[ Objective] To study the characteristics of florescence and pollen in Lagerstroemia speciosa. [ Method ] The process of flower opening and pollen tube germination of Lagerstroemia speciosa was observed and the pollen...[ Objective] To study the characteristics of florescence and pollen in Lagerstroemia speciosa. [ Method ] The process of flower opening and pollen tube germination of Lagerstroemia speciosa was observed and the pollen viability was determined through in vitro germination. [ Result] Sepals of L. speciosa started to diverge at 4：30 am, at 7： 00 am petals flatten up, anther diverged, and the stigma secreted a large number of mucus, it was the best time for artificial pollination. Boric acid and sucrose had a great effect on in vitro pollen germination of L. speciosa, the combination which made highest rate of pollen germination, was sucrose 150 g/L ＋ boric acid 20 mg/L ＋ CaCI2 10 mg/L. Through the fluorescence microscope, it was known that four hours after flowering, a lot of pollen grains germinated on the stigma, six hours after flowering, lots of pollen tubes entered the style and reached to 1/4 length of the style, 12 hours after flowering, pollen tubes concentrated into a beam forward, and reached to 1/2 length of the style, 24 hours after flowering, lots of pollen tubes entered the ovary in a beam and then fertilized and produced seeds. [ Conclusion] The results provide some basis for utilizing L. speciosa to breed.展开更多
基金Supported by the National Key Technology R&D Program in EleventhFive-Year Plan of China(2006BAD01A18)the Program fromMinistry of Environmental Protection of China(Species09-2-3-1)~~
文摘[ Objective] To study the characteristics of florescence and pollen in Lagerstroemia speciosa. [ Method ] The process of flower opening and pollen tube germination of Lagerstroemia speciosa was observed and the pollen viability was determined through in vitro germination. [ Result] Sepals of L. speciosa started to diverge at 4：30 am, at 7： 00 am petals flatten up, anther diverged, and the stigma secreted a large number of mucus, it was the best time for artificial pollination. Boric acid and sucrose had a great effect on in vitro pollen germination of L. speciosa, the combination which made highest rate of pollen germination, was sucrose 150 g/L ＋ boric acid 20 mg/L ＋ CaCI2 10 mg/L. Through the fluorescence microscope, it was known that four hours after flowering, a lot of pollen grains germinated on the stigma, six hours after flowering, lots of pollen tubes entered the style and reached to 1/4 length of the style, 12 hours after flowering, pollen tubes concentrated into a beam forward, and reached to 1/2 length of the style, 24 hours after flowering, lots of pollen tubes entered the ovary in a beam and then fertilized and produced seeds. [ Conclusion] The results provide some basis for utilizing L. speciosa to breed.
