年轻红细胞(Young Red Blood Cell简称YRBC)是一种具有网织红细胞数量和酶活性相对增高，且平均细胞年龄较小的‘种新的血液成分．它对于长期依赖输血的重型地中海贫血患者和各类长期输血的贫血患者的输注，能延长输血间隔期和减少输血...年轻红细胞(Young Red Blood Cell简称YRBC)是一种具有网织红细胞数量和酶活性相对增高，且平均细胞年龄较小的‘种新的血液成分．它对于长期依赖输血的重型地中海贫血患者和各类长期输血的贫血患者的输注，能延长输血间隔期和减少输血量，进而达到减低体在体内的累积速率，而国内至今尚未见有同类报道。本文采用手工法分离制备YRBC制品并应用于长期依赖输血的5例血液痫患者，替代常规输血，结果较为满意，现报告如下。展开更多
目的减少污染,使年轻红细胞(Young red blood cell,YRBC)制品更具有安全性。方法使用改良的密闭三连袋制备年轻红细胞制品。结果改良前后制备的YRBC均符合质控标准。结论改良后的方法避免了开放性操作步骤,减少了污染,使YRBC制品的安全...目的减少污染,使年轻红细胞(Young red blood cell,YRBC)制品更具有安全性。方法使用改良的密闭三连袋制备年轻红细胞制品。结果改良前后制备的YRBC均符合质控标准。结论改良后的方法避免了开放性操作步骤,减少了污染,使YRBC制品的安全性更具有保障,同时简化工作程序,节约时间,提高工作效率。展开更多
OBJECTIVE To determine the quantity and quality of liver NK cells from young and adult mice and compare their characteristics. METHODS C57BL/6 mice were used at 2 weeks （young） and 8 weeks （adult） of age. The perc...OBJECTIVE To determine the quantity and quality of liver NK cells from young and adult mice and compare their characteristics. METHODS C57BL/6 mice were used at 2 weeks （young） and 8 weeks （adult） of age. The percentage and absolute number of NK cells in the liver and spleen were analyzed. The cytotoxicity of NK cells in the liver and spleen against various targets were detected by a 4 h ^51Cr-release method. FACScan was used to analyze the expression of CD69, Mac-l1 Ly49C/I and CD94 on the NK cells. Perforin mRNA levels were analyzed by the reverse transcription polymerase chain reaction （RT-PCR）. RESUTLS The percentages of NK cells in the liver of young and adult mice were similar （11.9%＋1.7% vs. 9.9%＋1.6%, P〉0.05）, but the absolute number per liver weight was higher in the young animals （11.6＋2.5×10^5/g vs. 3.4＋0.8×10^5/g, P〈0.05）. The level of NK cytotoxicity was extremely high in the liver of young compared to adult mice （71.0%＋5.5% vs. 23.8%＋4.4%, P〈0.05）, but this difference was not observed in the spleen. Phenotypes of the liver NK cells from young and adult mice were completely different from each other. The liver NK cells from young mice were CD69^high Mac-1^low Ly49C/ I^low, whereas NK cells from older mice displayed inverse antigen levels （CD- 69^low Mac-1^high Ly49C/I^high）. The expression levels of other NK cell-related markers were similar in both groups.The perforin mRNA level in the liver lymphocytes from young mice was consistently greater compared to adult mice. CONCLUSION From 2 to 8 weeks C57BU6 mice liver NK cells undergo age-associated changes. At 2 weeks of age the liver NK cells showed a high level of NK cytotoxicity and a unique phenotype which was not apparent at 8 weeks of age.展开更多
文摘年轻红细胞(Young Red Blood Cell简称YRBC)是一种具有网织红细胞数量和酶活性相对增高，且平均细胞年龄较小的‘种新的血液成分．它对于长期依赖输血的重型地中海贫血患者和各类长期输血的贫血患者的输注，能延长输血间隔期和减少输血量，进而达到减低体在体内的累积速率，而国内至今尚未见有同类报道。本文采用手工法分离制备YRBC制品并应用于长期依赖输血的5例血液痫患者，替代常规输血，结果较为满意，现报告如下。
文摘目的减少污染,使年轻红细胞(Young red blood cell,YRBC)制品更具有安全性。方法使用改良的密闭三连袋制备年轻红细胞制品。结果改良前后制备的YRBC均符合质控标准。结论改良后的方法避免了开放性操作步骤,减少了污染,使YRBC制品的安全性更具有保障,同时简化工作程序,节约时间,提高工作效率。
文摘OBJECTIVE To determine the quantity and quality of liver NK cells from young and adult mice and compare their characteristics. METHODS C57BL/6 mice were used at 2 weeks （young） and 8 weeks （adult） of age. The percentage and absolute number of NK cells in the liver and spleen were analyzed. The cytotoxicity of NK cells in the liver and spleen against various targets were detected by a 4 h ^51Cr-release method. FACScan was used to analyze the expression of CD69, Mac-l1 Ly49C/I and CD94 on the NK cells. Perforin mRNA levels were analyzed by the reverse transcription polymerase chain reaction （RT-PCR）. RESUTLS The percentages of NK cells in the liver of young and adult mice were similar （11.9%＋1.7% vs. 9.9%＋1.6%, P〉0.05）, but the absolute number per liver weight was higher in the young animals （11.6＋2.5×10^5/g vs. 3.4＋0.8×10^5/g, P〈0.05）. The level of NK cytotoxicity was extremely high in the liver of young compared to adult mice （71.0%＋5.5% vs. 23.8%＋4.4%, P〈0.05）, but this difference was not observed in the spleen. Phenotypes of the liver NK cells from young and adult mice were completely different from each other. The liver NK cells from young mice were CD69^high Mac-1^low Ly49C/ I^low, whereas NK cells from older mice displayed inverse antigen levels （CD- 69^low Mac-1^high Ly49C/I^high）. The expression levels of other NK cell-related markers were similar in both groups.The perforin mRNA level in the liver lymphocytes from young mice was consistently greater compared to adult mice. CONCLUSION From 2 to 8 weeks C57BU6 mice liver NK cells undergo age-associated changes. At 2 weeks of age the liver NK cells showed a high level of NK cytotoxicity and a unique phenotype which was not apparent at 8 weeks of age.
