase （SOD） and ascorbate peroxidase （APX） and content of soluble protein content under low temperature stress, significantly in-creased the germination rate, and shortened the average germination duration. After chilling imbibition for 48 h, compared with the control, the average germination rate of annual Lolium perenne seeds was improved by 15.5% and 12.0% after Put, Spd and Spm priming, and the average germination duration was shortened by 1.21 and 1.14 d. During seed imbibition, the chilling tolerance of Grazing-8000 was stronger than that of Diamond T. Overall, Put, Spd and Spm treatment could increase the chilling tolerance of annual Lolium perenne seeds during imbibition, and improve the germination ability of seeds under low temperature stress. [Conclusion] Results of this study provided theoretical basis for the application of seed priming technology in the production of annual ryegrass.展开更多
Ventral globus pallidus-injured rats by kainic acid(10 mg) were used to derter-mine the monoamine levels in four different brain regions.In this model,a great decrease of NE con-centration was observed in both hippoca...Ventral globus pallidus-injured rats by kainic acid(10 mg) were used to derter-mine the monoamine levels in four different brain regions.In this model,a great decrease of NE con-centration was observed in both hippocampus and frontal cortex compared with nonnal contro.This result hints that a damage of noradrenergic neurons in this model has occurred. DA concentrations in the four brain zones after kainic acid injection were all reduced, but only in the frontal cortex and striatum the pronounced reductions were discovered while DA turnover rates in frontal cortex, stria-tum and meddullapons were significantly reduced. These results revealed a DA metabolic disorder occurring in these regions.However,5-HT concentrations as well as DBH activity, expressed by ratio of NE/DA,showed no marked difference in this model. In our study it is found that the changes of monoamine levels in this model basically reflect those discovered in AD patients.展开更多
Agave americana Var. Marginata Hort. （Agavaceae） is a perennial herb. This paper summarized its classification, distribution, morphological characteristics, habit, flowering and fruiting, cultivation and reproductio...Agave americana Var. Marginata Hort. （Agavaceae） is a perennial herb. This paper summarized its classification, distribution, morphological characteristics, habit, flowering and fruiting, cultivation and reproduction, as well as multiple uses in daily life, which provided references for the exploitation and utilization of Agave americana Vat. Marginata Hort. （Agavaceae）.展开更多
The compositions of soilless culture media for indoor lawns were studied through the determination of quality indexes （grass height, clipping weight, leaf width, density, lawn color and uniformity） of Festuca rubra ...The compositions of soilless culture media for indoor lawns were studied through the determination of quality indexes （grass height, clipping weight, leaf width, density, lawn color and uniformity） of Festuca rubra L. and Perennial Lolzum perenne L lawns. The results showed that composition 4 （black turf 1.2 L＋vermiculite 0.3 L＋ceramsite 0 L） is not suitable for the cultivation of indoor lawns. Composition 1 （black turf 0.3 L＋vermiculite 0.75 L＋ceramsite 0.45 L） and composition 2 （black turf 0.6 L＋vermiculite 0.6 L＋ceramsite 0.3 L） are suitable for indoor cultivation of F. rubra; and Perennial L. perenne turned yellow in all the tested media, and therefore, the media tested in this study are not suitable for indoor cultivation of Perennial L. perenne.展开更多
Based on data from 22 sample plots and applying the Canonical Correspondence Analysis （CCA）, this paper discusses the vegetation-environment relationships between the northern slope of Karlik Mountain and Naomaohu B...Based on data from 22 sample plots and applying the Canonical Correspondence Analysis （CCA）, this paper discusses the vegetation-environment relationships between the northern slope of Karlik Mountain and Naomaohu Ba- sin, which is situated in the easternmost end of the Tianshan Mountains, Xinjiang Uygur Autonomous Region, China. For the zonal vegetation, community diversity of mountain vegetation is higher than that of the desert vegetation due to environmental factors. The CCA ordination diagram revealed that the composition and distribution of vegetation types are mainly determined by altitude, soil pH and soil salt content. With increasing elevation, the soil pH and total salt content decrease but the contents of soil organic matter, soil water, total nitrogen and total phosphorus increase gradu- ally. In the CCA ordination diagrams, the sample plots and main species can be divided into five types according to their adaptations to the environmental factors. Type Ⅰ is composed of desert vegetation distributed on the low moun- tains, hills, plains and deserts below an elevation of 1900 m; type Ⅱ is distributed in the mountain and desert ecotone with an elevation of 1900-2300 m, and includes steppe desert, desert steppe and wetland meadow; type Ⅲ is very sim- ply composed of only salinized meadow; type Ⅳ is distributed above an elevation of 2300 m, containing mountain steppe, meadow steppe, subalpine meadow and alpine meadow; type Ⅴ only contains salinized meadow. The results show that with increasing elevation, species combination changes from the xerophytic shrubs, semi-shrubs and herbs distributed in the low altitude zone with arid climate to the cold-tolerant perennial herbs growing in the high altitudinal zone with cold climate.展开更多
The vegetation of alpine tundra in the Changbai Mountains has experienced great changes in recent decades. Narrowleaf small reed(Deyeuxia angustifolia), a perennial herb from the birch forest zone had crossed the tree...The vegetation of alpine tundra in the Changbai Mountains has experienced great changes in recent decades. Narrowleaf small reed(Deyeuxia angustifolia), a perennial herb from the birch forest zone had crossed the tree line and invaded into the alpine tundra zone. To reveal the driven mechanism of D. angustifolia invasion, there is an urgent need to figure out the effective seed distribution pattern, which could tell us where the potential risk regions are and help us to interpret the invasion process. In this study, we focus on the locations of the seeds in the soil layer and mean to characterize the effective seed distribution pattern of D. angustifolia. The relationship between the environmental variables and the effective seed distribution pattern was also assessed by redundancy analysis. Results showed that seeds of D. angustifolia spread in the alpine tundra with a considerable number(mean value of 322 per m2). They were mainly distributed in the low elevation areas with no significant differences in different slope positions. Effective seed number(ESN) occurrences of D. angustifolia were different in various plant communities. Plant communities with lower canopy cover tended to have more seeds of D. angustifolia. Our research indicated reliable quantitative information on the extent to which habitats are susceptible to invasion.展开更多
基金Supported by National Natural Science Foundation of China(30471274)~~
文摘Objective The aim was to explore callus induction and plant regeneration of perennial ryegrass, as well as provide the foundation for transgenic research on perennial ryegrass.[ Methed] Mature seeds of perennial ryegrass were used as explants to study the effects of different hormone compositions on callus induction, proliferation and plant differentiation. Result The result showed that the induction rate achieved its highest on 2,4-D of 8 mg/L combining with 6-BA of 0.025 mg/L, which was up to 56.42%. Callus were differentiated after two to three generations, the highest differentiation rate 34.14% was achieved in the medium contained MS medium with 6-BA of 2 mg/L, and the differentiation rate was obviously affected by the callus condition after proliferation. The root inducing medium, containing 0.5 mg/L NAA and MS medium with half of macroelement, gained 98% root inducing rate. Conclusien A high frequency genetic regeneration system was established.
文摘Lepidium meyeniiWalp. （Maca）, a perennial herbaceous plant with narrow distdbution in the Andean region, was cultivated by local residents as early as 1600 B.C. It has high nutritional values and multiple medicinal effects. In recent years the plant source of Maca has been greatly scarce since Maca products are increasingly becoming popular in the world. This makes the study urgent on the identification and quality evaluation of Maca materials. In present work, four Mace samples of different origins or handlings and three other natural plants, including radish, odental ginseng and American ginseng, were investigated. Their essential oils （steam distillates） of the hypogeal parts were weighed and analyzed by GC/MS. Through comparison of the characteristics of their chromatograms, it was found that the essential oil of Maca had unique GC/MS behaviors, and the characteristic peaks produced by the major essential oil components （Phenylacetonitrile, Benzaldehyde, 3-Methoxyphenylacetonitrile, etc） were distinct. Furthermore, the chromatograms of several Maca samples showed a similar qualitative pattern except for some differences in the quantitative composition, and the percentage of total essential oils in the samples varied from 0.88 % to 1.59 %. These behaviors and technologies can be applied to the identification of Maca materials in the markets and be a supplement to assess the quality of Maca matedals.
基金This study was supported by the NKBRSF (G1999043407-1) Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX2-406+3 种基金 SCXZD0101) NKTRDP (2001BA510B-07 2002BA516A20) and Education Committee Projects of Liaong Province (990121400
文摘The age-structure of natural population of Zoysia japonica in Xiuyan County of Liaoning Province was studied by generational method. The results showed that the highest tiller age class was three, but 1st age class tillers held dominant posi-tion with proportions over 95% in each month during the growing seasons. The 2nd age class and 3rd age class tillers were minority in the population. So Z. japonica population was an expanding population. The zero age class buds on the rhizomes were dominant in buds age structures. The proportion of buds to tillers on quantity in each month was about 30% to 40% and reached the highest at the end of September. The increasing of buds proportion before dormancy guaranteed the quantity of tillers in the next spring. The biomass of 1st age class tillers changed with time. The biomass kept increasing from April to July and reached the highest at the end of July and then decreased.
基金Supported by the National Natural Science Foundation of China(30660036 )the Natural Science Foundation of Guangxi Province(0728096) Project of Graduate Student Education Innovation ofGuangxi (2008106020907M266)~~
文摘[Objective] The countermeasure on the number of fructification of Spartina alterniflora in the period of sexual reproduction and the component of seed yielding construction was explored.[Method] The Spartina altemiflora in Mangroves conservation zone located at Hepu of Guangxi being taken as experimental material, its morphological and quantitative characteristics, as well as the weight of 100 full seeds at maturity stage in three different growth conditions（ clay, loam and sand） were studied. [ Results] The results showed that Spartina alterniflora had the best growth pattern in the loam. The morphological factors of fructification of S. altemiflora grown in sand were larger then in others. In the three growth conditions the order of quantitative characteristics of fructification of S. alterniflora was clay 〉 sand 〉 loam and the seeds in spikelet at top position were more maturity than those at the button position. [ Conclusion] In good condition, the Spartina altemiflora growth was vigor but the ratio of seed-setting was low.
基金Supported by Agricultural Research Project of Guizhou Province([2010]No.3045)~~
文摘[Objective] This study aimed to investigate the effect of potyamine priming on physiological and biochemical variations of Lolium perenne embryos and seed germination. [Method] With annual Lolium perenne （Diamond T and Grazing-8000） as experimental materials, after priming with 0.5 mmol/L putrescine （Put）, spermidine （Spd） and spermine （Spm） for 24 h and chilling imbibition at 5 ℃ for 12, 24, 36 and 48 h, the effect of Put, Spd and Spm priming on chilling tolerance and germination ability of annual Lolium perenne seeds during imbibition was investigated. [Result] Put, Spd and Spm priming improved the activities of peroxidase （POD）, catalase （CAT）, superoxide dismutase （SOD） and ascorbate peroxidase （APX） and content of soluble protein content under low temperature stress, significantly in-creased the germination rate, and shortened the average germination duration. After chilling imbibition for 48 h, compared with the control, the average germination rate of annual Lolium perenne seeds was improved by 15.5% and 12.0% after Put, Spd and Spm priming, and the average germination duration was shortened by 1.21 and 1.14 d. During seed imbibition, the chilling tolerance of Grazing-8000 was stronger than that of Diamond T. Overall, Put, Spd and Spm treatment could increase the chilling tolerance of annual Lolium perenne seeds during imbibition, and improve the germination ability of seeds under low temperature stress. [Conclusion] Results of this study provided theoretical basis for the application of seed priming technology in the production of annual ryegrass.
文摘Ventral globus pallidus-injured rats by kainic acid(10 mg) were used to derter-mine the monoamine levels in four different brain regions.In this model,a great decrease of NE con-centration was observed in both hippocampus and frontal cortex compared with nonnal contro.This result hints that a damage of noradrenergic neurons in this model has occurred. DA concentrations in the four brain zones after kainic acid injection were all reduced, but only in the frontal cortex and striatum the pronounced reductions were discovered while DA turnover rates in frontal cortex, stria-tum and meddullapons were significantly reduced. These results revealed a DA metabolic disorder occurring in these regions.However,5-HT concentrations as well as DBH activity, expressed by ratio of NE/DA,showed no marked difference in this model. In our study it is found that the changes of monoamine levels in this model basically reflect those discovered in AD patients.
文摘Agave americana Var. Marginata Hort. （Agavaceae） is a perennial herb. This paper summarized its classification, distribution, morphological characteristics, habit, flowering and fruiting, cultivation and reproduction, as well as multiple uses in daily life, which provided references for the exploitation and utilization of Agave americana Vat. Marginata Hort. （Agavaceae）.
文摘The compositions of soilless culture media for indoor lawns were studied through the determination of quality indexes （grass height, clipping weight, leaf width, density, lawn color and uniformity） of Festuca rubra L. and Perennial Lolzum perenne L lawns. The results showed that composition 4 （black turf 1.2 L＋vermiculite 0.3 L＋ceramsite 0 L） is not suitable for the cultivation of indoor lawns. Composition 1 （black turf 0.3 L＋vermiculite 0.75 L＋ceramsite 0.45 L） and composition 2 （black turf 0.6 L＋vermiculite 0.6 L＋ceramsite 0.3 L） are suitable for indoor cultivation of F. rubra; and Perennial L. perenne turned yellow in all the tested media, and therefore, the media tested in this study are not suitable for indoor cultivation of Perennial L. perenne.
基金Under the auspices of National Natural Science Foundation of China(No.41171157)
文摘Based on data from 22 sample plots and applying the Canonical Correspondence Analysis （CCA）, this paper discusses the vegetation-environment relationships between the northern slope of Karlik Mountain and Naomaohu Ba- sin, which is situated in the easternmost end of the Tianshan Mountains, Xinjiang Uygur Autonomous Region, China. For the zonal vegetation, community diversity of mountain vegetation is higher than that of the desert vegetation due to environmental factors. The CCA ordination diagram revealed that the composition and distribution of vegetation types are mainly determined by altitude, soil pH and soil salt content. With increasing elevation, the soil pH and total salt content decrease but the contents of soil organic matter, soil water, total nitrogen and total phosphorus increase gradu- ally. In the CCA ordination diagrams, the sample plots and main species can be divided into five types according to their adaptations to the environmental factors. Type Ⅰ is composed of desert vegetation distributed on the low moun- tains, hills, plains and deserts below an elevation of 1900 m; type Ⅱ is distributed in the mountain and desert ecotone with an elevation of 1900-2300 m, and includes steppe desert, desert steppe and wetland meadow; type Ⅲ is very sim- ply composed of only salinized meadow; type Ⅳ is distributed above an elevation of 2300 m, containing mountain steppe, meadow steppe, subalpine meadow and alpine meadow; type Ⅴ only contains salinized meadow. The results show that with increasing elevation, species combination changes from the xerophytic shrubs, semi-shrubs and herbs distributed in the low altitude zone with arid climate to the cold-tolerant perennial herbs growing in the high altitudinal zone with cold climate.
基金Special Fund of National Seismological Bureau,China(No.201208005)Doctorial Innovation Fund of Northeast Normal University(No.10SSXT133,2412013XS001)+1 种基金National Natural Science Foundation of China(No.41171038,41171072,41101523)Doctoral Fund of Ministry of Education of China(No.20120043110014)
文摘The vegetation of alpine tundra in the Changbai Mountains has experienced great changes in recent decades. Narrowleaf small reed(Deyeuxia angustifolia), a perennial herb from the birch forest zone had crossed the tree line and invaded into the alpine tundra zone. To reveal the driven mechanism of D. angustifolia invasion, there is an urgent need to figure out the effective seed distribution pattern, which could tell us where the potential risk regions are and help us to interpret the invasion process. In this study, we focus on the locations of the seeds in the soil layer and mean to characterize the effective seed distribution pattern of D. angustifolia. The relationship between the environmental variables and the effective seed distribution pattern was also assessed by redundancy analysis. Results showed that seeds of D. angustifolia spread in the alpine tundra with a considerable number(mean value of 322 per m2). They were mainly distributed in the low elevation areas with no significant differences in different slope positions. Effective seed number(ESN) occurrences of D. angustifolia were different in various plant communities. Plant communities with lower canopy cover tended to have more seeds of D. angustifolia. Our research indicated reliable quantitative information on the extent to which habitats are susceptible to invasion.
