应用石蜡切片技术和显微测量法，对从孵化后到变态完成（55～60d，day post hatching，dph）的条斑星鲽（Verasper moseri Jordan et Gilbert）仔稚幼鱼早期发育中消化道的结构变化进行了观察，描述了个体发育过程中的组织学结构特征。...应用石蜡切片技术和显微测量法，对从孵化后到变态完成（55～60d，day post hatching，dph）的条斑星鲽（Verasper moseri Jordan et Gilbert）仔稚幼鱼早期发育中消化道的结构变化进行了观察，描述了个体发育过程中的组织学结构特征。结果表明，在13～15℃，7dph的仔鱼开口时，消化道已经分化为口咽腔、食道、肠和直肠，消化器官肝脏和胰腺出现。从7dph到14～17dph（卵黄囊消失）为混合营养期，形态上肠道进一步弯曲、盘旋；功能上中肠空泡样结构和后肠嗜伊红颗粒的出现，预示着肠上皮细胞在胃功能成熟前有吸收蛋白的作用。形态学上胃以及幽门盲囊的分化出现在26～29dph，与稚鱼刚开始附底的时期相吻合。而功能性胃的分化则出现在35dph左右，与稚鱼变态期相一致。条斑星鲽作为中国新近养殖品种，关于其早期发育的报道还是空白，作者对其消化系统进行系统的研究，旨在为该鱼种的商业化养殖提供相关的科学数据，同时也为海水鱼类早期营养和发育生物学的深入研究提供理论依据。展开更多
AIM： To investigate the hypothesis that duodenal bulb （DB） inhibition on pyloric antrum （PA） contraction is reflex. METHODS： Balloon （condom）-tipped tube was introduced into 1^st duodenum （DD） and a manometr...AIM： To investigate the hypothesis that duodenal bulb （DB） inhibition on pyloric antrum （PA） contraction is reflex. METHODS： Balloon （condom）-tipped tube was introduced into 1^st duodenum （DD） and a manometric tube into each of PA and DD. Duodenal and antral pressure response to duodenal and then PA balloon distension with saline was recorded. These tests were repeated after separate anesthetization of DD and PA. RESULTS： Two and 4 mL of 1^st DD balloon distension produced no pressure changes in DD or PA （10.7±1.2 vs 9.8±1.2, 11.2±1.2 vs 11.3±1.2 on H20 respectively, P〉0.05）. Six mL distension effected 1^st DD pressure rise （30.6±3.4 cm H20, P 〈0.01） and PA pressure decrease （6.2±1.4 cm H20, P〈0.05）; no response in 2^nd, 3^rd and 4^th DD. There was no difference between 6, 8, and 10 mL distensions. Ten mL PA distension produced no PA or 1^st DD pressure changes （P〉 0.05）. Twenty mL distension increased PA pressure （92.4 4±10.7 cm H20, P〈0.01） and decreased 1^st DD pressure （1.6±0.3 cm H20, P〈0.01）; 30, 40, and 50 mL distension produced the same effect as the 20 mL distension （P 〉 0.05）. PA or DD distension after separate anesthetization produced no significant pressure changes in PA or DD. CONCLUSION： Large volume DD distension produced DD pressure rise denoting DD contraction and PA pressure decline denoting PA relaxation. PA relaxation upon DD contraction is postulated to be mediated through a reflex which we call duodeno-antral reflex. Meanwhile, PA distension effected DD relaxation which we suggest to be reflex and termed antro-duodenal reflex. It is suggested that these 2 reflexes, could act as investigative tools in diagnosis of gastroduodenal motility disorders.展开更多
文摘应用石蜡切片技术和显微测量法，对从孵化后到变态完成（55～60d，day post hatching，dph）的条斑星鲽（Verasper moseri Jordan et Gilbert）仔稚幼鱼早期发育中消化道的结构变化进行了观察，描述了个体发育过程中的组织学结构特征。结果表明，在13～15℃，7dph的仔鱼开口时，消化道已经分化为口咽腔、食道、肠和直肠，消化器官肝脏和胰腺出现。从7dph到14～17dph（卵黄囊消失）为混合营养期，形态上肠道进一步弯曲、盘旋；功能上中肠空泡样结构和后肠嗜伊红颗粒的出现，预示着肠上皮细胞在胃功能成熟前有吸收蛋白的作用。形态学上胃以及幽门盲囊的分化出现在26～29dph，与稚鱼刚开始附底的时期相吻合。而功能性胃的分化则出现在35dph左右，与稚鱼变态期相一致。条斑星鲽作为中国新近养殖品种，关于其早期发育的报道还是空白，作者对其消化系统进行系统的研究，旨在为该鱼种的商业化养殖提供相关的科学数据，同时也为海水鱼类早期营养和发育生物学的深入研究提供理论依据。
文摘AIM： To investigate the hypothesis that duodenal bulb （DB） inhibition on pyloric antrum （PA） contraction is reflex. METHODS： Balloon （condom）-tipped tube was introduced into 1^st duodenum （DD） and a manometric tube into each of PA and DD. Duodenal and antral pressure response to duodenal and then PA balloon distension with saline was recorded. These tests were repeated after separate anesthetization of DD and PA. RESULTS： Two and 4 mL of 1^st DD balloon distension produced no pressure changes in DD or PA （10.7±1.2 vs 9.8±1.2, 11.2±1.2 vs 11.3±1.2 on H20 respectively, P〉0.05）. Six mL distension effected 1^st DD pressure rise （30.6±3.4 cm H20, P 〈0.01） and PA pressure decrease （6.2±1.4 cm H20, P〈0.05）; no response in 2^nd, 3^rd and 4^th DD. There was no difference between 6, 8, and 10 mL distensions. Ten mL PA distension produced no PA or 1^st DD pressure changes （P〉 0.05）. Twenty mL distension increased PA pressure （92.4 4±10.7 cm H20, P〈0.01） and decreased 1^st DD pressure （1.6±0.3 cm H20, P〈0.01）; 30, 40, and 50 mL distension produced the same effect as the 20 mL distension （P 〉 0.05）. PA or DD distension after separate anesthetization produced no significant pressure changes in PA or DD. CONCLUSION： Large volume DD distension produced DD pressure rise denoting DD contraction and PA pressure decline denoting PA relaxation. PA relaxation upon DD contraction is postulated to be mediated through a reflex which we call duodeno-antral reflex. Meanwhile, PA distension effected DD relaxation which we suggest to be reflex and termed antro-duodenal reflex. It is suggested that these 2 reflexes, could act as investigative tools in diagnosis of gastroduodenal motility disorders.
