This study describes the complete diallel hybridization between newly introduced bay scallop stock(W) from Canada and local commercial stock(D) grown under laboratory conditions, in China. Larval survival and growth d...This study describes the complete diallel hybridization between newly introduced bay scallop stock(W) from Canada and local commercial stock(D) grown under laboratory conditions, in China. Larval survival and growth during all life stages(larvae, spat, and adult) were compared among hybrid(DW, WD) and purebred(DD, WW) populations. Signifi cant heterosis was detected for survival during the larval stage(>1% of the mid-parent values). The mean heterosis( H m) varied in growth throughout the life span. More than 50% of the H m values were positive and negative in the DW and WD groups, respectively. The infl uence of maternal effects and mating types(intrapopulation vs. interpopulation crosses) on growth for all life stages was not consistent. Larval survival did not differ signifi cantly( P >0.05) with maternal effect or mating type. In the harvest stage, shell length(SL), shell height(SH), shell width(SW), and total weight(TW) were larger in the hybrid compared with the inbred groups. Positive H m values were observed in SL(1.5%), SW(5.8%), and TW(12.3%), and were more signifi cant in the DW groups(6.1%, 4.5%, 6.8%, and 27.2%). These results suggest that hybridization between two geographic populations is a good tool for improving bay scallop growth. However, unstable heterosis between the two populations requires further study.展开更多
Low larval survival,poor settlement,and abnormal metamorphosis are major problems in seed production of donkey-ear abalone Haliotis asinina.We examined the effects of chemical cues including epinephrine,nor-epinephrin...Low larval survival,poor settlement,and abnormal metamorphosis are major problems in seed production of donkey-ear abalone Haliotis asinina.We examined the effects of chemical cues including epinephrine,nor-epinephrine,and serotonin on larval survival,settlement,and metamorphosis in order to determine the possibility of using these chemicals to induce the problems.The results show that epinephrine could enhance metamorphosis rate at 10-6 mol/L only but higher concentrations(10-3-10-4 mol/L);and nor-epinephrine could inhibit the performance significantly,and serotonin could increase significantly the performance at a wide-range concentration(10-3-10-6 mol/L).Treatment with serotonin at 10-5 mol/L for 72 hours resulted in the highest settlement rate(42.2%) and survival rate(49.3%),while at 10-4 mol/L for 72 hours resulted in the highest metamorphosis rate(38.8%).Therefore,serotonin may be used as a fast metamorphosis inducer in abalone culture.展开更多
Trumpet shell, Charonia sauliae, is an endangered and valuable species, but its artificial propagation protocol has not been successfully established. To estimate the possibility of cryopreservation for larvae of C. s...Trumpet shell, Charonia sauliae, is an endangered and valuable species, but its artificial propagation protocol has not been successfully established. To estimate the possibility of cryopreservation for larvae of C. sauliae, which is a potential preparation for its artificial reproduction and further research, in this study a protocol for the cryopreservation of veliger larvae of trumpet shell was optimized. Through a two-step cryopreservation procedure, four kinds of cryoprotectants (ethylene glycol, 1, 2-propanediol, dimethyl sulfoxide and glycerol) were employed at three concentrations (1.0, 1.5 and 2.0 molL-1) respectively and survival rates of larvae were determined after a storage of 1h. The larvae frozen with these four cryoprotectants after 1 h storage were cultured, and then survival rates were determined at 24, 72 and 120 h after thawing. Dimethyl sulfoxide at a concentration of 1.5 molL-1 showed the best protective effect in all experiments (p<0.05). And survival rates of larvae frozen with dimethyl sulfoxide were determined after 1, 7 and 15 d of storage. The survival rates of larvae frozen with 1.5 molL-1 dimethyl sulfoxide after 1 h, 1 d, 7 d and 15 d of storage were 80.77% ±7.51%, 80.34% ±11.28%, 83.10% ±9.14% and 77.23% ±6.22% respectively. No significant differences in survival rates of larvae frozen with dimethyl sulfoxide were observed after various storage periods (p>0.05).展开更多
基金Supported by the National High Technology Research and Development Program of China(863 Program)(Nos.2012AA10A410,2010AA10A401)the National Natural Science Foundation of China(No.30800842)+2 种基金the Mollusc Research and Development Center(No.CARS-48)the Taishan Scholar Program of Shandong Provincethe Climbing Program of Taishan Scholars of Shandong Province
文摘This study describes the complete diallel hybridization between newly introduced bay scallop stock(W) from Canada and local commercial stock(D) grown under laboratory conditions, in China. Larval survival and growth during all life stages(larvae, spat, and adult) were compared among hybrid(DW, WD) and purebred(DD, WW) populations. Signifi cant heterosis was detected for survival during the larval stage(>1% of the mid-parent values). The mean heterosis( H m) varied in growth throughout the life span. More than 50% of the H m values were positive and negative in the DW and WD groups, respectively. The infl uence of maternal effects and mating types(intrapopulation vs. interpopulation crosses) on growth for all life stages was not consistent. Larval survival did not differ signifi cantly( P >0.05) with maternal effect or mating type. In the harvest stage, shell length(SL), shell height(SH), shell width(SW), and total weight(TW) were larger in the hybrid compared with the inbred groups. Positive H m values were observed in SL(1.5%), SW(5.8%), and TW(12.3%), and were more signifi cant in the DW groups(6.1%, 4.5%, 6.8%, and 27.2%). These results suggest that hybridization between two geographic populations is a good tool for improving bay scallop growth. However, unstable heterosis between the two populations requires further study.
基金Support by the Key Scientific and Technological Project of Hainan Province (No. 080137)the Key Project of University (No. hd09×m10)
文摘Low larval survival,poor settlement,and abnormal metamorphosis are major problems in seed production of donkey-ear abalone Haliotis asinina.We examined the effects of chemical cues including epinephrine,nor-epinephrine,and serotonin on larval survival,settlement,and metamorphosis in order to determine the possibility of using these chemicals to induce the problems.The results show that epinephrine could enhance metamorphosis rate at 10-6 mol/L only but higher concentrations(10-3-10-4 mol/L);and nor-epinephrine could inhibit the performance significantly,and serotonin could increase significantly the performance at a wide-range concentration(10-3-10-6 mol/L).Treatment with serotonin at 10-5 mol/L for 72 hours resulted in the highest settlement rate(42.2%) and survival rate(49.3%),while at 10-4 mol/L for 72 hours resulted in the highest metamorphosis rate(38.8%).Therefore,serotonin may be used as a fast metamorphosis inducer in abalone culture.
文摘Trumpet shell, Charonia sauliae, is an endangered and valuable species, but its artificial propagation protocol has not been successfully established. To estimate the possibility of cryopreservation for larvae of C. sauliae, which is a potential preparation for its artificial reproduction and further research, in this study a protocol for the cryopreservation of veliger larvae of trumpet shell was optimized. Through a two-step cryopreservation procedure, four kinds of cryoprotectants (ethylene glycol, 1, 2-propanediol, dimethyl sulfoxide and glycerol) were employed at three concentrations (1.0, 1.5 and 2.0 molL-1) respectively and survival rates of larvae were determined after a storage of 1h. The larvae frozen with these four cryoprotectants after 1 h storage were cultured, and then survival rates were determined at 24, 72 and 120 h after thawing. Dimethyl sulfoxide at a concentration of 1.5 molL-1 showed the best protective effect in all experiments (p<0.05). And survival rates of larvae frozen with dimethyl sulfoxide were determined after 1, 7 and 15 d of storage. The survival rates of larvae frozen with 1.5 molL-1 dimethyl sulfoxide after 1 h, 1 d, 7 d and 15 d of storage were 80.77% ±7.51%, 80.34% ±11.28%, 83.10% ±9.14% and 77.23% ±6.22% respectively. No significant differences in survival rates of larvae frozen with dimethyl sulfoxide were observed after various storage periods (p>0.05).
