The intensive management practices in greenhouse production may alter the soil physicochemical properties and contribute to the accumulation of heavy metals(HMs). To determine the HM concentrations in vegetable soil i...The intensive management practices in greenhouse production may alter the soil physicochemical properties and contribute to the accumulation of heavy metals(HMs). To determine the HM concentrations in vegetable soil in relation to soil physicochemical properties and cultivation age, we conducted a soil survey for typical greenhouse soils in Shouguang, China. The results indicated that Cd is a major HM pollutant in the tested soils, as the only HM element exceeding the allowed limit for vegetable soil. The surveyed data was analyzed with regression analysis, correlation analysis and canonical correspondence analysis(CCA). A positive correlation is observed between HM pollution level and cultivation age. CCA results suggest that the HM pollution level and distribution in soil are significantly affected by soil physicochemical properties, which was a function of years of cultivation as revealed by regression analysis. In summary, cultivation age is an important factor to affect soil physicochemical properties(organic matter and inorganic nutrients) as well as HM contamination.展开更多
Objective To investigate the mutations of mitochondrial genome in a pedigree with suspected maternally inherited diabetes and deafness and to explore the correlations between the mutations and clinical features. Meth...Objective To investigate the mutations of mitochondrial genome in a pedigree with suspected maternally inherited diabetes and deafness and to explore the correlations between the mutations and clinical features. Methods Genomic DNA was isolated from blood leucocytes of each member of the pedigree. The mitochondrial genome was amplified with 24-pair primers that could cover the entire mitochondrial DNA. Direct sequencing of PCR products was used to identify any mitochondrial DNA mutations. Results Family members on the maternal side all harbored the tRNA^Lcu（UUR） A3243G mutation. The paternal side family members did not have the mutation. The age-of-onset of diabetes of the 4 maternal side family members was 15, 41, 44, and 65 years old, and their corresponding heteroplasmy level of the mutation was 34.5%, 14.9%, 14.6%, and 5.9%, respectively. The age-of-onset of diabetes and heteroplasmy level of A3243G mutation were negatively correlated with a correlation coefficient of -0.980（P=0.02）. Meanwhile, patient with high heteroplasmy level of A3243G mutation had relatively low severity of disease. Moreover, 6 reported polymorphisms and 2 new variants were found. Conclusions The main cause of diabetes in this pedigree is the tRNA^Lcu（UUR） A3243G mutation. However, other gene variants may contribute to its pathogenicity. The heteroplasmy level of the tRNA^Lcu（UUR） A3243G mutation is positively associated with earlier age-of-onset and increasing severity of diabetes.展开更多
On the north piedmont of Tianshan Mountains, China, the Kuytun River and Manas River transverse the Dushanzi and Manas folds, resulted in 7-level and 6-level pedestal terraces, respectively, which are the tectonomorph...On the north piedmont of Tianshan Mountains, China, the Kuytun River and Manas River transverse the Dushanzi and Manas folds, resulted in 7-level and 6-level pedestal terraces, respectively, which are the tectonomorphic marks of folding and uplift of the Dushanzi and Manas anticlines since the late Quaternary. We have collected samples from deposits of all terraces for OSL （optically stimulated luminescence） geological dating using the SMAR （single-multiple-aliquot-regeneration） method on fine grains. We have also performed dating using the ~4C method on the samples from the deposit of terrace T1 along the Kuy- tun River. The results show that all these deposits are of the later phase of the late Pleistocene. Comparison of terrace dating and climate change since 200 ka suggests that the terraces TI, T2, T3, T4 and T5 along the Manas River formed in 6, 8.5, 10, 14 and 32 ka, respectively. The incision time of Kuitun River＇s T1, T2, T3, T4, T5, T6 and T7 terraces were 1.7, 14, 20, 25, 32 50 and 100 ka years ago, respectively. The terrace T4 along the Manas River and T2 along the Kuytun River were formed dur- ing the late part of the late Pleistocene, i.e., 14 ka. Since 14 ka, the incision of the Manas River has generated three levels of pedestal terraces, while that of the Kuytun River has only produced one level of such terraces. The latest folding and uplift of the Dushanzi anticline took place in 1.7 ka, while that of the Manas anticline occurred in 5 ka. Since 14ka or the later time of the late Pleistocene, the Dushanzi and Manas anticlines have risen by 40 and 95 m, respectively, implying uplift rates 2.7 and 6.8 mm/a for each.展开更多
基金Under the auspices of National Key Research and Development Program of China(No.2016YFD0800304)Natural Science Foundation of Shandong(No.ZR2017MD023,ZR2018BD003)
文摘The intensive management practices in greenhouse production may alter the soil physicochemical properties and contribute to the accumulation of heavy metals(HMs). To determine the HM concentrations in vegetable soil in relation to soil physicochemical properties and cultivation age, we conducted a soil survey for typical greenhouse soils in Shouguang, China. The results indicated that Cd is a major HM pollutant in the tested soils, as the only HM element exceeding the allowed limit for vegetable soil. The surveyed data was analyzed with regression analysis, correlation analysis and canonical correspondence analysis(CCA). A positive correlation is observed between HM pollution level and cultivation age. CCA results suggest that the HM pollution level and distribution in soil are significantly affected by soil physicochemical properties, which was a function of years of cultivation as revealed by regression analysis. In summary, cultivation age is an important factor to affect soil physicochemical properties(organic matter and inorganic nutrients) as well as HM contamination.
文摘Objective To investigate the mutations of mitochondrial genome in a pedigree with suspected maternally inherited diabetes and deafness and to explore the correlations between the mutations and clinical features. Methods Genomic DNA was isolated from blood leucocytes of each member of the pedigree. The mitochondrial genome was amplified with 24-pair primers that could cover the entire mitochondrial DNA. Direct sequencing of PCR products was used to identify any mitochondrial DNA mutations. Results Family members on the maternal side all harbored the tRNA^Lcu（UUR） A3243G mutation. The paternal side family members did not have the mutation. The age-of-onset of diabetes of the 4 maternal side family members was 15, 41, 44, and 65 years old, and their corresponding heteroplasmy level of the mutation was 34.5%, 14.9%, 14.6%, and 5.9%, respectively. The age-of-onset of diabetes and heteroplasmy level of A3243G mutation were negatively correlated with a correlation coefficient of -0.980（P=0.02）. Meanwhile, patient with high heteroplasmy level of A3243G mutation had relatively low severity of disease. Moreover, 6 reported polymorphisms and 2 new variants were found. Conclusions The main cause of diabetes in this pedigree is the tRNA^Lcu（UUR） A3243G mutation. However, other gene variants may contribute to its pathogenicity. The heteroplasmy level of the tRNA^Lcu（UUR） A3243G mutation is positively associated with earlier age-of-onset and increasing severity of diabetes.
基金supported by National Natural Science Foundation of China (Grant No. 40572126)Special Project of Seismological Community (Grant No. 200808013)
文摘On the north piedmont of Tianshan Mountains, China, the Kuytun River and Manas River transverse the Dushanzi and Manas folds, resulted in 7-level and 6-level pedestal terraces, respectively, which are the tectonomorphic marks of folding and uplift of the Dushanzi and Manas anticlines since the late Quaternary. We have collected samples from deposits of all terraces for OSL （optically stimulated luminescence） geological dating using the SMAR （single-multiple-aliquot-regeneration） method on fine grains. We have also performed dating using the ~4C method on the samples from the deposit of terrace T1 along the Kuy- tun River. The results show that all these deposits are of the later phase of the late Pleistocene. Comparison of terrace dating and climate change since 200 ka suggests that the terraces TI, T2, T3, T4 and T5 along the Manas River formed in 6, 8.5, 10, 14 and 32 ka, respectively. The incision time of Kuitun River＇s T1, T2, T3, T4, T5, T6 and T7 terraces were 1.7, 14, 20, 25, 32 50 and 100 ka years ago, respectively. The terrace T4 along the Manas River and T2 along the Kuytun River were formed dur- ing the late part of the late Pleistocene, i.e., 14 ka. Since 14 ka, the incision of the Manas River has generated three levels of pedestal terraces, while that of the Kuytun River has only produced one level of such terraces. The latest folding and uplift of the Dushanzi anticline took place in 1.7 ka, while that of the Manas anticline occurred in 5 ka. Since 14ka or the later time of the late Pleistocene, the Dushanzi and Manas anticlines have risen by 40 and 95 m, respectively, implying uplift rates 2.7 and 6.8 mm/a for each.
