High-performance liquid chromatography with UV detection was used for the quantification of the flavonoid quercetin, the active compound found in ＂Guangdong Wang-bu-liu-xing＂. The method was developed and demonstrat...High-performance liquid chromatography with UV detection was used for the quantification of the flavonoid quercetin, the active compound found in ＂Guangdong Wang-bu-liu-xing＂. The method was developed and demonstrated to provide superior performance over other commonly documented methods. This HPLC assay achieved high specificity through the use of a reversed-phase C18 column eluted with a mobile phase of methanol-0.4% （v/v） phosphoric acid （50：50, v/v） over the course of 30 min. UV detection at 360 nm was used. This analytical method provided excellent precision and a mean recovery of 99%, demonstrated by repeated analysis of 11 sample groups. Because of its high performance and simplicity, this HPLC assay can be readily utilized as a practical method for the quality control of active compounds extracted from Guangdong Wang-bu-liu-xing.展开更多
文摘High-performance liquid chromatography with UV detection was used for the quantification of the flavonoid quercetin, the active compound found in ＂Guangdong Wang-bu-liu-xing＂. The method was developed and demonstrated to provide superior performance over other commonly documented methods. This HPLC assay achieved high specificity through the use of a reversed-phase C18 column eluted with a mobile phase of methanol-0.4% （v/v） phosphoric acid （50：50, v/v） over the course of 30 min. UV detection at 360 nm was used. This analytical method provided excellent precision and a mean recovery of 99%, demonstrated by repeated analysis of 11 sample groups. Because of its high performance and simplicity, this HPLC assay can be readily utilized as a practical method for the quality control of active compounds extracted from Guangdong Wang-bu-liu-xing.
